Growth/differentiation factor-5 significantly enhances periodontal wound healing/regeneration compared with platelet-derived growth factor-BB in dogs

Hyuk Rak Kwon; Ulf M E Wikesjö; Jung Chul Park; Young Taek Kim; Patrizia Bastone; Susanne D. Pippig; Chong Kwan Kim

doi:10.1111/j.1600-051X.2010.01576.x

Growth/differentiation factor-5 significantly enhances periodontal wound healing/regeneration compared with platelet-derived growth factor-BB in dogs

Hyuk Rak Kwon, Ulf M E Wikesjö, Jung Chul Park, Young Taek Kim, Patrizia Bastone, Susanne D. Pippig, Chong Kwan Kim

Periodontics

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Objective: Recombinant human growth/differentiation factor-5 (rhGDF-5) in a particulate β-tricalcium phosphate (β-TCP) carrier is being evaluated to support periodontal regeneration. The objective of this study was to evaluate periodontal wound healing/regeneration following an established clinical (benchmark) protocol including surgical implantation of rhGDF-5/β-TCP in comparison with that following implantation of recombinant human platelet-derived growth factor-BB (rhPDGF) combined with a particulate β-TCP biomaterial using an established canine defect model. Materials and Methods: Bilateral, 4 × 5 mm (width × depth), one-wall, critical-size, intrabony periodontal defects were surgically created at the mandibular second and fourth pre-molar teeth in five adult Beagle dogs. Defect sites were randomized to receive rhGDF-5/β-TCP or the rhPDGF construct followed by wound closure for primary intention healing. The animals were sacrificed following an 8-week healing interval for histological and histometric examination. Results: Clinical healing was generally uneventful. Sites receiving rhGDF-5/β-TCP exhibited a significantly enhanced cementum formation compared with sites receiving the rhPDGF construct, averaging (±SD) 4.49±0.48 versus 2.72±0.91 mm (p<0.001). Similarly, bone regeneration height and area were significantly enhanced at sites receiving rhGDF-5/β-TCP versus that of the rhPDGF construct averaging, 3.08±0.74 versus 1.29±0.78 mm (p<0.001) and 6.03±1.28 versus 2.98±2.61 mm² (p<0.01), respectively. Cementum regeneration included cellular/acellular mixed (extrinsic/intrinsic) fibre cementum at sites receiving rhGDF-5/β-TCP; sites receiving the rhPDGF/β-TCP showed a pre-dominantly acellular cementum. Newly formed cementum generally extended above the adjoining alveolar bone. Both protocols displayed β-TCP residues apparently undergoing resorption. Application of both materials appears safe, as they were associated with limited, if any, adverse events. Conclusion: rhGDF-5/β-TCP shows a significant potential to support/accelerate periodontal wound healing/regeneration. Application of rhGDF-5/β-TCP appears safe and should be further evaluated in human clinical trials.

Original language	English (US)
Pages (from-to)	739-746
Number of pages	8
Journal	Journal of Clinical Periodontology
Volume	37
Issue number	8
DOIs	https://doi.org/10.1111/j.1600-051X.2010.01576.x
State	Published - Aug 2010

Keywords

dog
periodontal regeneration
rhGDF-5
rhPDGF
tissue engineering
β-TCP

ASJC Scopus subject areas

Periodontics

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10.1111/j.1600-051X.2010.01576.x

Cite this

@article{f1f94f78bab24171be4913905e49a86b,

title = "Growth/differentiation factor-5 significantly enhances periodontal wound healing/regeneration compared with platelet-derived growth factor-BB in dogs",

abstract = "Objective: Recombinant human growth/differentiation factor-5 (rhGDF-5) in a particulate β-tricalcium phosphate (β-TCP) carrier is being evaluated to support periodontal regeneration. The objective of this study was to evaluate periodontal wound healing/regeneration following an established clinical (benchmark) protocol including surgical implantation of rhGDF-5/β-TCP in comparison with that following implantation of recombinant human platelet-derived growth factor-BB (rhPDGF) combined with a particulate β-TCP biomaterial using an established canine defect model. Materials and Methods: Bilateral, 4 × 5 mm (width × depth), one-wall, critical-size, intrabony periodontal defects were surgically created at the mandibular second and fourth pre-molar teeth in five adult Beagle dogs. Defect sites were randomized to receive rhGDF-5/β-TCP or the rhPDGF construct followed by wound closure for primary intention healing. The animals were sacrificed following an 8-week healing interval for histological and histometric examination. Results: Clinical healing was generally uneventful. Sites receiving rhGDF-5/β-TCP exhibited a significantly enhanced cementum formation compared with sites receiving the rhPDGF construct, averaging (±SD) 4.49±0.48 versus 2.72±0.91 mm (p<0.001). Similarly, bone regeneration height and area were significantly enhanced at sites receiving rhGDF-5/β-TCP versus that of the rhPDGF construct averaging, 3.08±0.74 versus 1.29±0.78 mm (p<0.001) and 6.03±1.28 versus 2.98±2.61 mm2 (p<0.01), respectively. Cementum regeneration included cellular/acellular mixed (extrinsic/intrinsic) fibre cementum at sites receiving rhGDF-5/β-TCP; sites receiving the rhPDGF/β-TCP showed a pre-dominantly acellular cementum. Newly formed cementum generally extended above the adjoining alveolar bone. Both protocols displayed β-TCP residues apparently undergoing resorption. Application of both materials appears safe, as they were associated with limited, if any, adverse events. Conclusion: rhGDF-5/β-TCP shows a significant potential to support/accelerate periodontal wound healing/regeneration. Application of rhGDF-5/β-TCP appears safe and should be further evaluated in human clinical trials.",

keywords = "dog, periodontal regeneration, rhGDF-5, rhPDGF, tissue engineering, β-TCP",

author = "Kwon, {Hyuk Rak} and Wikesj{\"o}, {Ulf M E} and Park, {Jung Chul} and Kim, {Young Taek} and Patrizia Bastone and Pippig, {Susanne D.} and Kim, {Chong Kwan}",

year = "2010",

month = aug,

doi = "10.1111/j.1600-051X.2010.01576.x",

language = "English (US)",

volume = "37",

pages = "739--746",

journal = "Journal of Clinical Periodontology",

issn = "0303-6979",

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T1 - Growth/differentiation factor-5 significantly enhances periodontal wound healing/regeneration compared with platelet-derived growth factor-BB in dogs

AU - Kwon, Hyuk Rak

AU - Wikesjö, Ulf M E

AU - Park, Jung Chul

AU - Kim, Young Taek

AU - Bastone, Patrizia

AU - Pippig, Susanne D.

AU - Kim, Chong Kwan

PY - 2010/8

Y1 - 2010/8

N2 - Objective: Recombinant human growth/differentiation factor-5 (rhGDF-5) in a particulate β-tricalcium phosphate (β-TCP) carrier is being evaluated to support periodontal regeneration. The objective of this study was to evaluate periodontal wound healing/regeneration following an established clinical (benchmark) protocol including surgical implantation of rhGDF-5/β-TCP in comparison with that following implantation of recombinant human platelet-derived growth factor-BB (rhPDGF) combined with a particulate β-TCP biomaterial using an established canine defect model. Materials and Methods: Bilateral, 4 × 5 mm (width × depth), one-wall, critical-size, intrabony periodontal defects were surgically created at the mandibular second and fourth pre-molar teeth in five adult Beagle dogs. Defect sites were randomized to receive rhGDF-5/β-TCP or the rhPDGF construct followed by wound closure for primary intention healing. The animals were sacrificed following an 8-week healing interval for histological and histometric examination. Results: Clinical healing was generally uneventful. Sites receiving rhGDF-5/β-TCP exhibited a significantly enhanced cementum formation compared with sites receiving the rhPDGF construct, averaging (±SD) 4.49±0.48 versus 2.72±0.91 mm (p<0.001). Similarly, bone regeneration height and area were significantly enhanced at sites receiving rhGDF-5/β-TCP versus that of the rhPDGF construct averaging, 3.08±0.74 versus 1.29±0.78 mm (p<0.001) and 6.03±1.28 versus 2.98±2.61 mm2 (p<0.01), respectively. Cementum regeneration included cellular/acellular mixed (extrinsic/intrinsic) fibre cementum at sites receiving rhGDF-5/β-TCP; sites receiving the rhPDGF/β-TCP showed a pre-dominantly acellular cementum. Newly formed cementum generally extended above the adjoining alveolar bone. Both protocols displayed β-TCP residues apparently undergoing resorption. Application of both materials appears safe, as they were associated with limited, if any, adverse events. Conclusion: rhGDF-5/β-TCP shows a significant potential to support/accelerate periodontal wound healing/regeneration. Application of rhGDF-5/β-TCP appears safe and should be further evaluated in human clinical trials.

AB - Objective: Recombinant human growth/differentiation factor-5 (rhGDF-5) in a particulate β-tricalcium phosphate (β-TCP) carrier is being evaluated to support periodontal regeneration. The objective of this study was to evaluate periodontal wound healing/regeneration following an established clinical (benchmark) protocol including surgical implantation of rhGDF-5/β-TCP in comparison with that following implantation of recombinant human platelet-derived growth factor-BB (rhPDGF) combined with a particulate β-TCP biomaterial using an established canine defect model. Materials and Methods: Bilateral, 4 × 5 mm (width × depth), one-wall, critical-size, intrabony periodontal defects were surgically created at the mandibular second and fourth pre-molar teeth in five adult Beagle dogs. Defect sites were randomized to receive rhGDF-5/β-TCP or the rhPDGF construct followed by wound closure for primary intention healing. The animals were sacrificed following an 8-week healing interval for histological and histometric examination. Results: Clinical healing was generally uneventful. Sites receiving rhGDF-5/β-TCP exhibited a significantly enhanced cementum formation compared with sites receiving the rhPDGF construct, averaging (±SD) 4.49±0.48 versus 2.72±0.91 mm (p<0.001). Similarly, bone regeneration height and area were significantly enhanced at sites receiving rhGDF-5/β-TCP versus that of the rhPDGF construct averaging, 3.08±0.74 versus 1.29±0.78 mm (p<0.001) and 6.03±1.28 versus 2.98±2.61 mm2 (p<0.01), respectively. Cementum regeneration included cellular/acellular mixed (extrinsic/intrinsic) fibre cementum at sites receiving rhGDF-5/β-TCP; sites receiving the rhPDGF/β-TCP showed a pre-dominantly acellular cementum. Newly formed cementum generally extended above the adjoining alveolar bone. Both protocols displayed β-TCP residues apparently undergoing resorption. Application of both materials appears safe, as they were associated with limited, if any, adverse events. Conclusion: rhGDF-5/β-TCP shows a significant potential to support/accelerate periodontal wound healing/regeneration. Application of rhGDF-5/β-TCP appears safe and should be further evaluated in human clinical trials.

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KW - periodontal regeneration

KW - rhGDF-5

KW - rhPDGF

KW - tissue engineering

KW - β-TCP

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Growth/differentiation factor-5 significantly enhances periodontal wound healing/regeneration compared with platelet-derived growth factor-BB in dogs

Abstract

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