Hb Natal or α2(minus Tyr-Arg)β2: A high oxygen affinity α chain variant with a deleted carboxy-terminus resulting from a TAC → TAA (Tyr → terminating codon) mutation in codon α140

V. B. Jogessar, K. Westermeyer, B. B. Webber, J. B. Wilson, H. Hu, J. M. Gonzalez-Redondo, Abdullah Kutlar, T. H J Huisman

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The discovery is reported of a fast-moving α chain variant (Hb Natal) which is characterized by a shortened α polypeptide chain because of the deletion of the Tyr-Arg carboxy-terminal residues. Through amplification of appropriate segments of DNA and hybridization with synthetic oligonucleotide probes, it was possible to detect a C → A mutation in codon 140 of the α2 globin gene, which causes a change in the codon for tyrosine to a terminating codon. Hb Natal or α2(minus Tyr-Arg)β2 has a high affinity for oxygen without a Bohr effect and heme-heme interaction. These results provide direct evidence for the importance of the tyrosine residue at α140 in the oxygenation-deoxygenation process.

Original languageEnglish (US)
Pages (from-to)36-41
Number of pages6
JournalBBA - Gene Structure and Expression
Volume951
Issue number1
DOIs
StatePublished - Nov 10 1988

Fingerprint

kyotorphin
Heme
Codon
Tyrosine
Oxygen
Mutation
Oxygenation
Globins
Oligonucleotide Probes
Amplification
Genes
Peptides
DNA
hemoglobin Natal

Keywords

  • Gene amplification
  • HPLC
  • Mutation
  • Synthetic oligonucleotide

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

Cite this

Hb Natal or α2(minus Tyr-Arg)β2 : A high oxygen affinity α chain variant with a deleted carboxy-terminus resulting from a TAC → TAA (Tyr → terminating codon) mutation in codon α140. / Jogessar, V. B.; Westermeyer, K.; Webber, B. B.; Wilson, J. B.; Hu, H.; Gonzalez-Redondo, J. M.; Kutlar, Abdullah; Huisman, T. H J.

In: BBA - Gene Structure and Expression, Vol. 951, No. 1, 10.11.1988, p. 36-41.

Research output: Contribution to journalArticle

Jogessar, V. B. ; Westermeyer, K. ; Webber, B. B. ; Wilson, J. B. ; Hu, H. ; Gonzalez-Redondo, J. M. ; Kutlar, Abdullah ; Huisman, T. H J. / Hb Natal or α2(minus Tyr-Arg)β2 : A high oxygen affinity α chain variant with a deleted carboxy-terminus resulting from a TAC → TAA (Tyr → terminating codon) mutation in codon α140. In: BBA - Gene Structure and Expression. 1988 ; Vol. 951, No. 1. pp. 36-41.
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abstract = "The discovery is reported of a fast-moving α chain variant (Hb Natal) which is characterized by a shortened α polypeptide chain because of the deletion of the Tyr-Arg carboxy-terminal residues. Through amplification of appropriate segments of DNA and hybridization with synthetic oligonucleotide probes, it was possible to detect a C → A mutation in codon 140 of the α2 globin gene, which causes a change in the codon for tyrosine to a terminating codon. Hb Natal or α2(minus Tyr-Arg)β2 has a high affinity for oxygen without a Bohr effect and heme-heme interaction. These results provide direct evidence for the importance of the tyrosine residue at α140 in the oxygenation-deoxygenation process.",
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T2 - A high oxygen affinity α chain variant with a deleted carboxy-terminus resulting from a TAC → TAA (Tyr → terminating codon) mutation in codon α140

AU - Jogessar, V. B.

AU - Westermeyer, K.

AU - Webber, B. B.

AU - Wilson, J. B.

AU - Hu, H.

AU - Gonzalez-Redondo, J. M.

AU - Kutlar, Abdullah

AU - Huisman, T. H J

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AB - The discovery is reported of a fast-moving α chain variant (Hb Natal) which is characterized by a shortened α polypeptide chain because of the deletion of the Tyr-Arg carboxy-terminal residues. Through amplification of appropriate segments of DNA and hybridization with synthetic oligonucleotide probes, it was possible to detect a C → A mutation in codon 140 of the α2 globin gene, which causes a change in the codon for tyrosine to a terminating codon. Hb Natal or α2(minus Tyr-Arg)β2 has a high affinity for oxygen without a Bohr effect and heme-heme interaction. These results provide direct evidence for the importance of the tyrosine residue at α140 in the oxygenation-deoxygenation process.

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KW - Mutation

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