TY - JOUR
T1 - Hepatoprotective activity of Feronia limonia root
AU - Jain, Mahendra
AU - Kapadia, Rakhee
AU - Jadeja, Ravirajsinh N.
AU - Thounaojam, Menaka C.
AU - Devkar, Ranjitsinh V.
AU - Mishra, Shri H.
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012/6
Y1 - 2012/6
N2 - Objectives The aim of this study was to evaluate the hepatoprotective potential of a methanolic extract and of marmesin isolated from the root bark of Feronia limonia. Methods Activity levels of aspartate aminotransaminase (AST) and alanine aminotransaminase (ALT), cell viability and cell death were evaluated in HepG2 cells (human liver hepatoma cells) treated with CCl 4 in the presence or absence of F. limonia extract or marmesin. Plasma activity levels of AST, ALT, bilirubin, alkaline phosphatase, protein, hepatic antioxidants, lipid peroxidation and histopathological evaluations were carried out in rats treated with CCl 4 alone or co-supplemented with F. limonia extract or marmesin in a dose-dependent manner. Key findings In-vitro co-supplementation of F. limonia methanolic extract or marmesin significantly minimized alteration in levels of AST and ALT and improved cell viability. Oral administration of F. limonia methanolic extract or marmesin significantly prevented CCl 4-induced elevation in the plasma markers of hepatic damage and hepatic lipid peroxidation and a decrease in hepatic antioxidants. In-vivo hepatoprotective potential of F. limonia methanolic extract and marmesin was evident from the minimal alterations in the histoarchitecture of liver. Conclusions This has been the first scientific report on the hepatoprotective potential of F. limonia root bark methanolic extract and marmesin.
AB - Objectives The aim of this study was to evaluate the hepatoprotective potential of a methanolic extract and of marmesin isolated from the root bark of Feronia limonia. Methods Activity levels of aspartate aminotransaminase (AST) and alanine aminotransaminase (ALT), cell viability and cell death were evaluated in HepG2 cells (human liver hepatoma cells) treated with CCl 4 in the presence or absence of F. limonia extract or marmesin. Plasma activity levels of AST, ALT, bilirubin, alkaline phosphatase, protein, hepatic antioxidants, lipid peroxidation and histopathological evaluations were carried out in rats treated with CCl 4 alone or co-supplemented with F. limonia extract or marmesin in a dose-dependent manner. Key findings In-vitro co-supplementation of F. limonia methanolic extract or marmesin significantly minimized alteration in levels of AST and ALT and improved cell viability. Oral administration of F. limonia methanolic extract or marmesin significantly prevented CCl 4-induced elevation in the plasma markers of hepatic damage and hepatic lipid peroxidation and a decrease in hepatic antioxidants. In-vivo hepatoprotective potential of F. limonia methanolic extract and marmesin was evident from the minimal alterations in the histoarchitecture of liver. Conclusions This has been the first scientific report on the hepatoprotective potential of F. limonia root bark methanolic extract and marmesin.
KW - Feronia limonia root bark
KW - HepG2 cells
KW - carbon tetrachloride
KW - marmesin
UR - http://www.scopus.com/inward/record.url?scp=84861098489&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84861098489&partnerID=8YFLogxK
U2 - 10.1111/j.2042-7158.2012.01481.x
DO - 10.1111/j.2042-7158.2012.01481.x
M3 - Article
C2 - 22571268
AN - SCOPUS:84861098489
SN - 0022-3573
VL - 64
SP - 888
EP - 896
JO - Journal of Pharmacy and Pharmacology
JF - Journal of Pharmacy and Pharmacology
IS - 6
ER -