Healing patterns in calvarial bone defects following guided bone regeneration in rats

A micro-CT scan analysis

Carlalberta Verna, Carles Bosch, Michel Dalstra, Ulf M E Wikesjö, Leonardo Trombelli

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Objective: The objective of this study was to evaluate healing patterns of critical-size calvarial bone defects treated according to principles of guided bone regeneration using micro-CT scan analysis. Specifically, the contribution of bone, periosteum and dura mater to the amount and mineralization of newly formed bone was evaluated. Material and Methods: Surgically induced, critical-size calvarial bone defects in 48 adult male Wistar rats received the following: an occlusive expanded polytetrafluoroethylene (ePTFE) membrane at the exo- and endocranial aspect (OO; n = 12); an occlusive membrane at the exocranial and a perforated membrane at the endocranial aspect (OP; n = 12); a perforated membrane at the exocranial and an occlusive membrane at the endocranial aspect (PO; n = 12); and a perforated membrane at the exo- and endocranial aspect (PP; n = 12). The animals were euthanized at 4 weeks for quantitative analysis of bone volume fraction and mineralization in the region of interest (ROI) as well as in the external, middle and central area of the defect using micro-CT. Results: Bone volume fraction ranged from 31.4% (OP) to 24.5% (PP). No differences were found among the groups. Bone volume fraction and mineralization in the middle area were significantly greater in group OP than in group PP, and in the central area in group OO and PO than in group PP. Conclusions: The results of this study suggest that use of occlusive ePTFE membranes enhances bone formation and maturation in the calvarial skeleton. When occlusion of endo- and exocranial tissues was compromised by membrane perforation, impaired bone formation and mineralization were observed.

Original languageEnglish (US)
Pages (from-to)865-870
Number of pages6
JournalJournal of Clinical Periodontology
Volume29
Issue number9
DOIs
StatePublished - Sep 1 2002

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Bone Regeneration
Bone and Bones
Membranes
Polytetrafluoroethylene
Osteogenesis
Physiologic Calcification
Dura Mater
Periosteum
Skeleton
Wistar Rats

Keywords

  • Bone
  • Guided bone regeneration (GBR)
  • Micro-CT
  • Rats

ASJC Scopus subject areas

  • Periodontics

Cite this

Healing patterns in calvarial bone defects following guided bone regeneration in rats : A micro-CT scan analysis. / Verna, Carlalberta; Bosch, Carles; Dalstra, Michel; Wikesjö, Ulf M E; Trombelli, Leonardo.

In: Journal of Clinical Periodontology, Vol. 29, No. 9, 01.09.2002, p. 865-870.

Research output: Contribution to journalArticle

Verna, Carlalberta ; Bosch, Carles ; Dalstra, Michel ; Wikesjö, Ulf M E ; Trombelli, Leonardo. / Healing patterns in calvarial bone defects following guided bone regeneration in rats : A micro-CT scan analysis. In: Journal of Clinical Periodontology. 2002 ; Vol. 29, No. 9. pp. 865-870.
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abstract = "Objective: The objective of this study was to evaluate healing patterns of critical-size calvarial bone defects treated according to principles of guided bone regeneration using micro-CT scan analysis. Specifically, the contribution of bone, periosteum and dura mater to the amount and mineralization of newly formed bone was evaluated. Material and Methods: Surgically induced, critical-size calvarial bone defects in 48 adult male Wistar rats received the following: an occlusive expanded polytetrafluoroethylene (ePTFE) membrane at the exo- and endocranial aspect (OO; n = 12); an occlusive membrane at the exocranial and a perforated membrane at the endocranial aspect (OP; n = 12); a perforated membrane at the exocranial and an occlusive membrane at the endocranial aspect (PO; n = 12); and a perforated membrane at the exo- and endocranial aspect (PP; n = 12). The animals were euthanized at 4 weeks for quantitative analysis of bone volume fraction and mineralization in the region of interest (ROI) as well as in the external, middle and central area of the defect using micro-CT. Results: Bone volume fraction ranged from 31.4{\%} (OP) to 24.5{\%} (PP). No differences were found among the groups. Bone volume fraction and mineralization in the middle area were significantly greater in group OP than in group PP, and in the central area in group OO and PO than in group PP. Conclusions: The results of this study suggest that use of occlusive ePTFE membranes enhances bone formation and maturation in the calvarial skeleton. When occlusion of endo- and exocranial tissues was compromised by membrane perforation, impaired bone formation and mineralization were observed.",
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AU - Trombelli, Leonardo

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AB - Objective: The objective of this study was to evaluate healing patterns of critical-size calvarial bone defects treated according to principles of guided bone regeneration using micro-CT scan analysis. Specifically, the contribution of bone, periosteum and dura mater to the amount and mineralization of newly formed bone was evaluated. Material and Methods: Surgically induced, critical-size calvarial bone defects in 48 adult male Wistar rats received the following: an occlusive expanded polytetrafluoroethylene (ePTFE) membrane at the exo- and endocranial aspect (OO; n = 12); an occlusive membrane at the exocranial and a perforated membrane at the endocranial aspect (OP; n = 12); a perforated membrane at the exocranial and an occlusive membrane at the endocranial aspect (PO; n = 12); and a perforated membrane at the exo- and endocranial aspect (PP; n = 12). The animals were euthanized at 4 weeks for quantitative analysis of bone volume fraction and mineralization in the region of interest (ROI) as well as in the external, middle and central area of the defect using micro-CT. Results: Bone volume fraction ranged from 31.4% (OP) to 24.5% (PP). No differences were found among the groups. Bone volume fraction and mineralization in the middle area were significantly greater in group OP than in group PP, and in the central area in group OO and PO than in group PP. Conclusions: The results of this study suggest that use of occlusive ePTFE membranes enhances bone formation and maturation in the calvarial skeleton. When occlusion of endo- and exocranial tissues was compromised by membrane perforation, impaired bone formation and mineralization were observed.

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