HPLC of amino acids and oligopeptides by pre-column fluorescence derivatization with 9-acridine formyl chloride

Jin Mao You, Xingjun Fan, Qing Yu Ou

Research output: Contribution to journalArticle

Abstract

A highly sensitive HPLC method for the detection of amino acids and oligopeptides with 9-acridine formyl chloride by pre-column fluorescence derivatization has been developed. Glycine, glycylglycine, histidine, triglycine and glutathione were separated on a reversed-phase C 18 column with methanol-water-triethylamine eluent, derivatization and chromatographic conditions were optimized. The five derivatives were eluted in 28 min with a good reproducibility. Linear range of the calibration graph was 0.08-260 nmol/ml -1. The relative standard deviations(n=6) are < 5%. Detection limits (signal-to-noise ratio=3) for the five derivatives are 20-40 fmol.

Original languageEnglish (US)
Pages (from-to)875-878
Number of pages4
JournalChinese Chemical Letters
Volume8
Issue number10
StatePublished - Dec 1 1997

Fingerprint

Acridines
Oligopeptides
glycyl-glycyl-glycine
Chlorides
Fluorescence
Glycylglycine
Derivatives
Amino Acids
Histidine
Glycine
Glutathione
Methanol
Signal to noise ratio
Calibration
Water
triethylamine

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

HPLC of amino acids and oligopeptides by pre-column fluorescence derivatization with 9-acridine formyl chloride. / You, Jin Mao; Fan, Xingjun; Ou, Qing Yu.

In: Chinese Chemical Letters, Vol. 8, No. 10, 01.12.1997, p. 875-878.

Research output: Contribution to journalArticle

@article{97451b516fcf478ea38765621c3427fa,
title = "HPLC of amino acids and oligopeptides by pre-column fluorescence derivatization with 9-acridine formyl chloride",
abstract = "A highly sensitive HPLC method for the detection of amino acids and oligopeptides with 9-acridine formyl chloride by pre-column fluorescence derivatization has been developed. Glycine, glycylglycine, histidine, triglycine and glutathione were separated on a reversed-phase C 18 column with methanol-water-triethylamine eluent, derivatization and chromatographic conditions were optimized. The five derivatives were eluted in 28 min with a good reproducibility. Linear range of the calibration graph was 0.08-260 nmol/ml -1. The relative standard deviations(n=6) are < 5{\%}. Detection limits (signal-to-noise ratio=3) for the five derivatives are 20-40 fmol.",
author = "You, {Jin Mao} and Xingjun Fan and Ou, {Qing Yu}",
year = "1997",
month = "12",
day = "1",
language = "English (US)",
volume = "8",
pages = "875--878",
journal = "Chinese Chemical Letters",
issn = "1001-8417",
publisher = "Elsevier",
number = "10",

}

TY - JOUR

T1 - HPLC of amino acids and oligopeptides by pre-column fluorescence derivatization with 9-acridine formyl chloride

AU - You, Jin Mao

AU - Fan, Xingjun

AU - Ou, Qing Yu

PY - 1997/12/1

Y1 - 1997/12/1

N2 - A highly sensitive HPLC method for the detection of amino acids and oligopeptides with 9-acridine formyl chloride by pre-column fluorescence derivatization has been developed. Glycine, glycylglycine, histidine, triglycine and glutathione were separated on a reversed-phase C 18 column with methanol-water-triethylamine eluent, derivatization and chromatographic conditions were optimized. The five derivatives were eluted in 28 min with a good reproducibility. Linear range of the calibration graph was 0.08-260 nmol/ml -1. The relative standard deviations(n=6) are < 5%. Detection limits (signal-to-noise ratio=3) for the five derivatives are 20-40 fmol.

AB - A highly sensitive HPLC method for the detection of amino acids and oligopeptides with 9-acridine formyl chloride by pre-column fluorescence derivatization has been developed. Glycine, glycylglycine, histidine, triglycine and glutathione were separated on a reversed-phase C 18 column with methanol-water-triethylamine eluent, derivatization and chromatographic conditions were optimized. The five derivatives were eluted in 28 min with a good reproducibility. Linear range of the calibration graph was 0.08-260 nmol/ml -1. The relative standard deviations(n=6) are < 5%. Detection limits (signal-to-noise ratio=3) for the five derivatives are 20-40 fmol.

UR - http://www.scopus.com/inward/record.url?scp=0031503921&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031503921&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0031503921

VL - 8

SP - 875

EP - 878

JO - Chinese Chemical Letters

JF - Chinese Chemical Letters

SN - 1001-8417

IS - 10

ER -