Heterogeneity in presynaptic regulation of GABA release from hippocampal inhibitory neurons

Nevin A Lambert, Wilkie A. Wilson

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Release of GABA from the terminals of hippocampal inhibitory neurons is inhibited by activation of GABAB autoreceptors and μ opioid receptors. However, it is not known whether these presynaptic processes affect all inhibitory synapses equally. We examined the effects of the GABAB receptor agonist baclofen and the p opioid receptor agonist DAGO on postsynaptic currents evoked by minimal stimulation of inhibitory fibers (mePSCs) in area CA3. Baclofen reversibly depressed approximately half of the meIPSCs evoked in the stratum pyramidale. The remaining meIPSCs were unaffected despite a coincident depression of spontaneous IPSCs. In contrast, all meIPSCs were depressed by DAGO. In addition, minimal stimulation in the stratum radiatum evoked me1PSCs that were always depressed by baclofen. These results indicate that regulation of GABA release by GABAB autoreceptors occurs at a subset of inhibitory synapses and that GABAB-resistant inhibitory synapses are located on pyramidal neuron somata. Hippocampal inhibitory neurons may be heterogeneous with respect to presynaptic receptor-mediated regulation of GABA release.

Original languageEnglish (US)
Pages (from-to)1057-1067
Number of pages11
JournalNeuron
Volume11
Issue number6
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Synapses
Ala(2)-MePhe(4)-Gly(5)-enkephalin
gamma-Aminobutyric Acid
Autoreceptors
Baclofen
Opioid Receptors
Neurons
GABA-B Receptors
Presynaptic Receptors
Synaptic Potentials
Pyramidal Cells
Carisoprodol

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Heterogeneity in presynaptic regulation of GABA release from hippocampal inhibitory neurons. / Lambert, Nevin A; Wilson, Wilkie A.

In: Neuron, Vol. 11, No. 6, 01.01.1993, p. 1057-1067.

Research output: Contribution to journalArticle

@article{d32f29cbf7f44fa9bd5d97dfc6638b3d,
title = "Heterogeneity in presynaptic regulation of GABA release from hippocampal inhibitory neurons",
abstract = "Release of GABA from the terminals of hippocampal inhibitory neurons is inhibited by activation of GABAB autoreceptors and μ opioid receptors. However, it is not known whether these presynaptic processes affect all inhibitory synapses equally. We examined the effects of the GABAB receptor agonist baclofen and the p opioid receptor agonist DAGO on postsynaptic currents evoked by minimal stimulation of inhibitory fibers (mePSCs) in area CA3. Baclofen reversibly depressed approximately half of the meIPSCs evoked in the stratum pyramidale. The remaining meIPSCs were unaffected despite a coincident depression of spontaneous IPSCs. In contrast, all meIPSCs were depressed by DAGO. In addition, minimal stimulation in the stratum radiatum evoked me1PSCs that were always depressed by baclofen. These results indicate that regulation of GABA release by GABAB autoreceptors occurs at a subset of inhibitory synapses and that GABAB-resistant inhibitory synapses are located on pyramidal neuron somata. Hippocampal inhibitory neurons may be heterogeneous with respect to presynaptic receptor-mediated regulation of GABA release.",
author = "Lambert, {Nevin A} and Wilson, {Wilkie A.}",
year = "1993",
month = "1",
day = "1",
doi = "10.1016/0896-6273(93)90219-H",
language = "English (US)",
volume = "11",
pages = "1057--1067",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "6",

}

TY - JOUR

T1 - Heterogeneity in presynaptic regulation of GABA release from hippocampal inhibitory neurons

AU - Lambert, Nevin A

AU - Wilson, Wilkie A.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Release of GABA from the terminals of hippocampal inhibitory neurons is inhibited by activation of GABAB autoreceptors and μ opioid receptors. However, it is not known whether these presynaptic processes affect all inhibitory synapses equally. We examined the effects of the GABAB receptor agonist baclofen and the p opioid receptor agonist DAGO on postsynaptic currents evoked by minimal stimulation of inhibitory fibers (mePSCs) in area CA3. Baclofen reversibly depressed approximately half of the meIPSCs evoked in the stratum pyramidale. The remaining meIPSCs were unaffected despite a coincident depression of spontaneous IPSCs. In contrast, all meIPSCs were depressed by DAGO. In addition, minimal stimulation in the stratum radiatum evoked me1PSCs that were always depressed by baclofen. These results indicate that regulation of GABA release by GABAB autoreceptors occurs at a subset of inhibitory synapses and that GABAB-resistant inhibitory synapses are located on pyramidal neuron somata. Hippocampal inhibitory neurons may be heterogeneous with respect to presynaptic receptor-mediated regulation of GABA release.

AB - Release of GABA from the terminals of hippocampal inhibitory neurons is inhibited by activation of GABAB autoreceptors and μ opioid receptors. However, it is not known whether these presynaptic processes affect all inhibitory synapses equally. We examined the effects of the GABAB receptor agonist baclofen and the p opioid receptor agonist DAGO on postsynaptic currents evoked by minimal stimulation of inhibitory fibers (mePSCs) in area CA3. Baclofen reversibly depressed approximately half of the meIPSCs evoked in the stratum pyramidale. The remaining meIPSCs were unaffected despite a coincident depression of spontaneous IPSCs. In contrast, all meIPSCs were depressed by DAGO. In addition, minimal stimulation in the stratum radiatum evoked me1PSCs that were always depressed by baclofen. These results indicate that regulation of GABA release by GABAB autoreceptors occurs at a subset of inhibitory synapses and that GABAB-resistant inhibitory synapses are located on pyramidal neuron somata. Hippocampal inhibitory neurons may be heterogeneous with respect to presynaptic receptor-mediated regulation of GABA release.

UR - http://www.scopus.com/inward/record.url?scp=0027729948&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027729948&partnerID=8YFLogxK

U2 - 10.1016/0896-6273(93)90219-H

DO - 10.1016/0896-6273(93)90219-H

M3 - Article

C2 - 8274277

AN - SCOPUS:0027729948

VL - 11

SP - 1057

EP - 1067

JO - Neuron

JF - Neuron

SN - 0896-6273

IS - 6

ER -