Helicobacter priori heat shock protein a: Serologic responses and genetic diversity

Enders K.W. Ng, Stuart A Thompson, Guillermo I. Pérez-Pérez, Imad Kansau, Arie Van Der Ende, Agnès Labigne, Joseph J.Y. Sung, S. C.Sydney Chung, Martin J. Blaser

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Helicobacter pylori synthesizes an unusual GroES homolog, heat shock protein A (HspA). The present study was aimed at an assessment of the serological response to HspA in a group of Chinese patients with defined gastroduodenal pathologies and determination of whether diversity is present in the nucleotide sequences encoding HspA in isolates from these patients. Serum samples collected from 154 patients who had an upper gastrointestinal pathology and the presence of H. pylori defined by biopsy were tested for an immunoglobulin G (IgG) serologic response to H. pylori HspA by an enzyme linked immunosorbant assay. HspA-encoding nucleotide sequences in H. pylori isolates from 14 patients (7 seropositive and 7 seronegative for HspA) were analyzed by PCR and direct sequencing of the PCR products. The sequencing results were compared to those of 48 isolates from other parts of the world. Of the 154 known H. pylori-positive patients, 54 (35.1%) were seropositive for HspA. The A domain (GroES homology) of HspA was highly conserved in the 14 isolates tested. Although the B domain (metal-binding site unique to H. pylori) resembled that in the known major variant, particular amino acid substitutions allowed definition of an HspA variant associated with isolates from East Asia. There were no associations between patient characteristics and HspA seropositivity or amino acid sequences. We confirmed in this study that the clinical outcomes of H. pylori infection are not related to HspA antigenicity or to sequence variation. However, B-domain sequence variation may be a marker for the study of the genetic diversity of H. pylori strains of different geographic origins.

Original languageEnglish (US)
Pages (from-to)377-382
Number of pages6
JournalClinical and Diagnostic Laboratory Immunology
Volume6
Issue number3
StatePublished - May 1 1999

Fingerprint

Helicobacter
Staphylococcal Protein A
Heat-Shock Proteins
Helicobacter pylori
Pathology
Nucleotides
Amino Acids
Polymerase Chain Reaction
Far East
Biopsy
Helicobacter Infections
Amino Acid Substitution
Amino Acid Sequence
Shock
Assays
Substitution reactions
Immunoglobulin G
Hot Temperature
Metals
Binding Sites

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

Cite this

Ng, E. K. W., Thompson, S. A., Pérez-Pérez, G. I., Kansau, I., Van Der Ende, A., Labigne, A., ... Blaser, M. J. (1999). Helicobacter priori heat shock protein a: Serologic responses and genetic diversity. Clinical and Diagnostic Laboratory Immunology, 6(3), 377-382.

Helicobacter priori heat shock protein a : Serologic responses and genetic diversity. / Ng, Enders K.W.; Thompson, Stuart A; Pérez-Pérez, Guillermo I.; Kansau, Imad; Van Der Ende, Arie; Labigne, Agnès; Sung, Joseph J.Y.; Chung, S. C.Sydney; Blaser, Martin J.

In: Clinical and Diagnostic Laboratory Immunology, Vol. 6, No. 3, 01.05.1999, p. 377-382.

Research output: Contribution to journalArticle

Ng, EKW, Thompson, SA, Pérez-Pérez, GI, Kansau, I, Van Der Ende, A, Labigne, A, Sung, JJY, Chung, SCS & Blaser, MJ 1999, 'Helicobacter priori heat shock protein a: Serologic responses and genetic diversity', Clinical and Diagnostic Laboratory Immunology, vol. 6, no. 3, pp. 377-382.
Ng EKW, Thompson SA, Pérez-Pérez GI, Kansau I, Van Der Ende A, Labigne A et al. Helicobacter priori heat shock protein a: Serologic responses and genetic diversity. Clinical and Diagnostic Laboratory Immunology. 1999 May 1;6(3):377-382.
Ng, Enders K.W. ; Thompson, Stuart A ; Pérez-Pérez, Guillermo I. ; Kansau, Imad ; Van Der Ende, Arie ; Labigne, Agnès ; Sung, Joseph J.Y. ; Chung, S. C.Sydney ; Blaser, Martin J. / Helicobacter priori heat shock protein a : Serologic responses and genetic diversity. In: Clinical and Diagnostic Laboratory Immunology. 1999 ; Vol. 6, No. 3. pp. 377-382.
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