TY - JOUR
T1 - High-performance liquid chromatography of sialooligosaccharides and gangliosides
AU - El Rassi, Ziad
AU - Horváth, Csaba
AU - Yu, Robert K.
AU - Ariga, Toshio
N1 - Funding Information:
This work was supported by Grant Nos. GM 20993, CA 21948 and NS 11853 from the National Institutes of Health, U.S. Department of Health and Human Services.
PY - 1989/3/17
Y1 - 1989/3/17
N2 - Glycans were cleaved from gangliosides and separated by high-performance liquid chromatography (HPLC). The columns were packed with bonded stationary phases made of microparticulate, macroporous silica with serotonin, phenylpropanolamine or tryptamine as the biogenic amine ligate. The ganglioside oligosaccharides were eluted in the order of increasing number of sialic acid residues in the molecule and their retention decreased with the ionic strength of the mobile phase. Best selectivity was obtained in the pH range from 3.0 to 4.0. The two major sialic acids, N-acetylneuraminic and N-glycolylneuraminic acids, were separated by lectin affinity chromatography using an HPLC column packed with silica-bound wheat germ agglutinin and 10 mM phosphate buffer, pH 4.0, as the eluent. Throughout this study, isocratic elution was used and the column effluent was monitored at 195 nm.
AB - Glycans were cleaved from gangliosides and separated by high-performance liquid chromatography (HPLC). The columns were packed with bonded stationary phases made of microparticulate, macroporous silica with serotonin, phenylpropanolamine or tryptamine as the biogenic amine ligate. The ganglioside oligosaccharides were eluted in the order of increasing number of sialic acid residues in the molecule and their retention decreased with the ionic strength of the mobile phase. Best selectivity was obtained in the pH range from 3.0 to 4.0. The two major sialic acids, N-acetylneuraminic and N-glycolylneuraminic acids, were separated by lectin affinity chromatography using an HPLC column packed with silica-bound wheat germ agglutinin and 10 mM phosphate buffer, pH 4.0, as the eluent. Throughout this study, isocratic elution was used and the column effluent was monitored at 195 nm.
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U2 - 10.1016/S0378-4347(00)82948-5
DO - 10.1016/S0378-4347(00)82948-5
M3 - Article
C2 - 2715282
AN - SCOPUS:0024972708
SN - 0378-4347
VL - 488
SP - 229
EP - 236
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - 1
ER -