Heat shock improves sca-1+ stem cell survival and directs ischemic cardiomyocytes toward a prosurvival phenotype via exosomal transfer: A critical role for HSF1/miR-34a/HSP70 pathway

Yuliang Feng, Wei Huang, Wei Meng, Anil G. Jegga, Yigang Wang, Wenfeng Cai, Ha Won Kim, Zeeshan Pasha, Zhili Wen, Fang Rao, Rohan M. Modi, Xiyong Yu, Muhammad Ashraf

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Stem cell-based therapy is a promising intervention for ischemic heart diseases. However, the functional integrity of stem cells is impaired in an ischemic environment. Here, we report a novel finding that heat shock significantly improves Sca-1+stem cell survival in an ischemic environment by the regulation of the triangle: heat shock factor 1 (HSF1), HSF1/miR-34a, and heat shock protein 70 (HSP70). Initially we prove that HSP70 is the key chaperone-mediating cytoprotective effect of heat shock in Sca-1 +cells and then we establish miR-34a as a direct repressor of HSP70. We found that HSP70 was downregulated in heat shocked Sca-1+ stem cells (HSSca-1+ cells). Intriguingly, we demonstrate that the downregulation of miR-34a is attributed to HSF1-mediated epigenetic repression through histone H3 Lys27 trimethylation (H3K27me3) on miR-34a promoter. Moreover, we show that heat shock induces exosomal transfer of HSF1 from Sca-1+ cells, which directs ischemic cardiomyocytes toward a prosurvival phenotype by epigenetic repression of miR-34a. In addition, our in vivo study demonstrates that transplantation of HSSca-1+ cells significantly reduces apoptosis, attenuates fibrosis, and improves global heart functions in ischemic myocardium. Hence, our study provides not only novel insights into the effects of heat shock on stem cell survival and paracrine behavior but also may have therapeutic values for stem cell therapy in ischemic heart diseases.

Original languageEnglish (US)
Pages (from-to)462-472
Number of pages11
JournalStem Cells
Volume32
Issue number2
DOIs
StatePublished - Jan 1 2014

Fingerprint

HSP70 Heat-Shock Proteins
Cardiac Myocytes
Shock
Cell Survival
Stem Cells
Hot Temperature
Phenotype
Epigenetic Repression
Cell- and Tissue-Based Therapy
Myocardial Ischemia
Down-Regulation
Histones
Myocardium
Fibrosis
Transplantation
Apoptosis

Keywords

  • Cardiac
  • Chaperone
  • Exosome
  • Histone modification
  • Hypoxia
  • MiR-34a
  • Sca-1
  • Stem cell transplantation

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Cite this

Heat shock improves sca-1+ stem cell survival and directs ischemic cardiomyocytes toward a prosurvival phenotype via exosomal transfer : A critical role for HSF1/miR-34a/HSP70 pathway. / Feng, Yuliang; Huang, Wei; Meng, Wei; Jegga, Anil G.; Wang, Yigang; Cai, Wenfeng; Kim, Ha Won; Pasha, Zeeshan; Wen, Zhili; Rao, Fang; Modi, Rohan M.; Yu, Xiyong; Ashraf, Muhammad.

In: Stem Cells, Vol. 32, No. 2, 01.01.2014, p. 462-472.

Research output: Contribution to journalArticle

Feng, Yuliang ; Huang, Wei ; Meng, Wei ; Jegga, Anil G. ; Wang, Yigang ; Cai, Wenfeng ; Kim, Ha Won ; Pasha, Zeeshan ; Wen, Zhili ; Rao, Fang ; Modi, Rohan M. ; Yu, Xiyong ; Ashraf, Muhammad. / Heat shock improves sca-1+ stem cell survival and directs ischemic cardiomyocytes toward a prosurvival phenotype via exosomal transfer : A critical role for HSF1/miR-34a/HSP70 pathway. In: Stem Cells. 2014 ; Vol. 32, No. 2. pp. 462-472.
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AU - Meng, Wei

AU - Jegga, Anil G.

AU - Wang, Yigang

AU - Cai, Wenfeng

AU - Kim, Ha Won

AU - Pasha, Zeeshan

AU - Wen, Zhili

AU - Rao, Fang

AU - Modi, Rohan M.

AU - Yu, Xiyong

AU - Ashraf, Muhammad

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