TY - JOUR
T1 - High-performance thin-layer chromatography and densitometric determination of brain ganglioside compositions of several species
AU - Ando, Susumu
AU - Chang, Nan Chen
AU - Yu, Robert K.
N1 - Funding Information:
The technical assistance provided by Mrs. Nobuko Miyazawa is greatly appreciated. We also thank Mr. Frank Esposito for modifying the scanning mechanism of the densitometer. This work was supported by USPHS Grant NS 11853 and grants from the Kroc Foundation and the Esther A. and Joseph Klingenstein Fund.
PY - 1978/9
Y1 - 1978/9
N2 - Improved resolution of complex brain ganglioside mixtures was achieved by high-performance thin-layer chromatography. The percentage distribution of individual gangliosides was then determined by direct densitometric seanning, employing a transmittance mode, of the resorcinol-positive spots on the plate. As little as 90 pmol (29 ng) of lipid-bound sialic acid could be detected with a good signal-to-noise ratio. A linear detector response was observed up to 3.0 μg of lipid-bound sialic acid. The brain white matter ganglioside patterns of eight animal species, including human, chimpanzee, monkey, chicken, bovine, sheep, and pig, were examined in detail. In addition, human brain gray matter, rat cerebral, rat brain gray matter, and rat cerebellar ganglioside patterns were also studied. Ganglioside GM4 (G7) was found to be one of the major components in primate and chicken brain white matter, but it represented only a minor ganglioside in other species. Other major gangliosides in all brain samples studied were GM1, GD1a, GD1b, and GT1b. GM1 was more abundant in white matter than in gray matter. GT1a, a recently discovered ganglioside species, was found in all species examined, but was most abundant in the rat cerebellum. The latter source also contained high proportions of GT1b and GQ1b.
AB - Improved resolution of complex brain ganglioside mixtures was achieved by high-performance thin-layer chromatography. The percentage distribution of individual gangliosides was then determined by direct densitometric seanning, employing a transmittance mode, of the resorcinol-positive spots on the plate. As little as 90 pmol (29 ng) of lipid-bound sialic acid could be detected with a good signal-to-noise ratio. A linear detector response was observed up to 3.0 μg of lipid-bound sialic acid. The brain white matter ganglioside patterns of eight animal species, including human, chimpanzee, monkey, chicken, bovine, sheep, and pig, were examined in detail. In addition, human brain gray matter, rat cerebral, rat brain gray matter, and rat cerebellar ganglioside patterns were also studied. Ganglioside GM4 (G7) was found to be one of the major components in primate and chicken brain white matter, but it represented only a minor ganglioside in other species. Other major gangliosides in all brain samples studied were GM1, GD1a, GD1b, and GT1b. GM1 was more abundant in white matter than in gray matter. GT1a, a recently discovered ganglioside species, was found in all species examined, but was most abundant in the rat cerebellum. The latter source also contained high proportions of GT1b and GQ1b.
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U2 - 10.1016/0003-2697(78)90373-1
DO - 10.1016/0003-2697(78)90373-1
M3 - Article
C2 - 103458
AN - SCOPUS:0018080005
SN - 0003-2697
VL - 89
SP - 437
EP - 450
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -