High performance liquid chromatographic determination of N- nitrosoamines by pre-column fluorescence derivatization with acridone-N- acetyl chloride

Jinmao You, Xingjun Fan, Wenjian Lao, Qingyu Ou, Qingcun Zhu

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A sensitive high performance liquid chromatographic method for the determination of N-nitrosoamines with pre-column fluorescence derivatization has been developed. N-nitrosoamines are first changed into secondary amines using denitrosation reagent, then react with acridone-N-acetyl chloride (ARC- Cl) to produce corresponding secondary amine derivatives, which exhibit a strong fluorescence. Maximum emission for ARC derivatives is 430 nm (λ(ex) 404 nm). The labelled derivatives are very stable, less than 4% decomposition occurs after heating at 40°C for 24 h. Fluorescence intensities of derivatives are higher in neutral and alkaline than in acidic solutions. This method, in conjunction with a multi-gradient program, offers a baseline resolution of the ARC derivatives from a linear acetonitrile gradient. Separation is carried out on a reverse phase C18 column. Derivatization and chromatographic conditions are optimized. The relative standard deviation (n = 6) at an analytical concentration of 10 pmol of each N-nitroamine is less than 4.5%. The detection limits at the fmol level. The method described is also suitable for analysis of other amino compounds in different biological samples.

Original languageEnglish (US)
Pages (from-to)437-449
Number of pages13
JournalTalanta
Volume48
Issue number2
DOIs
StatePublished - Jan 1 1999

Fingerprint

AIDS-Related Complex
Fluorescence
Derivatives
Amines
Liquids
Heating
Limit of Detection
acridone
acetyl chloride
Decomposition
acetonitrile

Keywords

  • Acridone-N-acetyl chloride (ARC-Cl)
  • Column liquid chromatography
  • Denitrosation
  • Fluorescence derivatization
  • N-nitroso compounds

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

High performance liquid chromatographic determination of N- nitrosoamines by pre-column fluorescence derivatization with acridone-N- acetyl chloride. / You, Jinmao; Fan, Xingjun; Lao, Wenjian; Ou, Qingyu; Zhu, Qingcun.

In: Talanta, Vol. 48, No. 2, 01.01.1999, p. 437-449.

Research output: Contribution to journalArticle

@article{a4ce95c1904e4215b37b1aaaef421746,
title = "High performance liquid chromatographic determination of N- nitrosoamines by pre-column fluorescence derivatization with acridone-N- acetyl chloride",
abstract = "A sensitive high performance liquid chromatographic method for the determination of N-nitrosoamines with pre-column fluorescence derivatization has been developed. N-nitrosoamines are first changed into secondary amines using denitrosation reagent, then react with acridone-N-acetyl chloride (ARC- Cl) to produce corresponding secondary amine derivatives, which exhibit a strong fluorescence. Maximum emission for ARC derivatives is 430 nm (λ(ex) 404 nm). The labelled derivatives are very stable, less than 4{\%} decomposition occurs after heating at 40°C for 24 h. Fluorescence intensities of derivatives are higher in neutral and alkaline than in acidic solutions. This method, in conjunction with a multi-gradient program, offers a baseline resolution of the ARC derivatives from a linear acetonitrile gradient. Separation is carried out on a reverse phase C18 column. Derivatization and chromatographic conditions are optimized. The relative standard deviation (n = 6) at an analytical concentration of 10 pmol of each N-nitroamine is less than 4.5{\%}. The detection limits at the fmol level. The method described is also suitable for analysis of other amino compounds in different biological samples.",
keywords = "Acridone-N-acetyl chloride (ARC-Cl), Column liquid chromatography, Denitrosation, Fluorescence derivatization, N-nitroso compounds",
author = "Jinmao You and Xingjun Fan and Wenjian Lao and Qingyu Ou and Qingcun Zhu",
year = "1999",
month = "1",
day = "1",
doi = "10.1016/S0039-9140(98)00265-3",
language = "English (US)",
volume = "48",
pages = "437--449",
journal = "Talanta",
issn = "0039-9140",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - High performance liquid chromatographic determination of N- nitrosoamines by pre-column fluorescence derivatization with acridone-N- acetyl chloride

AU - You, Jinmao

AU - Fan, Xingjun

AU - Lao, Wenjian

AU - Ou, Qingyu

AU - Zhu, Qingcun

PY - 1999/1/1

Y1 - 1999/1/1

N2 - A sensitive high performance liquid chromatographic method for the determination of N-nitrosoamines with pre-column fluorescence derivatization has been developed. N-nitrosoamines are first changed into secondary amines using denitrosation reagent, then react with acridone-N-acetyl chloride (ARC- Cl) to produce corresponding secondary amine derivatives, which exhibit a strong fluorescence. Maximum emission for ARC derivatives is 430 nm (λ(ex) 404 nm). The labelled derivatives are very stable, less than 4% decomposition occurs after heating at 40°C for 24 h. Fluorescence intensities of derivatives are higher in neutral and alkaline than in acidic solutions. This method, in conjunction with a multi-gradient program, offers a baseline resolution of the ARC derivatives from a linear acetonitrile gradient. Separation is carried out on a reverse phase C18 column. Derivatization and chromatographic conditions are optimized. The relative standard deviation (n = 6) at an analytical concentration of 10 pmol of each N-nitroamine is less than 4.5%. The detection limits at the fmol level. The method described is also suitable for analysis of other amino compounds in different biological samples.

AB - A sensitive high performance liquid chromatographic method for the determination of N-nitrosoamines with pre-column fluorescence derivatization has been developed. N-nitrosoamines are first changed into secondary amines using denitrosation reagent, then react with acridone-N-acetyl chloride (ARC- Cl) to produce corresponding secondary amine derivatives, which exhibit a strong fluorescence. Maximum emission for ARC derivatives is 430 nm (λ(ex) 404 nm). The labelled derivatives are very stable, less than 4% decomposition occurs after heating at 40°C for 24 h. Fluorescence intensities of derivatives are higher in neutral and alkaline than in acidic solutions. This method, in conjunction with a multi-gradient program, offers a baseline resolution of the ARC derivatives from a linear acetonitrile gradient. Separation is carried out on a reverse phase C18 column. Derivatization and chromatographic conditions are optimized. The relative standard deviation (n = 6) at an analytical concentration of 10 pmol of each N-nitroamine is less than 4.5%. The detection limits at the fmol level. The method described is also suitable for analysis of other amino compounds in different biological samples.

KW - Acridone-N-acetyl chloride (ARC-Cl)

KW - Column liquid chromatography

KW - Denitrosation

KW - Fluorescence derivatization

KW - N-nitroso compounds

UR - http://www.scopus.com/inward/record.url?scp=0032907220&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032907220&partnerID=8YFLogxK

U2 - 10.1016/S0039-9140(98)00265-3

DO - 10.1016/S0039-9140(98)00265-3

M3 - Article

VL - 48

SP - 437

EP - 449

JO - Talanta

JF - Talanta

SN - 0039-9140

IS - 2

ER -