Hematopoietic origin of microglial and perivascular cells in brain

David C. Hess, Takanori Abe, William D. Hill, Angeline Martin Studdard, Jo Carothers, Masahiro Masuya, Paul A. Fleming, Christopher J. Drake, Makio Ogawa

Research output: Contribution to journalArticle

215 Citations (Scopus)

Abstract

Background: Bone marrow (BM)-derived cells differentiate into a wide variety of cell types. BM contains a heterogeneous population of stem and progenitor cells including hematopoietic stem cells, marrow stromal cells, and perhaps other progenitor cells. To establish unequivocally the transdifferentiation capability of a hematopoietic cell to a nonhematopoietic cell (endothelial cells, neurons, and glial cells), it is imperative to demonstrate that a single cell or clone of that single cell (clonal analysis) differentiates into cells comprising vessels or other cells in the brain. Methods: We generated mice that exhibited a high level of hematopoietic reconstitution from a single enhanced green fluorescent protein (EGFP) stem cell. To achieve this, we combined FACS sorting and cell culture to generate a population of cells derived from a single hematopoietic stem cell (Lin -, CD34-, c-kit+, and Sca-1+). Clonal populations of cells were then transplanted into lethally irradiated recipient mice. After 3-4 months of engraftment, some mice underwent middle cerebral artery (MCA) suture occlusion. EGFP immunocytochemistry and dual labeling was performed with cell-specific markers on tissue from various time points. Results: In all transplanted mice, EGFP+ highly ramified cells were seen in the brain parenchyma. These cells stained with RCA120 lectin and had the characteristics of parenchymal microglial cells. In brains without infarction and in uninfarcted brain regions of mice that underwent MCA occlusion, there were many EGFP+ cells in a perivascular distribution, associated with both small and larger blood vessels. The cells were tightly apposed to the vessel wall and some had long processes that enveloped the endothelial cells. After MCA occlusion, there was an influx of EGFP expressing cells in the ischemic tissue that colocalized with the "neovascularization." These EGFP+ cells were wrapped around endothelial cells in an albuminal location and did not coexpress von Willebrand Factor or CD31. We detected rare dual-labeled EGFP and NeuN-expressing cells. We detected two staining patterns. The more frequent pattern was phagocytosis of NeuN cells by EGFP expressing cells. However, we also detected rarer cells where the EGFP and NeuN appeared to be colocalized by confocal microscopy. Conclusions: HSC differentiate into parenchymal microglial cells and perivascular cells in the brain. The numbers of these cells increase after cerebral ischemia. The HSC is therefore one source of parenchymal microglial cells and a source for perivascular cells. After a cerebral infarction, there are rare HSC-derived cells that stain with the neuronal marker, NeuN. However, the more common pattern appears to represent phagocytosis of damaged neurons by EGFP+ microglial cells.

Original languageEnglish (US)
Pages (from-to)134-144
Number of pages11
JournalExperimental Neurology
Volume186
Issue number2
DOIs
StatePublished - Apr 1 2004

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Brain
Middle Cerebral Artery Infarction
Stem Cells
Endothelial Cells
Hematopoietic Stem Cells
Bone Marrow
enhanced green fluorescent protein
Single-Cell Analysis
Population
Brain Infarction
Neurons
Cytophagocytosis
Cerebral Infarction
von Willebrand Factor
Stromal Cells
Brain Ischemia
Phagocytosis
Lectins
Confocal Microscopy
Neuroglia

Keywords

  • Cerebral ischemia
  • Hematopoietic stem cell
  • Microglial
  • Perivascular cell

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Cite this

Hess, D. C., Abe, T., Hill, W. D., Studdard, A. M., Carothers, J., Masuya, M., ... Ogawa, M. (2004). Hematopoietic origin of microglial and perivascular cells in brain. Experimental Neurology, 186(2), 134-144. https://doi.org/10.1016/j.expneurol.2003.11.005

Hematopoietic origin of microglial and perivascular cells in brain. / Hess, David C.; Abe, Takanori; Hill, William D.; Studdard, Angeline Martin; Carothers, Jo; Masuya, Masahiro; Fleming, Paul A.; Drake, Christopher J.; Ogawa, Makio.

In: Experimental Neurology, Vol. 186, No. 2, 01.04.2004, p. 134-144.

Research output: Contribution to journalArticle

Hess, DC, Abe, T, Hill, WD, Studdard, AM, Carothers, J, Masuya, M, Fleming, PA, Drake, CJ & Ogawa, M 2004, 'Hematopoietic origin of microglial and perivascular cells in brain', Experimental Neurology, vol. 186, no. 2, pp. 134-144. https://doi.org/10.1016/j.expneurol.2003.11.005
Hess, David C. ; Abe, Takanori ; Hill, William D. ; Studdard, Angeline Martin ; Carothers, Jo ; Masuya, Masahiro ; Fleming, Paul A. ; Drake, Christopher J. ; Ogawa, Makio. / Hematopoietic origin of microglial and perivascular cells in brain. In: Experimental Neurology. 2004 ; Vol. 186, No. 2. pp. 134-144.
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AU - Abe, Takanori

AU - Hill, William D.

AU - Studdard, Angeline Martin

AU - Carothers, Jo

AU - Masuya, Masahiro

AU - Fleming, Paul A.

AU - Drake, Christopher J.

AU - Ogawa, Makio

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N2 - Background: Bone marrow (BM)-derived cells differentiate into a wide variety of cell types. BM contains a heterogeneous population of stem and progenitor cells including hematopoietic stem cells, marrow stromal cells, and perhaps other progenitor cells. To establish unequivocally the transdifferentiation capability of a hematopoietic cell to a nonhematopoietic cell (endothelial cells, neurons, and glial cells), it is imperative to demonstrate that a single cell or clone of that single cell (clonal analysis) differentiates into cells comprising vessels or other cells in the brain. Methods: We generated mice that exhibited a high level of hematopoietic reconstitution from a single enhanced green fluorescent protein (EGFP) stem cell. To achieve this, we combined FACS sorting and cell culture to generate a population of cells derived from a single hematopoietic stem cell (Lin -, CD34-, c-kit+, and Sca-1+). Clonal populations of cells were then transplanted into lethally irradiated recipient mice. After 3-4 months of engraftment, some mice underwent middle cerebral artery (MCA) suture occlusion. EGFP immunocytochemistry and dual labeling was performed with cell-specific markers on tissue from various time points. Results: In all transplanted mice, EGFP+ highly ramified cells were seen in the brain parenchyma. These cells stained with RCA120 lectin and had the characteristics of parenchymal microglial cells. In brains without infarction and in uninfarcted brain regions of mice that underwent MCA occlusion, there were many EGFP+ cells in a perivascular distribution, associated with both small and larger blood vessels. The cells were tightly apposed to the vessel wall and some had long processes that enveloped the endothelial cells. After MCA occlusion, there was an influx of EGFP expressing cells in the ischemic tissue that colocalized with the "neovascularization." These EGFP+ cells were wrapped around endothelial cells in an albuminal location and did not coexpress von Willebrand Factor or CD31. We detected rare dual-labeled EGFP and NeuN-expressing cells. We detected two staining patterns. The more frequent pattern was phagocytosis of NeuN cells by EGFP expressing cells. However, we also detected rarer cells where the EGFP and NeuN appeared to be colocalized by confocal microscopy. Conclusions: HSC differentiate into parenchymal microglial cells and perivascular cells in the brain. The numbers of these cells increase after cerebral ischemia. The HSC is therefore one source of parenchymal microglial cells and a source for perivascular cells. After a cerebral infarction, there are rare HSC-derived cells that stain with the neuronal marker, NeuN. However, the more common pattern appears to represent phagocytosis of damaged neurons by EGFP+ microglial cells.

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KW - Cerebral ischemia

KW - Hematopoietic stem cell

KW - Microglial

KW - Perivascular cell

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