HLA-DQβ57 in Hispanic patients with insulin-dependent diabetes mellitus

Grace Lee, Fayek N. Shamma, Michael Peter Diamond, John T.D. Lee

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: The purpose of our study was to investigate the distribution of HLA-DQ β-chain amino acid residue 57 (HLA-DQβ57) as a genetic marker of susceptibility for insulin-dependent diabetes mellitus in the Hispanic population. STUDY DESIGN: Fifteen patients of Puerto Rican descent with juvenile-onset insulin-dependent diabetes mellitus underwent human leukocyte antigen typing for HLA-DOβ57 by polymerase chain reaction amplification of the target genomic DQ sequence followed by hybridization of the polymerase chain reaction product to phosphorus 32-labeled allele-specific oligonucleotide probes. A control. group of 44 Hispanic adults without diabetes who were undergoing human leukocyte antigen typing for tissue donation were concurrently typed for comparison. RESULTS: The Hispanic insulin-dependent diabetes mellitus group showed a significant increase in homozygosity for a non-aspartate amino acid (p = 0.023) over a control group of Hispanic subjects without diabetes. A high rate of heterozygosity for aspartate (53.3%) is found in Hispanic subjects with insulin-dependent diabetes mellitus as well. CONCLUSIONS: HLA-DQβ57 in the Hispanic population has a distribution distinct from HLA-DQβ57 in the Caucasian population. A single aspartate is not protective against insulin-dependent diabetes mellitus in Hispanic subjects.

Original languageEnglish (US)
Pages (from-to)1565-1570
Number of pages6
JournalAmerican Journal of Obstetrics and Gynecology
Volume167
Issue number6
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

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Type 1 Diabetes Mellitus
Hispanic Americans
HLA Antigens
Aspartic Acid
Population
HLA-DQ Antigens
Amino Acids
Histocompatibility Testing
Polymerase Chain Reaction
Tissue and Organ Procurement
Oligonucleotide Probes
Genetic Predisposition to Disease
Genetic Markers
Phosphorus
Alleles
Control Groups

Keywords

  • HLA-DQβ57
  • Hispanic insulin-dependent diabetes mellitus
  • Major histocompatibility complex
  • autoimmune diabetes

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

HLA-DQβ57 in Hispanic patients with insulin-dependent diabetes mellitus. / Lee, Grace; Shamma, Fayek N.; Diamond, Michael Peter; Lee, John T.D.

In: American Journal of Obstetrics and Gynecology, Vol. 167, No. 6, 01.01.1992, p. 1565-1570.

Research output: Contribution to journalArticle

Lee, Grace ; Shamma, Fayek N. ; Diamond, Michael Peter ; Lee, John T.D. / HLA-DQβ57 in Hispanic patients with insulin-dependent diabetes mellitus. In: American Journal of Obstetrics and Gynecology. 1992 ; Vol. 167, No. 6. pp. 1565-1570.
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