HSP40 binding is the first step in the HSP90 chaperoning pathway for the progesterone receptor

M. Patricia Hernández, Ahmed Chadli, David O. Toft

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

The progesterone receptor (PR) can be isolated in its native conformation able to bind hormone, yet its ligand-binding domain rapidly loses its activity at elevated temperature. However, an in vitro chaperoning system consisting of five proteins (HSP40, HSP70, HOP, HSP90, and p23) with ATP is capable of restoring this function. The first step of this chaperoning mechanism is usually thought to be the binding of HSP70 to PR. Our findings here show that the binding of HSP40 to PR is, instead, the first step. HSP40 binding occurred rapidly and was not dependent on ATP or other proteins. The stoichiometry of HSP40 to native PR in these complexes was ∼1:1. HSP40 bound specifically and with a high affinity to native PR (Kd = 77 nM). The binding of HSP40 to PR was sustained and did not interact in the highly dynamic fashion that has been observed previously for HSP90 in this system. The HSP40·PR complex could be isolated as a functional unit that could, after the addition of the other chaperones, progress to a PR complex capable of hormone binding. These results indicate that HSP40 initiates the entry of PR into the HSP90 pathway.

Original languageEnglish (US)
Pages (from-to)11873-11881
Number of pages9
JournalJournal of Biological Chemistry
Volume277
Issue number14
DOIs
StatePublished - Apr 5 2002

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Progesterone Receptors
HSP40 Heat-Shock Proteins
Adenosine Triphosphate
Hormones
Stoichiometry
Conformations
Ligands
Temperature

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

HSP40 binding is the first step in the HSP90 chaperoning pathway for the progesterone receptor. / Patricia Hernández, M.; Chadli, Ahmed; Toft, David O.

In: Journal of Biological Chemistry, Vol. 277, No. 14, 05.04.2002, p. 11873-11881.

Research output: Contribution to journalArticle

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