Histochemical localization of gamma glutamyl transpeptidase in the paranodal region of peripheral nerve

B. J. Mrsulja, A. K. Gulati, A. A. Zalewski

Research output: Contribution to journalArticle

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Abstract

Gamma glutamyl transpeptidase (GGT), an amino acid transport enzyme, was investigated in normal and degenerated sciatic nerve of rat. The enzyme activity, which is considered to be a marker for cerebrovascular endothelium, was found to be absent in microvessels of normal and degenerated nerves. In the perineurium of normal nerve, GGT activity was faint, while in degenerated nerve, it increased. The most striking finding of this study was the observation of GGT activity at the paranode of each normal myelinated axon. It is interesting that after axotomy (8 weeks), no GGT activity was observed in the Schwann cells of degenerated nerve. Thus, Schwann cell plasmalemma contributed to GGT staining only when this cell was in contact with an axon mature enough to cause it to produce myelin. We conclude that, in peripheral nerve, transmembrane amino acid transport is apparently regional and associated with the paranodal region of myelinated nerve fibers.

Original languageEnglish (US)
Pages (from-to)1303-1308
Number of pages6
JournalJournal of Histochemistry and Cytochemistry
Volume32
Issue number12
DOIs
StatePublished - Jan 1 1984
Externally publishedYes

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gamma-Glutamyltransferase
Peripheral Nerves
Schwann Cells
Axons
Amino Acids
Axotomy
Myelinated Nerve Fibers
Sciatic Nerve
Enzymes
Myelin Sheath
Microvessels
Endothelium
Observation
Staining and Labeling

ASJC Scopus subject areas

  • Anatomy
  • Histology

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Histochemical localization of gamma glutamyl transpeptidase in the paranodal region of peripheral nerve. / Mrsulja, B. J.; Gulati, A. K.; Zalewski, A. A.

In: Journal of Histochemistry and Cytochemistry, Vol. 32, No. 12, 01.01.1984, p. 1303-1308.

Research output: Contribution to journalArticle

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