Growth inhibitory and apoptosis-inducing effects of xanthohumol, a prenylated chalone present in hops, in human prostate cancer cells

D. Deeb, X. Gao, H. Jiang, Ali Syed Arbab, S. A. Dulchavsky, Subhash C. Gautam

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Promotion of apoptosis in cancer cells could potentially lead to the regression and improved prognosis of hormone-refractory prostate cancer. Xanthohumol (XN), a prenylated chalcone-derived from hops, has shown strong antitumorigenic activity towards diverse types of cancer cells. In the present study, the growth-inhibitory and apoptosis-inducing activity of XN was tested in hormone-sensitive and hormone-refractory human prostate cancer cells lines. Cell growth/viability assay (MTS) demonstrated that prostate cancer cells are highly sensitive to XN at a concentration range of 20-40 μM. The primary mode of tumor cell destruction was apoptosis as demonstrated by the binding of annexin V-FITC, cleavage of PARP-1, activation of procaspases -3, -8, and -9, mitochondrial depolarization and release of cytochrome c from mitochondria. Induction of apoptosis by XN was associated with the inhibition of prosurvival Akt, NF-κB and mTOR signaling proteins and NF-κB-regulated anti-apoptotic Bcl-2 and survivin. These studies provide a rationale for clinical evaluation of XN for the treatment of hormone-refractory metastatic prostate cancer.

Original languageEnglish (US)
Pages (from-to)3333-3339
Number of pages7
JournalAnticancer Research
Volume30
Issue number9
Publication statusPublished - Jan 1 2010
Externally publishedYes

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Keywords

  • Apoptosis
  • Prostate cancer
  • Prosurvival signaling proteins
  • Xanthohumol

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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