Histone deacetylase inhibition promotes osteoblast maturation by altering the histone H4 epigenome and reduces akt phosphorylation

Amel Dudakovic, Jared M. Evans, Ying Li, Sumit Middha, Meghan Elizabeth McGee Lawrence, Andre J. Van Wijnen, Jennifer J. Westendorf

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Background:Histone deacetylase (HDAC) inhibition promotes bone formationin vitrovia undefined epigenetic events. Results:Microarray and ChIP-Seq analyses revealed genomic areas where H4 acetylation is altered by HDAC inhibitors and identified differentially regulated genes. Conclusion:Suberoylanilide hydroxamic acid increases H4 acetylation and suppresses phosphorylation of insulin/Akt signaling mediators in osteoblasts. Significance:Epigenetic profiling is a powerful means to gain mechanistic insights into bone anabolic processes.

Original languageEnglish (US)
Pages (from-to)28783-28791
Number of pages9
JournalJournal of Biological Chemistry
Volume288
Issue number40
DOIs
StatePublished - Oct 4 2013
Externally publishedYes

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Acetylation
Phosphorylation
Histone Deacetylases
Osteoblasts
Epigenomics
Histones
Bone
Bone and Bones
Histone Deacetylase Inhibitors
Microarrays
Genes
Insulin
vorinostat

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Histone deacetylase inhibition promotes osteoblast maturation by altering the histone H4 epigenome and reduces akt phosphorylation. / Dudakovic, Amel; Evans, Jared M.; Li, Ying; Middha, Sumit; McGee Lawrence, Meghan Elizabeth; Van Wijnen, Andre J.; Westendorf, Jennifer J.

In: Journal of Biological Chemistry, Vol. 288, No. 40, 04.10.2013, p. 28783-28791.

Research output: Contribution to journalArticle

Dudakovic, Amel ; Evans, Jared M. ; Li, Ying ; Middha, Sumit ; McGee Lawrence, Meghan Elizabeth ; Van Wijnen, Andre J. ; Westendorf, Jennifer J. / Histone deacetylase inhibition promotes osteoblast maturation by altering the histone H4 epigenome and reduces akt phosphorylation. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 40. pp. 28783-28791.
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