HPLC of amino acids and oligopeptides by pre-column fluorescence derivatization with 9-acridine formyl chloride

Jin Mao You, Xin Jun Fan, Qing Yu Ou

Research output: Contribution to journalArticle

Abstract

A highly sensitive HPLC method for the detection of amino acids and oligopeptides with 9-acridine formyl chloride by pre-column fluorescence derivatization has been developed. Glycine, glycylglycine, histidine, triglycine and glutathione were separated on a reversed-phase C 18 column with methanol-water-triethylamine eluent, derivatization and chromatographic conditions were optimized. The five derivatives were eluted in 28 min with a good reproducibility. Linear range of the calibration graph was 0.08-260 nmol/ml -1. The relative standard deviations(n=6) are < 5%. Detection limits (signal-to-noise ratio=3) for the five derivatives are 20-40 fmol.

Original languageEnglish (US)
Pages (from-to)875-878
Number of pages4
JournalChinese Chemical Letters
Volume8
Issue number10
Publication statusPublished - Dec 1 1997
Externally publishedYes

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ASJC Scopus subject areas

  • Chemistry(all)

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