Gut pathology and responses to the microsporidium Nosema ceranae in the honey bee Apis mellifera

Claudia Dussaubat, Jean Luc Brunet, Mariano Higes, John K. Colbourne, Jacqueline Lopez, Jeong-Hyeon Choi, Raquel Martín-Hernández, Cristina Botías, Marianne Cousin, Cynthia McDonnell, Marc Bonnet, Luc P. Belzunces, Robin F.A. Moritz, Yves Le Conte, Cédric Alaux

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

The microsporidium Nosema ceranae is a newly prevalent parasite of the European honey bee (Apis mellifera). Although this parasite is presently spreading across the world into its novel host, the mechanisms by it which affects the bees and how bees respond are not well understood. We therefore performed an extensive characterization of the parasite effects at the molecular level by using genetic and biochemical tools. The transcriptome modifications at the midgut level were characterized seven days post-infection with tiling microarrays. Then we tested the bee midgut response to infection by measuring activity of antioxidant and detoxification enzymes (superoxide dismutases, glutathione peroxidases, glutathione reductase, and glutathione-S-transferase). At the gene-expression level, the bee midgut responded to N. ceranae infection by an increase in oxidative stress concurrent with the generation of antioxidant enzymes, defense and protective response specifically observed in the gut of mammals and insects. However, at the enzymatic level, the protective response was not confirmed, with only glutathione-S-transferase exhibiting a higher activity in infected bees. The oxidative stress was associated with a higher transcription of sugar transporter in the gut. Finally, a dramatic effect of the microsporidia infection was the inhibition of genes involved in the homeostasis and renewal of intestinal tissues (Wnt signaling pathway), a phenomenon that was confirmed at the histological level. This tissue degeneration and prevention of gut epithelium renewal may explain early bee death. In conclusion, our integrated approach not only gives new insights into the pathological effects of N. ceranae and the bee gut response, but also demonstrate that the honey bee gut is an interesting model system for studying host defense responses.

Original languageEnglish (US)
Article numbere37017
JournalPloS one
Volume7
Issue number5
DOIs
StatePublished - May 18 2012
Externally publishedYes

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Unclassified Microsporidia
Nosema
Nosema ceranae
Honey
Bees
Pathology
Apis mellifera
honey bees
Apoidea
Oxidative stress
digestive system
Glutathione Transferase
Antioxidants
Tissue
Detoxification
midgut
Mammals
Glutathione Reductase
Enzymes
Transcription

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Dussaubat, C., Brunet, J. L., Higes, M., Colbourne, J. K., Lopez, J., Choi, J-H., ... Alaux, C. (2012). Gut pathology and responses to the microsporidium Nosema ceranae in the honey bee Apis mellifera. PloS one, 7(5), [e37017]. https://doi.org/10.1371/journal.pone.0037017

Gut pathology and responses to the microsporidium Nosema ceranae in the honey bee Apis mellifera. / Dussaubat, Claudia; Brunet, Jean Luc; Higes, Mariano; Colbourne, John K.; Lopez, Jacqueline; Choi, Jeong-Hyeon; Martín-Hernández, Raquel; Botías, Cristina; Cousin, Marianne; McDonnell, Cynthia; Bonnet, Marc; Belzunces, Luc P.; Moritz, Robin F.A.; Le Conte, Yves; Alaux, Cédric.

In: PloS one, Vol. 7, No. 5, e37017, 18.05.2012.

Research output: Contribution to journalArticle

Dussaubat, C, Brunet, JL, Higes, M, Colbourne, JK, Lopez, J, Choi, J-H, Martín-Hernández, R, Botías, C, Cousin, M, McDonnell, C, Bonnet, M, Belzunces, LP, Moritz, RFA, Le Conte, Y & Alaux, C 2012, 'Gut pathology and responses to the microsporidium Nosema ceranae in the honey bee Apis mellifera', PloS one, vol. 7, no. 5, e37017. https://doi.org/10.1371/journal.pone.0037017
Dussaubat, Claudia ; Brunet, Jean Luc ; Higes, Mariano ; Colbourne, John K. ; Lopez, Jacqueline ; Choi, Jeong-Hyeon ; Martín-Hernández, Raquel ; Botías, Cristina ; Cousin, Marianne ; McDonnell, Cynthia ; Bonnet, Marc ; Belzunces, Luc P. ; Moritz, Robin F.A. ; Le Conte, Yves ; Alaux, Cédric. / Gut pathology and responses to the microsporidium Nosema ceranae in the honey bee Apis mellifera. In: PloS one. 2012 ; Vol. 7, No. 5.
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