HIF-1α regulates function and differentiation of myeloid-derived suppressor cells in the tumor microenvironment

Cesar A. Corzo, Thomas Condamine, Lily Lu, Matthew J. Cotter, Je In Youn, Pingyan Cheng, Hyun Il Cho, Esteban Celis, David G. Quiceno, Tapan Padhya, Thomas V. McCaffrey, Judith C. McCaffrey, Dmitry I. Gabrilovich

Research output: Contribution to journalArticle

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Abstract

Myeloid-derived suppressor cells (MDSCs) are a major component of the immune-suppressive network described in cancer and many other pathological conditions. We demonstrate that although MDSCs from peripheral lymphoid organs and the tumor site share similar phenotype and morphology, these cells display profound functional differences. MDSC from peripheral lymphoid organs suppressed antigen-specific CD8 + T cells but failed to inhibit nonspecific T cell function. In sharp contrast, tumor MDSC suppressed both antigen-specific and nonspecific T cell activity. The tumor microenvironment caused rapid and dramatic up-regulation of arginase I and inducible nitric oxide synthase in MDSC, which was accompanied by down-regulation of nicotinamide adenine dinucleotide phosphate-oxidase and reactive oxygen species in these cells. In contrast to MDSC from the spleen, MDSC from the tumor site rapidly differentiated into macrophages. Exposure of spleen MDSC to hypoxia resulted in the conversion of these cells to nonspecific suppressors and their preferential differentiation to macrophages. Hypoxia-inducible factor (HIF) 1α was found to be primarily responsible for the observed effects of the tumor microenvironment on MDSC differentiation and function. Thus, hypoxia via HIF-1α dramatically alters the function of MDSC in the tumor microenvironment and redirects their differentiation toward tumor-associated macrophages, hence providing a mechanistic link between different myeloid suppressive cells in the tumor microenvironment.

Original languageEnglish (US)
Pages (from-to)2439-2453
Number of pages15
JournalJournal of Experimental Medicine
Volume207
Issue number11
DOIs
StatePublished - Oct 25 2010
Externally publishedYes

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Hypoxia-Inducible Factor 1
Tumor Microenvironment
Macrophages
Neoplasms
T-Lymphocytes
Spleen
Myeloid-Derived Suppressor Cells
CD8 Antigens
Arginase
Cell Hypoxia
Nitric Oxide Synthase Type II
Myeloid Cells
NADP
Cell Differentiation
Reactive Oxygen Species
Oxidoreductases
Up-Regulation
Down-Regulation
Phenotype
Antigens

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Corzo, C. A., Condamine, T., Lu, L., Cotter, M. J., Youn, J. I., Cheng, P., ... Gabrilovich, D. I. (2010). HIF-1α regulates function and differentiation of myeloid-derived suppressor cells in the tumor microenvironment. Journal of Experimental Medicine, 207(11), 2439-2453. https://doi.org/10.1084/jem.20100587

HIF-1α regulates function and differentiation of myeloid-derived suppressor cells in the tumor microenvironment. / Corzo, Cesar A.; Condamine, Thomas; Lu, Lily; Cotter, Matthew J.; Youn, Je In; Cheng, Pingyan; Cho, Hyun Il; Celis, Esteban; Quiceno, David G.; Padhya, Tapan; McCaffrey, Thomas V.; McCaffrey, Judith C.; Gabrilovich, Dmitry I.

In: Journal of Experimental Medicine, Vol. 207, No. 11, 25.10.2010, p. 2439-2453.

Research output: Contribution to journalArticle

Corzo, CA, Condamine, T, Lu, L, Cotter, MJ, Youn, JI, Cheng, P, Cho, HI, Celis, E, Quiceno, DG, Padhya, T, McCaffrey, TV, McCaffrey, JC & Gabrilovich, DI 2010, 'HIF-1α regulates function and differentiation of myeloid-derived suppressor cells in the tumor microenvironment', Journal of Experimental Medicine, vol. 207, no. 11, pp. 2439-2453. https://doi.org/10.1084/jem.20100587
Corzo, Cesar A. ; Condamine, Thomas ; Lu, Lily ; Cotter, Matthew J. ; Youn, Je In ; Cheng, Pingyan ; Cho, Hyun Il ; Celis, Esteban ; Quiceno, David G. ; Padhya, Tapan ; McCaffrey, Thomas V. ; McCaffrey, Judith C. ; Gabrilovich, Dmitry I. / HIF-1α regulates function and differentiation of myeloid-derived suppressor cells in the tumor microenvironment. In: Journal of Experimental Medicine. 2010 ; Vol. 207, No. 11. pp. 2439-2453.
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