Heterogeneity of the 59-kDa dystrophin-associated protein revealed by cDNA cloning and expression

Bin Yang, Oxana Ibraghimov-Beskrovnaya, Carolyn R. Moomaw, Clive A. Slaughter, Kevin P. Campbell

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

The 59-kDa dystrophin-associated protein triplet (59-DAP) is a component of the dystrophin-glycoprotein complex which may directly associate with dystrophin. The cDNA encoding one component (59-1 DAP) of the 59-DAP triplet has now been cloned from rabbit skeletal muscle. The deduced amino acid sequence of 59-1 DAP predicts a 505-amino acid polypeptide containing nine potential phosphorylation sites and no predicted transmembrane domains. This is consistent with the 59-1 DAP being a peripheral membrane protein associated with the cytoplasmic face of the dystrophin-glycoprotein complex. Affinity-purified antibodies against rabbit 59-1 DAP fusion proteins only recognize the lowest band of the 59-DAP triplet in skeletal muscle sarcolemma and isolated dystrophin-glycoprotein complex. The tissue-specific expression of 59-1 DAP mRNA, which is most prominent in skeletal and cardiac muscle and is also detected in brain, parallels that of dystrophin but not of utrophin. Levels of 59-1 DAP mRNA are unaffected in mdx mouse skeletal and cardiac muscles, although all dystrophin-associated proteins, including 59-DAP, are greatly reduced in mdx mouse skeletal muscle. However, in mdx mouse cardiac muscle, the up-regulation of utrophin preserves all dystrophin-associated proteins except 59-DAP. Our results suggest that the 59-DAP triplet may contain different protein species and that the 59-1 DAP may associate more specifically with dystrophin than with utrophin.

Original languageEnglish (US)
Pages (from-to)6040-6044
Number of pages5
JournalJournal of Biological Chemistry
Volume269
Issue number8
StatePublished - Feb 25 1994
Externally publishedYes

Fingerprint

Dystrophin-Associated Proteins
Cloning
Dystrophin
Organism Cloning
Complementary DNA
Muscle
Utrophin
Inbred mdx Mouse
Skeletal Muscle
Myocardium
Glycoproteins
Rabbits
Amino Acids
Sarcolemma
Messenger RNA
Phosphorylation
Antibody Affinity
ARK 62-62
Amino Acid Sequence
Brain

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Yang, B., Ibraghimov-Beskrovnaya, O., Moomaw, C. R., Slaughter, C. A., & Campbell, K. P. (1994). Heterogeneity of the 59-kDa dystrophin-associated protein revealed by cDNA cloning and expression. Journal of Biological Chemistry, 269(8), 6040-6044.

Heterogeneity of the 59-kDa dystrophin-associated protein revealed by cDNA cloning and expression. / Yang, Bin; Ibraghimov-Beskrovnaya, Oxana; Moomaw, Carolyn R.; Slaughter, Clive A.; Campbell, Kevin P.

In: Journal of Biological Chemistry, Vol. 269, No. 8, 25.02.1994, p. 6040-6044.

Research output: Contribution to journalArticle

Yang, B, Ibraghimov-Beskrovnaya, O, Moomaw, CR, Slaughter, CA & Campbell, KP 1994, 'Heterogeneity of the 59-kDa dystrophin-associated protein revealed by cDNA cloning and expression', Journal of Biological Chemistry, vol. 269, no. 8, pp. 6040-6044.
Yang, Bin ; Ibraghimov-Beskrovnaya, Oxana ; Moomaw, Carolyn R. ; Slaughter, Clive A. ; Campbell, Kevin P. / Heterogeneity of the 59-kDa dystrophin-associated protein revealed by cDNA cloning and expression. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 8. pp. 6040-6044.
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