Histone deacetylase inhibition destabilizes the multi-potent state of uncommitted adipose-derived mesenchymal stromal cells

Amel Dudakovic, Emily T. Camilleri, Eric A. Lewallen, Meghan Elizabeth McGee Lawrence, Scott M. Riester, Sanjeev Kakar, Martin Montecino, Gary S. Stein, Hyun Mo Ryoo, Allan B. Dietz, Jennifer J. Westendorf, Andre J. Van Wijnen

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Human adipose-derived mesenchymal stromal cells (AMSCs) grown in platelet lysate are promising agents for therapeutic tissue regeneration. Here, we investigated whether manipulation of epigenetic events by the clinically relevant histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA) alters differentiation of AMSCs. The multipotency of AMSCs was validated by their ability to differentiate into osteogenic, chondrogenic, and adipogenic lineages. High-throughput RNA sequencing and RT-qPCR established that human histone deacetylases (HDAC1 to HDAC11, and SIRT1 to SIRT7) are differentially expressed in AMSCs. SAHA induces hyperacetylation of histone H3 and H4, stimulates protein expression of the HDAC-responsive gene SLC9A3R1/NHERF1 and modulates the AKT/FOXO1 pathway. Biologically, SAHA interferes with osteogenic, chondrogenic and adipogenic lineage commitment of multipotent AMSCs. Mechanistically, SAHA-induced loss of differentiation potential of uncommitted AMSCs correlates with multiple changes in the expression of principal transcription factors that control mesenchymal or pluripotent states. We propose that SAHA destabilizes the multi-potent epigenetic state of uncommitted human AMSCs by hyper-acetylation and perturbation of key transcription factor pathways. Furthermore, AMSCs grown in platelet lysate may provide a useful biological model for screening of new HDAC inhibitors that control the biological fate of human mesenchymal stromal cells.

Original languageEnglish (US)
Pages (from-to)52-62
Number of pages11
JournalJournal of Cellular Physiology
Volume230
Issue number1
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Fingerprint

Histone Deacetylases
Mesenchymal Stromal Cells
Cells
Histone Deacetylase Inhibitors
Platelets
Histones
Transcription Factors
Acetylation
Tissue regeneration
Epigenomics
Blood Platelets
Screening
Genes
Throughput
vorinostat
High-Throughput Nucleotide Sequencing
RNA
Biological Models
Regeneration
Proteins

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Histone deacetylase inhibition destabilizes the multi-potent state of uncommitted adipose-derived mesenchymal stromal cells. / Dudakovic, Amel; Camilleri, Emily T.; Lewallen, Eric A.; McGee Lawrence, Meghan Elizabeth; Riester, Scott M.; Kakar, Sanjeev; Montecino, Martin; Stein, Gary S.; Ryoo, Hyun Mo; Dietz, Allan B.; Westendorf, Jennifer J.; Van Wijnen, Andre J.

In: Journal of Cellular Physiology, Vol. 230, No. 1, 01.01.2015, p. 52-62.

Research output: Contribution to journalArticle

Dudakovic, A, Camilleri, ET, Lewallen, EA, McGee Lawrence, ME, Riester, SM, Kakar, S, Montecino, M, Stein, GS, Ryoo, HM, Dietz, AB, Westendorf, JJ & Van Wijnen, AJ 2015, 'Histone deacetylase inhibition destabilizes the multi-potent state of uncommitted adipose-derived mesenchymal stromal cells', Journal of Cellular Physiology, vol. 230, no. 1, pp. 52-62. https://doi.org/10.1002/jcp.24680
Dudakovic, Amel ; Camilleri, Emily T. ; Lewallen, Eric A. ; McGee Lawrence, Meghan Elizabeth ; Riester, Scott M. ; Kakar, Sanjeev ; Montecino, Martin ; Stein, Gary S. ; Ryoo, Hyun Mo ; Dietz, Allan B. ; Westendorf, Jennifer J. ; Van Wijnen, Andre J. / Histone deacetylase inhibition destabilizes the multi-potent state of uncommitted adipose-derived mesenchymal stromal cells. In: Journal of Cellular Physiology. 2015 ; Vol. 230, No. 1. pp. 52-62.
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