Hepatitis C virus nonstructural protein NS3 transforms NIH 3T3 cells

Daitoku Sakamuro, T. Furukawa, T. Takegami

Research output: Contribution to journalArticle

274 Citations (Scopus)

Abstract

Clinical evidence suggests that hepatitis C virus (HCV) is etiologically involved in hepatic cancer and river cirrhosis. To investigate whether the HCV nonstructural protein NS3 has oncogenic activity, NIH 3T3 cells were transfected with an expression vector containing cDNA for the 5'- or 3'-half sequence of the HCV genome segment encoding NS3. Only cells transfected with the 5'-half cDNA rapidly proliferated, lost contact inhibition, grew anchorage independently in soft agar, and formed tumors in nude mice. PCR analysis confirmed the presence of the 5'-half DNA in the transfectants. These results suggest that the 5' region of the HCV genome segment encoding NS3 is involved in cell transformation.

Original languageEnglish (US)
Pages (from-to)3893-3896
Number of pages4
JournalJournal of Virology
Volume69
Issue number6
StatePublished - Jan 1 1995
Externally publishedYes

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NIH 3T3 Cells
Hepatitis C virus
Hepacivirus
Complementary DNA
Genome
Contact Inhibition
proteins
cells
Liver Neoplasms
Rivers
Nude Mice
Agar
genome
liver neoplasms
Fibrosis
Polymerase Chain Reaction
agar
DNA
rivers
neoplasms

ASJC Scopus subject areas

  • Immunology

Cite this

Hepatitis C virus nonstructural protein NS3 transforms NIH 3T3 cells. / Sakamuro, Daitoku; Furukawa, T.; Takegami, T.

In: Journal of Virology, Vol. 69, No. 6, 01.01.1995, p. 3893-3896.

Research output: Contribution to journalArticle

Sakamuro, D, Furukawa, T & Takegami, T 1995, 'Hepatitis C virus nonstructural protein NS3 transforms NIH 3T3 cells', Journal of Virology, vol. 69, no. 6, pp. 3893-3896.
Sakamuro, Daitoku ; Furukawa, T. ; Takegami, T. / Hepatitis C virus nonstructural protein NS3 transforms NIH 3T3 cells. In: Journal of Virology. 1995 ; Vol. 69, No. 6. pp. 3893-3896.
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