Growth factor induction of nitric oxide synthase in rat pheochromocytoma cells

A. Macduff Sheehy, Yume T. Phung, R. Kirk Riemer, Stephen M. Black

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Previous work has suggested that nerve growth factor treatment of PC12 cells induces neuronal nitric oxide synthase, and possibly also endothelial nitric oxide synthase (NOS) and inducible NOS. To further analyze this process we exposed rat pheochromocytoma (PC12) cells to increasing concentrations of basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), nerve growth factor (NGF), and vascular endothelial cell growth factor (VEGF). Changes in NOS expression were then analyzed by Western blotting, using antisera generated against the three isoforms of NOS. Our results demonstrate that neuronal NOS was induced by growth factors that promote both differentiation (bFGF, NGF) and proliferation (EGF). nNOS levels were unaffected by VEGF treatment. In contrast, the levels of endothelial and inducible NOS were undetectable in these same cells, suggesting that different clonal lines of PC12 cells have different isoform complements.

Original languageEnglish (US)
Pages (from-to)71-77
Number of pages7
JournalMolecular Brain Research
Volume52
Issue number1
DOIs
StatePublished - Dec 1 1997

Fingerprint

PC12 Cells
Pheochromocytoma
Nerve Growth Factor
Nitric Oxide Synthase
Intercellular Signaling Peptides and Proteins
Nitric Oxide Synthase Type I
Nitric Oxide Synthase Type III
Nitric Oxide Synthase Type II
Fibroblast Growth Factor 2
Epidermal Growth Factor
Vascular Endothelial Growth Factor A
Protein Isoforms
Immune Sera
Western Blotting

Keywords

  • Enzyme induction
  • Growth factor
  • Nitric oxide synthase
  • PC12 cell
  • Polyclonal antiserum

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Growth factor induction of nitric oxide synthase in rat pheochromocytoma cells. / Sheehy, A. Macduff; Phung, Yume T.; Riemer, R. Kirk; Black, Stephen M.

In: Molecular Brain Research, Vol. 52, No. 1, 01.12.1997, p. 71-77.

Research output: Contribution to journalArticle

Sheehy, A. Macduff ; Phung, Yume T. ; Riemer, R. Kirk ; Black, Stephen M. / Growth factor induction of nitric oxide synthase in rat pheochromocytoma cells. In: Molecular Brain Research. 1997 ; Vol. 52, No. 1. pp. 71-77.
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