Hepatitis B virus-reactive human T-lymphocyte clones: Antigen specificity and helper function or antibody synthesis

Esteban Celis, P. C. Kung, T. W. Chang

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

To study immunity to hepatitis B surface antigen (HBsAg) at the cellular level, lymphocytes were obtained from the peripheral blood of hepatitis B vaccine recipients and were examined for various immune responses to HBsAg in vitro. The peripheral blood mononuclear cells (PBM) from most of the vaccinees did not proliferate to a great extent to HBsAg in vitro. However, HBsAg-reactive lymphocyte lines and clones were obtained from some of these individuals if the PBM were stimulated in vitro with HBsAg and were maintained in the presence of T cells growth supplement. Most of the HBsAg-reactive T cell clones obtained were found to be antigen-specific and some of them provided help in the production of anti-HBsAg antibodies by a cell population enriched for HBsAg-binding cells. These results indicate that HBsAg-specific T and B cells exist in the circulation of hepatitis B vaccine recipients, although they are at limiting concentrations for the in vitro cell proliferation and antibody production assays.

Original languageEnglish (US)
Pages (from-to)1511-1516
Number of pages6
JournalJournal of Immunology
Volume132
Issue number3
StatePublished - Jan 1 1984
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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