Abstract
Hyperthermic sensitivity, kinetics of thermotolerance induction and decay, and profile of heat shock protein synthesis were studied in human granulocyte-macrophage progenitors and nucleated bone marrow cells, respectively. The D0 of the heat survival curves of human granulocyte-macrophage colony-forming cells at 43°C, 44°C, and 45°C were 23,12, and 5 min, respectively. The kinetics of thermotolerance induction was measured with a triggering dose of 44°C/20 min, 45oC/10 min, 41°C/2 h, and 42°C/1 and 4 h. The cells were then challenged with 44°C or 45°C after incubation for 2, 4,6,24, and 48 h at 37°C. In all cases, except for 41°C/2 h, thermotoler-ance was maximum at 2 to 6 h, began to decay at 24 h, and decayed completely by 48 h. At the 41°C/2-h triggering dose, the thermotolerance decayed completely by 24 h. The heat shock protein synthesis was measured after a triggering dose of 44°C/20 min or 45°C/10 min. Synthesis of Mr 70,000 and 87,000 heat shock proteins in the total nucleated bone marrow cells was evident at least for 8 h after the initial heating.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3630-3633 |
| Number of pages | 4 |
| Journal | Cancer Research |
| Volume | 48 |
| Issue number | 13 |
| State | Published - Jan 1 1988 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Oncology
- Cancer Research
Cite this
Heat Sensitivity, Thermotolerance, and Profile of Protein Synthesis of Human Bone Marrow Progenitors. / Mivechi, Nahid F.
In: Cancer Research, Vol. 48, No. 13, 01.01.1988, p. 3630-3633.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Heat Sensitivity, Thermotolerance, and Profile of Protein Synthesis of Human Bone Marrow Progenitors
AU - Mivechi, Nahid F
PY - 1988/1/1
Y1 - 1988/1/1
N2 - Hyperthermic sensitivity, kinetics of thermotolerance induction and decay, and profile of heat shock protein synthesis were studied in human granulocyte-macrophage progenitors and nucleated bone marrow cells, respectively. The D0 of the heat survival curves of human granulocyte-macrophage colony-forming cells at 43°C, 44°C, and 45°C were 23,12, and 5 min, respectively. The kinetics of thermotolerance induction was measured with a triggering dose of 44°C/20 min, 45oC/10 min, 41°C/2 h, and 42°C/1 and 4 h. The cells were then challenged with 44°C or 45°C after incubation for 2, 4,6,24, and 48 h at 37°C. In all cases, except for 41°C/2 h, thermotoler-ance was maximum at 2 to 6 h, began to decay at 24 h, and decayed completely by 48 h. At the 41°C/2-h triggering dose, the thermotolerance decayed completely by 24 h. The heat shock protein synthesis was measured after a triggering dose of 44°C/20 min or 45°C/10 min. Synthesis of Mr 70,000 and 87,000 heat shock proteins in the total nucleated bone marrow cells was evident at least for 8 h after the initial heating.
AB - Hyperthermic sensitivity, kinetics of thermotolerance induction and decay, and profile of heat shock protein synthesis were studied in human granulocyte-macrophage progenitors and nucleated bone marrow cells, respectively. The D0 of the heat survival curves of human granulocyte-macrophage colony-forming cells at 43°C, 44°C, and 45°C were 23,12, and 5 min, respectively. The kinetics of thermotolerance induction was measured with a triggering dose of 44°C/20 min, 45oC/10 min, 41°C/2 h, and 42°C/1 and 4 h. The cells were then challenged with 44°C or 45°C after incubation for 2, 4,6,24, and 48 h at 37°C. In all cases, except for 41°C/2 h, thermotoler-ance was maximum at 2 to 6 h, began to decay at 24 h, and decayed completely by 48 h. At the 41°C/2-h triggering dose, the thermotolerance decayed completely by 24 h. The heat shock protein synthesis was measured after a triggering dose of 44°C/20 min or 45°C/10 min. Synthesis of Mr 70,000 and 87,000 heat shock proteins in the total nucleated bone marrow cells was evident at least for 8 h after the initial heating.
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UR - http://www.scopus.com/inward/citedby.url?scp=0023711864&partnerID=8YFLogxK
M3 - Article
C2 - 3378207
AN - SCOPUS:0023711864
VL - 48
SP - 3630
EP - 3633
JO - Cancer Research
JF - Cancer Research
SN - 0008-5472
IS - 13
ER -