High affinity of anti-GBM antibodies from goodpasture and transplanted Alport patients to α3(IV)NC1 collagen

Abraham Rutgers, Kevin E.C. Meyers, Gabriela Canziani, Raghuram Kalluri, Julie Lin, Michael P. Madaio

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Background: Anti-glomerular basement membrane (anti-GBM) antibody- mediated diseases are characterized by rapidly progressive glomerulonephritis (RPGN) that often results in irreversible loss of renal function and renal failure. Although many factors contribute to the fulminant nature and treatment resistance of this disease, we questioned whether high affinity autoantibody-α3(IV) collagen interactions lead to persistent antibody deposition, thereby perpetuating inflammation. To address this hypothesis, the binding kinetics of human anti-GBM antibodies (Ab) to α3(IV)NC1 were evaluated using an optical biosensor interaction analysis. Methods: Polyclonal anti-GBM Abs were purified by α3(IV)NC1 affinity chromatography from the sera of patients with anti-GBM AB-mediated diseases, including individuals with Goodpasture syndrome (GS), idiopathic RPGN (N = 7), and Alport syndrome (AL) following kidney transplantation (N = 4). The affinity- binding characteristics of the autoantibodies were determined using a biosensor analysis system, with immobilized bovine α3(IV)NC1 dimers. Results: All of the autoantibody preparations bound to α3(IV)NC1, whereas none bound to α1(IV)NC1 (control). Purified, normal serum IgG did not bind to either antigen. Estimated dissociation constants (K(d)) for the purified autoantibodies were 1.39E-04 - 7.30E-05 s-1 (GS) and 8.90E-05 2.80E-05 s-1 (AL). Their estimated association constants (K(a)) were 2.67E+04 ± 1.8E+04 (M-1s-1) and 2.76E+04 ± 1.70E+04 (M,1s-1) for GS and AL patients, respectively. By comparison with other Ab interactions, these Abs demonstrated high affinity, with relatively high on (binding) rates and slow off (dissociation) rates. Conclusions: The results suggest that anti-GBM Abs bind rapidly and remain tightly bound to the GBM in vivo. This property likely contributes to both the fulminant nature of this disease and its resistance to therapy, because persistent glomerular Ab deposition has the potential to produce continuous inflammation, despite removal of circulating Abs and adequate immunosuppression.

Original languageEnglish (US)
Pages (from-to)115-122
Number of pages8
JournalKidney International
Volume58
Issue number1
DOIs
StatePublished - Jan 1 2000

Fingerprint

Glomerular Basement Membrane
Anti-Glomerular Basement Membrane Disease
Autoantibodies
Collagen
Disease Resistance
Antibodies
Biosensing Techniques
Glomerulonephritis
Ephrin-A5
Hereditary Nephritis
Inflammation
Serum
Affinity Chromatography
Kidney Transplantation
Immunosuppression
Renal Insufficiency
Immunoglobulin G
Kidney
Antigens
Therapeutics

Keywords

  • Basement membrane
  • End-stage renal disease
  • Glomerulonephritis
  • Inflammation
  • Kidney transplantation
  • NCl domain
  • Optical biosensor

ASJC Scopus subject areas

  • Nephrology

Cite this

High affinity of anti-GBM antibodies from goodpasture and transplanted Alport patients to α3(IV)NC1 collagen. / Rutgers, Abraham; Meyers, Kevin E.C.; Canziani, Gabriela; Kalluri, Raghuram; Lin, Julie; Madaio, Michael P.

In: Kidney International, Vol. 58, No. 1, 01.01.2000, p. 115-122.

Research output: Contribution to journalArticle

Rutgers, Abraham ; Meyers, Kevin E.C. ; Canziani, Gabriela ; Kalluri, Raghuram ; Lin, Julie ; Madaio, Michael P. / High affinity of anti-GBM antibodies from goodpasture and transplanted Alport patients to α3(IV)NC1 collagen. In: Kidney International. 2000 ; Vol. 58, No. 1. pp. 115-122.
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AU - Rutgers, Abraham

AU - Meyers, Kevin E.C.

AU - Canziani, Gabriela

AU - Kalluri, Raghuram

AU - Lin, Julie

AU - Madaio, Michael P.

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Background: Anti-glomerular basement membrane (anti-GBM) antibody- mediated diseases are characterized by rapidly progressive glomerulonephritis (RPGN) that often results in irreversible loss of renal function and renal failure. Although many factors contribute to the fulminant nature and treatment resistance of this disease, we questioned whether high affinity autoantibody-α3(IV) collagen interactions lead to persistent antibody deposition, thereby perpetuating inflammation. To address this hypothesis, the binding kinetics of human anti-GBM antibodies (Ab) to α3(IV)NC1 were evaluated using an optical biosensor interaction analysis. Methods: Polyclonal anti-GBM Abs were purified by α3(IV)NC1 affinity chromatography from the sera of patients with anti-GBM AB-mediated diseases, including individuals with Goodpasture syndrome (GS), idiopathic RPGN (N = 7), and Alport syndrome (AL) following kidney transplantation (N = 4). The affinity- binding characteristics of the autoantibodies were determined using a biosensor analysis system, with immobilized bovine α3(IV)NC1 dimers. Results: All of the autoantibody preparations bound to α3(IV)NC1, whereas none bound to α1(IV)NC1 (control). Purified, normal serum IgG did not bind to either antigen. Estimated dissociation constants (K(d)) for the purified autoantibodies were 1.39E-04 - 7.30E-05 s-1 (GS) and 8.90E-05 2.80E-05 s-1 (AL). Their estimated association constants (K(a)) were 2.67E+04 ± 1.8E+04 (M-1s-1) and 2.76E+04 ± 1.70E+04 (M,1s-1) for GS and AL patients, respectively. By comparison with other Ab interactions, these Abs demonstrated high affinity, with relatively high on (binding) rates and slow off (dissociation) rates. Conclusions: The results suggest that anti-GBM Abs bind rapidly and remain tightly bound to the GBM in vivo. This property likely contributes to both the fulminant nature of this disease and its resistance to therapy, because persistent glomerular Ab deposition has the potential to produce continuous inflammation, despite removal of circulating Abs and adequate immunosuppression.

AB - Background: Anti-glomerular basement membrane (anti-GBM) antibody- mediated diseases are characterized by rapidly progressive glomerulonephritis (RPGN) that often results in irreversible loss of renal function and renal failure. Although many factors contribute to the fulminant nature and treatment resistance of this disease, we questioned whether high affinity autoantibody-α3(IV) collagen interactions lead to persistent antibody deposition, thereby perpetuating inflammation. To address this hypothesis, the binding kinetics of human anti-GBM antibodies (Ab) to α3(IV)NC1 were evaluated using an optical biosensor interaction analysis. Methods: Polyclonal anti-GBM Abs were purified by α3(IV)NC1 affinity chromatography from the sera of patients with anti-GBM AB-mediated diseases, including individuals with Goodpasture syndrome (GS), idiopathic RPGN (N = 7), and Alport syndrome (AL) following kidney transplantation (N = 4). The affinity- binding characteristics of the autoantibodies were determined using a biosensor analysis system, with immobilized bovine α3(IV)NC1 dimers. Results: All of the autoantibody preparations bound to α3(IV)NC1, whereas none bound to α1(IV)NC1 (control). Purified, normal serum IgG did not bind to either antigen. Estimated dissociation constants (K(d)) for the purified autoantibodies were 1.39E-04 - 7.30E-05 s-1 (GS) and 8.90E-05 2.80E-05 s-1 (AL). Their estimated association constants (K(a)) were 2.67E+04 ± 1.8E+04 (M-1s-1) and 2.76E+04 ± 1.70E+04 (M,1s-1) for GS and AL patients, respectively. By comparison with other Ab interactions, these Abs demonstrated high affinity, with relatively high on (binding) rates and slow off (dissociation) rates. Conclusions: The results suggest that anti-GBM Abs bind rapidly and remain tightly bound to the GBM in vivo. This property likely contributes to both the fulminant nature of this disease and its resistance to therapy, because persistent glomerular Ab deposition has the potential to produce continuous inflammation, despite removal of circulating Abs and adequate immunosuppression.

KW - Basement membrane

KW - End-stage renal disease

KW - Glomerulonephritis

KW - Inflammation

KW - Kidney transplantation

KW - NCl domain

KW - Optical biosensor

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