Hyperthermic sensitivity, kinetics of thermotolerance induction and decay, and profile of heat shock protein synthesis were studied in human granulocyte-macrophage progenitors and nucleated bone marrow cells, respectively. The D0 of the heat survival curves of human granulocyte-macrophage colony-forming cells at 43°C, 44°C, and 45°C were 23,12, and 5 min, respectively. The kinetics of thermotolerance induction was measured with a triggering dose of 44°C/20 min, 45oC/10 min, 41°C/2 h, and 42°C/1 and 4 h. The cells were then challenged with 44°C or 45°C after incubation for 2, 4,6,24, and 48 h at 37°C. In all cases, except for 41°C/2 h, thermotoler-ance was maximum at 2 to 6 h, began to decay at 24 h, and decayed completely by 48 h. At the 41°C/2-h triggering dose, the thermotolerance decayed completely by 24 h. The heat shock protein synthesis was measured after a triggering dose of 44°C/20 min or 45°C/10 min. Synthesis of Mr 70,000 and 87,000 heat shock proteins in the total nucleated bone marrow cells was evident at least for 8 h after the initial heating.
|Original language||English (US)|
|Number of pages||4|
|State||Published - 1988|
ASJC Scopus subject areas
- Cancer Research