Harvesting, identification and barrier function of human lung microvascular endothelial cells

John D. Catravas, Connie Snead, Christiana Dimitropoulou, Albert S.Y. Chang, Rudolf Lucas, Alexander Dmitriyevich Verin, Stephen Matthew Black

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Endothelial barrier dysfunction is an important contributor to the pathogenesis of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). Even though approaches that target the prevention and repair of endothelial barrier dysfunction are clearly needed, our understanding of the molecular regulation of pulmonary microvascular endothelial permeability remains incomplete. Cultured pulmonary microvascular endothelial cells represent an attractive paradigm for the study of barrier function. Here, we describe a method for the harvest, identification and culture of human lung microvascular endothelial cells (HLMVEC). HLMVEC thus obtained, grow as a monolayer, exhibit contact inhibition and have the typical cobblestone appearance. They express endothelial proteins, such as von Willebrand factor and endothelial nitric oxide synthase and take up an acetylated LDL. Furthermore, HLMVEC respond predictably and with superior sensitivity to the barrier disruptive effects of Gram positive and Gram negative bacterial products, thrombin, vascular endothelial growth factor and microtubule disrupting agents. These HLMVEC present an in-house-derived alternative to commercially available human cells for the study of mechanisms contributing to ALI and ARDS.

Original languageEnglish (US)
Pages (from-to)175-181
Number of pages7
JournalVascular Pharmacology
Volume52
Issue number5-6
DOIs
StatePublished - May 1 2010

Fingerprint

Endothelial Cells
Lung
Acute Lung Injury
Adult Respiratory Distress Syndrome
Contact Inhibition
Forensic Anthropology
Nitric Oxide Synthase Type III
von Willebrand Factor
Capillary Permeability
Thrombin
Microtubules
Vascular Endothelial Growth Factor A
Proteins

Keywords

  • Barrier function
  • Harvesting
  • Human
  • Permeability
  • Pulmonary endothelium

ASJC Scopus subject areas

  • Physiology
  • Molecular Medicine
  • Pharmacology

Cite this

Harvesting, identification and barrier function of human lung microvascular endothelial cells. / Catravas, John D.; Snead, Connie; Dimitropoulou, Christiana; Chang, Albert S.Y.; Lucas, Rudolf; Verin, Alexander Dmitriyevich; Black, Stephen Matthew.

In: Vascular Pharmacology, Vol. 52, No. 5-6, 01.05.2010, p. 175-181.

Research output: Contribution to journalArticle

Catravas, John D. ; Snead, Connie ; Dimitropoulou, Christiana ; Chang, Albert S.Y. ; Lucas, Rudolf ; Verin, Alexander Dmitriyevich ; Black, Stephen Matthew. / Harvesting, identification and barrier function of human lung microvascular endothelial cells. In: Vascular Pharmacology. 2010 ; Vol. 52, No. 5-6. pp. 175-181.
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