Growth/differentiation factor-5 significantly enhances periodontal wound healing/regeneration compared with platelet-derived growth factor-BB in dogs

Hyuk Rak Kwon, Ulf M E Wikesjö, Jung Chul Park, Young Taek Kim, Patrizia Bastone, Susanne D. Pippig, Chong Kwan Kim

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Objective: Recombinant human growth/differentiation factor-5 (rhGDF-5) in a particulate β-tricalcium phosphate (β-TCP) carrier is being evaluated to support periodontal regeneration. The objective of this study was to evaluate periodontal wound healing/regeneration following an established clinical (benchmark) protocol including surgical implantation of rhGDF-5/β-TCP in comparison with that following implantation of recombinant human platelet-derived growth factor-BB (rhPDGF) combined with a particulate β-TCP biomaterial using an established canine defect model. Materials and Methods: Bilateral, 4 × 5 mm (width × depth), one-wall, critical-size, intrabony periodontal defects were surgically created at the mandibular second and fourth pre-molar teeth in five adult Beagle dogs. Defect sites were randomized to receive rhGDF-5/β-TCP or the rhPDGF construct followed by wound closure for primary intention healing. The animals were sacrificed following an 8-week healing interval for histological and histometric examination. Results: Clinical healing was generally uneventful. Sites receiving rhGDF-5/β-TCP exhibited a significantly enhanced cementum formation compared with sites receiving the rhPDGF construct, averaging (±SD) 4.49±0.48 versus 2.72±0.91 mm (p<0.001). Similarly, bone regeneration height and area were significantly enhanced at sites receiving rhGDF-5/β-TCP versus that of the rhPDGF construct averaging, 3.08±0.74 versus 1.29±0.78 mm (p<0.001) and 6.03±1.28 versus 2.98±2.61 mm2 (p<0.01), respectively. Cementum regeneration included cellular/acellular mixed (extrinsic/intrinsic) fibre cementum at sites receiving rhGDF-5/β-TCP; sites receiving the rhPDGF/β-TCP showed a pre-dominantly acellular cementum. Newly formed cementum generally extended above the adjoining alveolar bone. Both protocols displayed β-TCP residues apparently undergoing resorption. Application of both materials appears safe, as they were associated with limited, if any, adverse events. Conclusion: rhGDF-5/β-TCP shows a significant potential to support/accelerate periodontal wound healing/regeneration. Application of rhGDF-5/β-TCP appears safe and should be further evaluated in human clinical trials.

Original languageEnglish (US)
Pages (from-to)739-746
Number of pages8
JournalJournal of Clinical Periodontology
Volume37
Issue number8
DOIs
StatePublished - Aug 1 2010

Fingerprint

Growth Differentiation Factor 5
Wound Healing
Regeneration
Dental Cementum
Dogs
Phosphate Transport Proteins
Supernumerary Tooth
Benchmarking
Bone Regeneration
human GDF5 protein
platelet-derived growth factor BB
Biocompatible Materials
Clinical Protocols
Canidae
Tooth
Clinical Trials

Keywords

  • dog
  • periodontal regeneration
  • rhGDF-5
  • rhPDGF
  • tissue engineering
  • β-TCP

ASJC Scopus subject areas

  • Periodontics

Cite this

Growth/differentiation factor-5 significantly enhances periodontal wound healing/regeneration compared with platelet-derived growth factor-BB in dogs. / Kwon, Hyuk Rak; Wikesjö, Ulf M E; Park, Jung Chul; Kim, Young Taek; Bastone, Patrizia; Pippig, Susanne D.; Kim, Chong Kwan.

In: Journal of Clinical Periodontology, Vol. 37, No. 8, 01.08.2010, p. 739-746.

Research output: Contribution to journalArticle

Kwon, Hyuk Rak ; Wikesjö, Ulf M E ; Park, Jung Chul ; Kim, Young Taek ; Bastone, Patrizia ; Pippig, Susanne D. ; Kim, Chong Kwan. / Growth/differentiation factor-5 significantly enhances periodontal wound healing/regeneration compared with platelet-derived growth factor-BB in dogs. In: Journal of Clinical Periodontology. 2010 ; Vol. 37, No. 8. pp. 739-746.
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AU - Park, Jung Chul

AU - Kim, Young Taek

AU - Bastone, Patrizia

AU - Pippig, Susanne D.

AU - Kim, Chong Kwan

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