Growth inhibitory and apoptosis-inducing effects of xanthohumol, a prenylated chalone present in hops, in human prostate cancer cells

D. Deeb, X. Gao, H. Jiang, Ali Syed Arbab, S. A. Dulchavsky, Subhash C. Gautam

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Promotion of apoptosis in cancer cells could potentially lead to the regression and improved prognosis of hormone-refractory prostate cancer. Xanthohumol (XN), a prenylated chalcone-derived from hops, has shown strong antitumorigenic activity towards diverse types of cancer cells. In the present study, the growth-inhibitory and apoptosis-inducing activity of XN was tested in hormone-sensitive and hormone-refractory human prostate cancer cells lines. Cell growth/viability assay (MTS) demonstrated that prostate cancer cells are highly sensitive to XN at a concentration range of 20-40 μM. The primary mode of tumor cell destruction was apoptosis as demonstrated by the binding of annexin V-FITC, cleavage of PARP-1, activation of procaspases -3, -8, and -9, mitochondrial depolarization and release of cytochrome c from mitochondria. Induction of apoptosis by XN was associated with the inhibition of prosurvival Akt, NF-κB and mTOR signaling proteins and NF-κB-regulated anti-apoptotic Bcl-2 and survivin. These studies provide a rationale for clinical evaluation of XN for the treatment of hormone-refractory metastatic prostate cancer.

Original languageEnglish (US)
Pages (from-to)3333-3339
Number of pages7
JournalAnticancer Research
Volume30
Issue number9
StatePublished - Jan 1 2010
Externally publishedYes

Fingerprint

Chalones
Humulus
Prostatic Neoplasms
Apoptosis
Hormones
Growth
TOR Serine-Threonine Kinases
Chalcone
Neoplasms
Caspase 8
Fluorescein-5-isothiocyanate
Annexin A5
Cytochromes c
Caspase 3
Cell Survival
Mitochondria
xanthohumol
Cell Line

Keywords

  • Apoptosis
  • Prostate cancer
  • Prosurvival signaling proteins
  • Xanthohumol

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Growth inhibitory and apoptosis-inducing effects of xanthohumol, a prenylated chalone present in hops, in human prostate cancer cells. / Deeb, D.; Gao, X.; Jiang, H.; Arbab, Ali Syed; Dulchavsky, S. A.; Gautam, Subhash C.

In: Anticancer Research, Vol. 30, No. 9, 01.01.2010, p. 3333-3339.

Research output: Contribution to journalArticle

Deeb, D. ; Gao, X. ; Jiang, H. ; Arbab, Ali Syed ; Dulchavsky, S. A. ; Gautam, Subhash C. / Growth inhibitory and apoptosis-inducing effects of xanthohumol, a prenylated chalone present in hops, in human prostate cancer cells. In: Anticancer Research. 2010 ; Vol. 30, No. 9. pp. 3333-3339.
@article{38564905ea6d40aaa434991414d7e0c3,
title = "Growth inhibitory and apoptosis-inducing effects of xanthohumol, a prenylated chalone present in hops, in human prostate cancer cells",
abstract = "Promotion of apoptosis in cancer cells could potentially lead to the regression and improved prognosis of hormone-refractory prostate cancer. Xanthohumol (XN), a prenylated chalcone-derived from hops, has shown strong antitumorigenic activity towards diverse types of cancer cells. In the present study, the growth-inhibitory and apoptosis-inducing activity of XN was tested in hormone-sensitive and hormone-refractory human prostate cancer cells lines. Cell growth/viability assay (MTS) demonstrated that prostate cancer cells are highly sensitive to XN at a concentration range of 20-40 μM. The primary mode of tumor cell destruction was apoptosis as demonstrated by the binding of annexin V-FITC, cleavage of PARP-1, activation of procaspases -3, -8, and -9, mitochondrial depolarization and release of cytochrome c from mitochondria. Induction of apoptosis by XN was associated with the inhibition of prosurvival Akt, NF-κB and mTOR signaling proteins and NF-κB-regulated anti-apoptotic Bcl-2 and survivin. These studies provide a rationale for clinical evaluation of XN for the treatment of hormone-refractory metastatic prostate cancer.",
keywords = "Apoptosis, Prostate cancer, Prosurvival signaling proteins, Xanthohumol",
author = "D. Deeb and X. Gao and H. Jiang and Arbab, {Ali Syed} and Dulchavsky, {S. A.} and Gautam, {Subhash C.}",
year = "2010",
month = "1",
day = "1",
language = "English (US)",
volume = "30",
pages = "3333--3339",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "9",

}

TY - JOUR

T1 - Growth inhibitory and apoptosis-inducing effects of xanthohumol, a prenylated chalone present in hops, in human prostate cancer cells

AU - Deeb, D.

AU - Gao, X.

AU - Jiang, H.

AU - Arbab, Ali Syed

AU - Dulchavsky, S. A.

AU - Gautam, Subhash C.

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Promotion of apoptosis in cancer cells could potentially lead to the regression and improved prognosis of hormone-refractory prostate cancer. Xanthohumol (XN), a prenylated chalcone-derived from hops, has shown strong antitumorigenic activity towards diverse types of cancer cells. In the present study, the growth-inhibitory and apoptosis-inducing activity of XN was tested in hormone-sensitive and hormone-refractory human prostate cancer cells lines. Cell growth/viability assay (MTS) demonstrated that prostate cancer cells are highly sensitive to XN at a concentration range of 20-40 μM. The primary mode of tumor cell destruction was apoptosis as demonstrated by the binding of annexin V-FITC, cleavage of PARP-1, activation of procaspases -3, -8, and -9, mitochondrial depolarization and release of cytochrome c from mitochondria. Induction of apoptosis by XN was associated with the inhibition of prosurvival Akt, NF-κB and mTOR signaling proteins and NF-κB-regulated anti-apoptotic Bcl-2 and survivin. These studies provide a rationale for clinical evaluation of XN for the treatment of hormone-refractory metastatic prostate cancer.

AB - Promotion of apoptosis in cancer cells could potentially lead to the regression and improved prognosis of hormone-refractory prostate cancer. Xanthohumol (XN), a prenylated chalcone-derived from hops, has shown strong antitumorigenic activity towards diverse types of cancer cells. In the present study, the growth-inhibitory and apoptosis-inducing activity of XN was tested in hormone-sensitive and hormone-refractory human prostate cancer cells lines. Cell growth/viability assay (MTS) demonstrated that prostate cancer cells are highly sensitive to XN at a concentration range of 20-40 μM. The primary mode of tumor cell destruction was apoptosis as demonstrated by the binding of annexin V-FITC, cleavage of PARP-1, activation of procaspases -3, -8, and -9, mitochondrial depolarization and release of cytochrome c from mitochondria. Induction of apoptosis by XN was associated with the inhibition of prosurvival Akt, NF-κB and mTOR signaling proteins and NF-κB-regulated anti-apoptotic Bcl-2 and survivin. These studies provide a rationale for clinical evaluation of XN for the treatment of hormone-refractory metastatic prostate cancer.

KW - Apoptosis

KW - Prostate cancer

KW - Prosurvival signaling proteins

KW - Xanthohumol

UR - http://www.scopus.com/inward/record.url?scp=77958563399&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77958563399&partnerID=8YFLogxK

M3 - Article

VL - 30

SP - 3333

EP - 3339

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 9

ER -