Heat shock response in CHO mammalian cells is controlled by a nonlinear stochastic process

Ovidiu Lipan, Jean Marc Navenot, Zixuan Wang, Lei Huang, Stephen C. Peiper

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In many biological systems, the interactions that describe the coupling between different units in a genetic network are nonlinear and stochastic. We study the interplay between stochasticity and nonlinearity using the responses of Chinese hamster ovary (CHO) mammalian cells to different temperature shocks. The experimental data show that the mean value response of a cell population can be described by a mathematical expression (empirical law) which is valid for a large range of heat shock conditions. A nonlinear stochastic theoretical model was developed that explains the empirical law for the mean response. Moreover, the theoretical model predicts a specific biological probability distribution of responses for a cell population. The prediction was experimentally confirmed by measurements at the single-cell level. The computational approach can be used to study other nonlinear stochastic biological phenomena.

Original languageEnglish (US)
Pages (from-to)1859-1870
Number of pages12
JournalPLoS Computational Biology
Volume3
Issue number10
DOIs
StatePublished - Oct 1 2007

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Stochastic Processes
Heat-Shock Response
Ovary
stochastic processes
Nonlinear Process
heat shock
Chinese hamsters
stochasticity
Cricetulus
Random processes
Shock
Heat
Cells
Cell Population
Cell
Theoretical Model
nonlinearity
Nonlinear networks
Theoretical Models
cells

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Modeling and Simulation
  • Ecology
  • Molecular Biology
  • Genetics
  • Cellular and Molecular Neuroscience
  • Computational Theory and Mathematics

Cite this

Heat shock response in CHO mammalian cells is controlled by a nonlinear stochastic process. / Lipan, Ovidiu; Navenot, Jean Marc; Wang, Zixuan; Huang, Lei; Peiper, Stephen C.

In: PLoS Computational Biology, Vol. 3, No. 10, 01.10.2007, p. 1859-1870.

Research output: Contribution to journalArticle

Lipan, Ovidiu ; Navenot, Jean Marc ; Wang, Zixuan ; Huang, Lei ; Peiper, Stephen C. / Heat shock response in CHO mammalian cells is controlled by a nonlinear stochastic process. In: PLoS Computational Biology. 2007 ; Vol. 3, No. 10. pp. 1859-1870.
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