Heterogeneity of cell response to early anoxia and reoxygenation in rat heart

P. M. Rahamathulla, M. Ashraf

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Heterogeneous cell injury and increased tissue calcium levels were observed in the isolated rat hearts perfused with anoxic medium for 5, 10, 15, 20, 25 and 30 min. These changes were intensified after reoxygenation of the anoxic hearts. The cell injury was quantitated using a score of 0, 1, 2 or 3 (0 being normal and 3 being severe injury). All four classes of cells were present even after 5 min of anoxia. The injury and calcium content increased with increase in duration of anoxia. Reduction in the percentage of injured cells was observed on reoxygenation of hearts perfused with anoxic medium for 5 to 20 min. However, reoxygenation of hearts initially perfused with anoxic medium for 25 to 30 min resulted in a higher percentage of injured myocytes. Beneficial effects of reoxygenation were observed in the anoxic hearts when the percentage of cells with an injury score of 1 and glycogen deposit were high, coupled with low calcium levels.

Original languageEnglish (US)
Pages (from-to)139-146
Number of pages8
JournalExperimental Pathology
Volume25
Issue number3
DOIs
StatePublished - Jan 1 1984
Externally publishedYes

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Keywords

  • anoxia
  • calcium content
  • cardiac muscle
  • cell response
  • electron microscopy
  • heart
  • heterogeneous cell population
  • reoxygenation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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