Heparin bonding increases patency of long microvascular prostheses

Edmond F Ritter, Mohammed M. Fata, Adam M. Rudner, Bruce Klitzman

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The high thrombogenicity of synthetic biomaterials has limited their use for reconstructive microsurgery. Prime factors in the thrombogenicity of synthetic materials in contact with blood include gas nuclei at the blood gas interface as well as the inherent thrombogenicity of the materials themselves. Expanded polytetrafluoroethylene (ePTFE) vascular prostheses were denucleated by placement in acetone and ethanol followed by degassed saline or by placement in degassed saline subjected to hydrostatic pressure. Heparinized grafts were prepared by coating with tridodecylmethylammonium chloride (TDMAC), followed by immersion in heparin. Grafts were installed to reconstruct the femoral artery (1 X 10 mm) or as renaliliac bypasses (1 x 50 mm) in rats. In the femoral artery reconstruction model, control grafts thrombosed within 10 minutes of implantation. All acetone denucleated femoral grafts remained patent for 60 minutes but were occluded at day 1. All pressure denucleated femoral grafts remained patent for 60 minutes, whereas six were patent at 1 month. In contrast, 11 of 15 heparinized femoral grafts were patent at 1 month. In the renal iliac bypass model, all control grafts were thrombosed within 10 minutes, whereas all heparin bonded grafts remained patent at 1 month. This finding confirms that removal of air from small diameter ePTFE grafts decreases acute thrombogenicity and that heparin bonding further decreases thrombogenicity, suggesting that clinically useful lengths of microvascular prostheses may be possible.

Original languageEnglish (US)
Pages (from-to)142-146
Number of pages5
JournalPlastic and Reconstructive Surgery
Volume101
Issue number1
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

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Prostheses and Implants
Heparin
Transplants
Thigh
Polytetrafluoroethylene
Femoral Artery
Acetone
Thrombosis
Gases
Blood Vessel Prosthesis
Hydrostatic Pressure
Microsurgery
Biocompatible Materials
Immersion
Ethanol
Air
Kidney
Pressure

ASJC Scopus subject areas

  • Surgery

Cite this

Heparin bonding increases patency of long microvascular prostheses. / Ritter, Edmond F; Fata, Mohammed M.; Rudner, Adam M.; Klitzman, Bruce.

In: Plastic and Reconstructive Surgery, Vol. 101, No. 1, 01.01.1998, p. 142-146.

Research output: Contribution to journalArticle

Ritter, Edmond F ; Fata, Mohammed M. ; Rudner, Adam M. ; Klitzman, Bruce. / Heparin bonding increases patency of long microvascular prostheses. In: Plastic and Reconstructive Surgery. 1998 ; Vol. 101, No. 1. pp. 142-146.
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