High-performance liquid chromatographic determination of amino acids and oligopeptides by pre-column fluorescence derivatization with 9-fluorenyl- methoxy carbonyl succinimide

Jinmao You, Xingjun Fan, Hongen Wang, Guojun Wang, Ji Xin Su, Changli Zhou

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

A sensitive high-performance chromatographic method for the detection of amino acids and oligopeptides with pre-column fluorescence derivatization has been developed. Glycine, glycylglycine, triglycine, glutathion, glutamic acid, and cysteine were separated on a reversed-phase C18 column with methanol-water-triethylamine as eluent; the derivatization and the chromatographic conditions were optimized. The six derivatives were eluted in 20 rain with good reproducibility. The relative standard deviations (n=6) at an analytical concentration of 2 x 10-6 M are less than 4.5%. The detection limits (signal-to-noise ratio=3) for the six derivatives are in the range of 15-46 fmol.

Original languageEnglish (US)
Pages (from-to)2103-2115
Number of pages13
JournalJournal of Liquid Chromatography and Related Technologies
Volume21
Issue number14
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

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