Growth factor-like phospholipids generated after corneal injury

Károly Liliom, Zhiwei Guan, Jih Lie Tseng, Dominic M. Desiderio, Gábor Tigyi, Mitchell A. Watsky

Research output: Contribution to journalArticle

96 Scopus citations

Abstract

The present study provides evidence that growth factor-like glycerophosphate mediators of the lysophosphatidic acid (LPA) family are present in the aqueous humor and the lacrimal gland fluid of the rabbit eye. By use of a combination of HPLC, two-dimensional TLC, mass spectrometry, and the Xenopus oocyte bioassay, the LPA-like phospholipids LPA, cyclic PA, alkenyl-glycerophosphate (GP), lysophosphatidylserine, and phosphatidic acid were detected as physiological constituents of the fluids bathing the cornea. Corneal injury resulted in an increased production of some of these mediators. Alkenyl-GP, a novel member of the LPA family, has been identified in postinjury aqueous humor, establishing that it is generated endogenously. LPA and its homologues were found to be mitogenic in freshly dissociated keratocytes from uninjured corneas. There appears to be a link between the occurrence of LPA responsiveness in keratocytes activated by injury and the increase in LPA-like activity in aqueous humor. These data suggest that LPA and its homologues are involved in maintaining the integrity of the normal cornea and in promoting cellular regeneration of the injured cornea.

Original languageEnglish (US)
Pages (from-to)C1065-C1074
JournalAmerican Journal of Physiology - Cell Physiology
Volume274
Issue number4 43-4
StatePublished - Apr 1 1998
Externally publishedYes

    Fingerprint

Keywords

  • Aqueous humor
  • Chloride current
  • Keratocyte
  • Lacrimal gland
  • Lipid mediator
  • Lysophosphatidic acid
  • Wound healing

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Liliom, K., Guan, Z., Tseng, J. L., Desiderio, D. M., Tigyi, G., & Watsky, M. A. (1998). Growth factor-like phospholipids generated after corneal injury. American Journal of Physiology - Cell Physiology, 274(4 43-4), C1065-C1074.