hESC-derived Olig2+ progenitors generate a subtype of astroglia with protective effects against ischaemic brain injury

Peng Jiang, Chen Chen, Ruimin Wang, Olga V. Chechneva, Seung Hyuk Chung, Mahendra S. Rao, David E. Pleasure, Ying Liu, Quanguang Zhang, Wenbin Deng

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Human pluripotent stem cells (hPSCs) have been differentiated to astroglia, but the utilization of hPSC-derived astroglia as cell therapy for neurological diseases has not been well studied. Astroglia are heterogeneous, and not all astroglia are equivalent in promoting neural repair. A prerequisite for cell therapy is to derive defined cell populations with superior therapeutic effects. Here we use an Olig2-GFP human embryonic stem cell (hESC) reporter to demonstrate that hESC-derived Olig2+ progenitors generate a subtype of previously uncharacterized astroglia (Olig2PC-Astros). These Olig2PC-Astros differ substantially from astroglia differentiated from Olig2-negative hESC-derived neural progenitor cells (NPC-Astros), particularly in their neuroprotective properties. When grafted into brains subjected to global ischaemia, Olig2PC-Astros exhibit superior neuroprotective effects and improved behavioural outcome compared to NPC-Astros. Thus, this new paradigm of human astroglial differentiation is useful for studying the heterogeneity of human astroglia, and the unique Olig2PC-Astros may constitute a new cell therapy for treating cerebral ischaemia and other neurological diseases.

Original languageEnglish (US)
Article number2196
JournalNature communications
Volume4
DOIs
StatePublished - Dec 1 2013

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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    Jiang, P., Chen, C., Wang, R., Chechneva, O. V., Chung, S. H., Rao, M. S., Pleasure, D. E., Liu, Y., Zhang, Q., & Deng, W. (2013). hESC-derived Olig2+ progenitors generate a subtype of astroglia with protective effects against ischaemic brain injury. Nature communications, 4, [2196]. https://doi.org/10.1038/ncomms3196