Histologic evaluation of osseous regeneration following combination therapy with platelet-rich plasma and bio-oss in a rat calvarial critical-size defect model

Philip J. Denicolo, M. Kelly Guyton, Michael F. Cuenin, Steven D. Hokett, Mohamed M.H. Sharawy, James Borke, James C. McPherson

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Platelet-rich plasma (PRP) is an autogenous source of growth factors shown to facilitate human bone growth. Bio-Oss, an osteoconductive xenograft, is used clinically to regenerate periodontal defects, restore dental alveolar ridges, and facilitate sinus-lift procedures. The purpose of this study was to analyze whether a combination of PRP and Bio-Oss would enhance bone regeneration better than either material alone. PRP and/or Bio-Oss were administered in an 8-mm critical-size defect (CSD) rat calvarial model of bone defect between 2 polytetrafluoroethylene membranes to prevent soft tissue incursion. Eight weeks after the induction of the CSD, histologic sections were stained with hematoxylin and eosin stain and analyzed via light microscopy. Qualitative analyses revealed new bone regeneration in all 4 groups. The Bio-Oss and PRP plus Bio-Oss groups demonstrated greater areas of closure in the defects than the control or PRP-only groups because of the space-maintaining ability of Bio-Oss. The groups grafted with Bio-Oss showed close contact with new bone growth throughout the defects, suggesting a stronger graft. The use of PRP alone or in combination with Bio-Oss, however, did not appear to enhance osseous regeneration at 8 weeks. Areas grafted with Bio-Oss demonstrated greater space-maintaining capacity than controls, and PRP was an effective vehicle for placement of the Bio-Oss. However, at 8 weeks this study was unable to demonstrate a significant advantage of using PRP plus Bio-Oss over using Bio-Oss alone.

Original languageEnglish (US)
Pages (from-to)543-549
Number of pages7
JournalJournal of Oral Implantology
Volume41
Issue number5
DOIs
StatePublished - Oct 1 2015

Fingerprint

Platelet-Rich Plasma
Regeneration
Therapeutics
Bone Regeneration
Bone Development
Bio-Oss
Alveolar Process
Polytetrafluoroethylene
Hematoxylin
Eosine Yellowish-(YS)
Heterografts
Microscopy
Intercellular Signaling Peptides and Proteins
Tooth
Coloring Agents

Keywords

  • Bio-oss
  • Bone regeneration
  • Platelet-rich plasma

ASJC Scopus subject areas

  • Oral Surgery

Cite this

Histologic evaluation of osseous regeneration following combination therapy with platelet-rich plasma and bio-oss in a rat calvarial critical-size defect model. / Denicolo, Philip J.; Guyton, M. Kelly; Cuenin, Michael F.; Hokett, Steven D.; Sharawy, Mohamed M.H.; Borke, James; McPherson, James C.

In: Journal of Oral Implantology, Vol. 41, No. 5, 01.10.2015, p. 543-549.

Research output: Contribution to journalArticle

Denicolo, Philip J. ; Guyton, M. Kelly ; Cuenin, Michael F. ; Hokett, Steven D. ; Sharawy, Mohamed M.H. ; Borke, James ; McPherson, James C. / Histologic evaluation of osseous regeneration following combination therapy with platelet-rich plasma and bio-oss in a rat calvarial critical-size defect model. In: Journal of Oral Implantology. 2015 ; Vol. 41, No. 5. pp. 543-549.
@article{7b626d4620b9423f8a4aa78fdc8d7f80,
title = "Histologic evaluation of osseous regeneration following combination therapy with platelet-rich plasma and bio-oss in a rat calvarial critical-size defect model",
abstract = "Platelet-rich plasma (PRP) is an autogenous source of growth factors shown to facilitate human bone growth. Bio-Oss, an osteoconductive xenograft, is used clinically to regenerate periodontal defects, restore dental alveolar ridges, and facilitate sinus-lift procedures. The purpose of this study was to analyze whether a combination of PRP and Bio-Oss would enhance bone regeneration better than either material alone. PRP and/or Bio-Oss were administered in an 8-mm critical-size defect (CSD) rat calvarial model of bone defect between 2 polytetrafluoroethylene membranes to prevent soft tissue incursion. Eight weeks after the induction of the CSD, histologic sections were stained with hematoxylin and eosin stain and analyzed via light microscopy. Qualitative analyses revealed new bone regeneration in all 4 groups. The Bio-Oss and PRP plus Bio-Oss groups demonstrated greater areas of closure in the defects than the control or PRP-only groups because of the space-maintaining ability of Bio-Oss. The groups grafted with Bio-Oss showed close contact with new bone growth throughout the defects, suggesting a stronger graft. The use of PRP alone or in combination with Bio-Oss, however, did not appear to enhance osseous regeneration at 8 weeks. Areas grafted with Bio-Oss demonstrated greater space-maintaining capacity than controls, and PRP was an effective vehicle for placement of the Bio-Oss. However, at 8 weeks this study was unable to demonstrate a significant advantage of using PRP plus Bio-Oss over using Bio-Oss alone.",
keywords = "Bio-oss, Bone regeneration, Platelet-rich plasma",
author = "Denicolo, {Philip J.} and Guyton, {M. Kelly} and Cuenin, {Michael F.} and Hokett, {Steven D.} and Sharawy, {Mohamed M.H.} and James Borke and McPherson, {James C.}",
year = "2015",
month = "10",
day = "1",
doi = "10.1563/AAID-JOI-D-12-00075",
language = "English (US)",
volume = "41",
pages = "543--549",
journal = "Journal of Oral Implantology",
issn = "0160-6972",
publisher = "Allen Press Inc.",
number = "5",

}

TY - JOUR

T1 - Histologic evaluation of osseous regeneration following combination therapy with platelet-rich plasma and bio-oss in a rat calvarial critical-size defect model

AU - Denicolo, Philip J.

AU - Guyton, M. Kelly

AU - Cuenin, Michael F.

AU - Hokett, Steven D.

AU - Sharawy, Mohamed M.H.

AU - Borke, James

AU - McPherson, James C.

PY - 2015/10/1

Y1 - 2015/10/1

N2 - Platelet-rich plasma (PRP) is an autogenous source of growth factors shown to facilitate human bone growth. Bio-Oss, an osteoconductive xenograft, is used clinically to regenerate periodontal defects, restore dental alveolar ridges, and facilitate sinus-lift procedures. The purpose of this study was to analyze whether a combination of PRP and Bio-Oss would enhance bone regeneration better than either material alone. PRP and/or Bio-Oss were administered in an 8-mm critical-size defect (CSD) rat calvarial model of bone defect between 2 polytetrafluoroethylene membranes to prevent soft tissue incursion. Eight weeks after the induction of the CSD, histologic sections were stained with hematoxylin and eosin stain and analyzed via light microscopy. Qualitative analyses revealed new bone regeneration in all 4 groups. The Bio-Oss and PRP plus Bio-Oss groups demonstrated greater areas of closure in the defects than the control or PRP-only groups because of the space-maintaining ability of Bio-Oss. The groups grafted with Bio-Oss showed close contact with new bone growth throughout the defects, suggesting a stronger graft. The use of PRP alone or in combination with Bio-Oss, however, did not appear to enhance osseous regeneration at 8 weeks. Areas grafted with Bio-Oss demonstrated greater space-maintaining capacity than controls, and PRP was an effective vehicle for placement of the Bio-Oss. However, at 8 weeks this study was unable to demonstrate a significant advantage of using PRP plus Bio-Oss over using Bio-Oss alone.

AB - Platelet-rich plasma (PRP) is an autogenous source of growth factors shown to facilitate human bone growth. Bio-Oss, an osteoconductive xenograft, is used clinically to regenerate periodontal defects, restore dental alveolar ridges, and facilitate sinus-lift procedures. The purpose of this study was to analyze whether a combination of PRP and Bio-Oss would enhance bone regeneration better than either material alone. PRP and/or Bio-Oss were administered in an 8-mm critical-size defect (CSD) rat calvarial model of bone defect between 2 polytetrafluoroethylene membranes to prevent soft tissue incursion. Eight weeks after the induction of the CSD, histologic sections were stained with hematoxylin and eosin stain and analyzed via light microscopy. Qualitative analyses revealed new bone regeneration in all 4 groups. The Bio-Oss and PRP plus Bio-Oss groups demonstrated greater areas of closure in the defects than the control or PRP-only groups because of the space-maintaining ability of Bio-Oss. The groups grafted with Bio-Oss showed close contact with new bone growth throughout the defects, suggesting a stronger graft. The use of PRP alone or in combination with Bio-Oss, however, did not appear to enhance osseous regeneration at 8 weeks. Areas grafted with Bio-Oss demonstrated greater space-maintaining capacity than controls, and PRP was an effective vehicle for placement of the Bio-Oss. However, at 8 weeks this study was unable to demonstrate a significant advantage of using PRP plus Bio-Oss over using Bio-Oss alone.

KW - Bio-oss

KW - Bone regeneration

KW - Platelet-rich plasma

UR - http://www.scopus.com/inward/record.url?scp=84945188867&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84945188867&partnerID=8YFLogxK

U2 - 10.1563/AAID-JOI-D-12-00075

DO - 10.1563/AAID-JOI-D-12-00075

M3 - Article

VL - 41

SP - 543

EP - 549

JO - Journal of Oral Implantology

JF - Journal of Oral Implantology

SN - 0160-6972

IS - 5

ER -