Helicobacter pylori disrupts host cell membranes, initiating a repair response and cell proliferation

Li Ling Lin, Hsuan Cheng Huang, Satoshi Ogihara, Jin Town Wang, Meng Chuan Wu, Paul L. McNeil, Chiung Nien Chen, Hsueh Fen Juan

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Helicobacter pylori (H. pylori), the human stomach pathogen, lives on the inner surface of the stomach and causes chronic gastritis, peptic ulcer, and gastric cancer. Plasma membrane repair response is a matter of life and death for human cells against physical and biological damage. We here test the hypothesis that H. pylori also causes plasma membrane disruption injury, and that not only a membrane repair response but also a cell proliferation response are thereby activated. Vacuolating cytotoxin A (VacA) and cytotoxin-associated gene A (CagA) have been considered to be major H. pylori virulence factors. Gastric cancer cells were infected with H. pylori wild type (vacA+/cagA+), single mutant (ΔvacA or ΔcagA) or double mutant (ΔvacA/ΔcagA) strains and plasma membrane disruption events and consequent activation of membrane repair components monitored. OPEN ACCESS H. pylori disrupts the host cell plasma membrane, allowing localized dye and extracellular Ca2+ influx. Ca2+-triggered members of the annexin family, A1 and A4, translocate, in response to injury, to the plasma membrane, and cell surface expression of an exocytotic maker of repair, LAMP-2, increases. Additional forms of plasma membrane disruption, unrelated to H. pylori exposure, also promote host cell proliferation. We propose that H. pylori activation of a plasma membrane repair is pro-proliferative. This study might therefore provide new insight into potential mechanisms of H. pylori-induced gastric carcinogenesis.

Original languageEnglish (US)
Pages (from-to)10176-10192
Number of pages17
JournalInternational journal of molecular sciences
Volume13
Issue number8
DOIs
Publication statusPublished - Aug 2012

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Keywords

  • Annexin A4
  • Cell proliferation
  • Helicobacter pylori
  • Plasma membrane repair

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Lin, L. L., Huang, H. C., Ogihara, S., Wang, J. T., Wu, M. C., McNeil, P. L., ... Juan, H. F. (2012). Helicobacter pylori disrupts host cell membranes, initiating a repair response and cell proliferation. International journal of molecular sciences, 13(8), 10176-10192. https://doi.org/10.3390/ijms130810176