Heat-induced increases in endothelial NO synthase expression and activity and endothelial NO release

M. Brennan Harris, Michele A. Blackstone, Hong Ju, Virginia J. Venema, Richard C Venema

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Endothelial nitric oxide (NO) synthase (eNOS) is regulated by heat shock protein 90 (HSP90), a heat-inducible protein; however, the effect of heat shock on eNOS expression and eNO release is unknown. Bovine aortic endothelial cells were incubated for 1 h at 37°C, 42°C, or 45°C and cell lysates were evaluated with the use of Western blotting. We observed a 2.1 ± 0.1-fold increase in eNOS protein content, but no change in HSP90 content, HSP70 content, or HSP90/eNOS association, 24 h after heat shock at 42°C. We also observed a 7.7 ± 1.5-fold increase in HSP70 protein content, but did not observe a change in eNOS or HSP90 24 h after heat shock at 45°C. eNOS activity and maximal bradykinin-stimulated NO release was significantly increased 24 h after heat shock at 42°C. Heat shock in rats (core temperature: 42°C, 15 min) resulted in a significant increase in aortic eNOS, HSP90, and HSP70 protein content. The aorta from heat-shocked rats exhibited a decreased maximal contractile response to phenylephrine, which was abolished by preincubation with N G -nitro-L-arginine. We conclude that prior heat shock is a physical stimulus of increased eNOS expression and is associated with an increase in eNOS activity, agonist-stimulated NO release, and a decreased vasoconstrictor response.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume285
Issue number1 54-1
StatePublished - Jul 1 2003

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Nitric Oxide Synthase Type III
HSP90 Heat-Shock Proteins
Nitric Oxide
Hot Temperature
Shock
Proteins
Bradykinin
Phenylephrine
Vasoconstrictor Agents
Arginine
Aorta
Endothelial Cells
Western Blotting
Temperature

Keywords

  • Endothelium
  • Heat shock
  • Heat shock proteins
  • Rat

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Heat-induced increases in endothelial NO synthase expression and activity and endothelial NO release. / Harris, M. Brennan; Blackstone, Michele A.; Ju, Hong; Venema, Virginia J.; Venema, Richard C.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 285, No. 1 54-1, 01.07.2003.

Research output: Contribution to journalArticle

Harris, M. Brennan ; Blackstone, Michele A. ; Ju, Hong ; Venema, Virginia J. ; Venema, Richard C. / Heat-induced increases in endothelial NO synthase expression and activity and endothelial NO release. In: American Journal of Physiology - Heart and Circulatory Physiology. 2003 ; Vol. 285, No. 1 54-1.
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