Human α fetoprotein enhances epidermal growth factor proliferative activity upon porcine granulosa cells in monolayer culture

Juan A. Leal, Jeffrey V. May, Brooks Allen Keel

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

α fetoprotein (AFP) is present at high concentrations in fetal fluids, certain neoplasias, and regenerating liver. Its physiological function remains largely unknown. Using a primary monolayer culture system, we investigated the proliferative activity of human (h) cord blood (CB) and highly purified AFP. hAFP, purified from hCB by Cibacron blue and immunoaffinity chromatography was homogeneous on SDS-PAGE and silver stain. Porcine granulosa cells from ovarian small follicles were cultured (25, 000/cm2) for 2 days in medium (Ham’s F-12: DMEM, 1:1) + 5% fetal calf serum (FCS) to facilitate attachment, followed by 6 days in medium containing: FCS, hCB or h amniotic fluid (1-20%)+/- EGF (10 ng/ml); or 0.25% plasma-derived serum (PDS) containing human low density lipoprotein (LDL, 25 ug/ml), +/- AFP (0.05-5 ug), and +/- EGF and IGF-I (10 ng/ml). In this system, single growth factors do not stimulate proliferation, a characteristic also exhibited by AFP. When combined with EGF, however, AFP dose-dependently increased proliferation to levels equal to that obtained with 10% FCS (2.3-fold increase vs PDS/LDL controls). When combined with EGF+IGF-I, AFP again dose-dependently increased proliferation to levels equal to that obtained with 10% FCS+EGF.

Original languageEnglish (US)
Pages (from-to)669-671
Number of pages3
JournalEndocrinology
Volume126
Issue number1
DOIs
StatePublished - Jan 1 1990

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Fetal Proteins
Granulosa Cells
Epidermal Growth Factor
Swine
Serum
Insulin-Like Growth Factor I
Ovarian Follicle
Amniotic Fluid
Fetal Blood
LDL Lipoproteins
Silver
Human Activities
Chromatography
Polyacrylamide Gel Electrophoresis
Intercellular Signaling Peptides and Proteins
Coloring Agents
Liver
Neoplasms

ASJC Scopus subject areas

  • Endocrinology

Cite this

Human α fetoprotein enhances epidermal growth factor proliferative activity upon porcine granulosa cells in monolayer culture. / Leal, Juan A.; May, Jeffrey V.; Keel, Brooks Allen.

In: Endocrinology, Vol. 126, No. 1, 01.01.1990, p. 669-671.

Research output: Contribution to journalArticle

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abstract = "α fetoprotein (AFP) is present at high concentrations in fetal fluids, certain neoplasias, and regenerating liver. Its physiological function remains largely unknown. Using a primary monolayer culture system, we investigated the proliferative activity of human (h) cord blood (CB) and highly purified AFP. hAFP, purified from hCB by Cibacron blue and immunoaffinity chromatography was homogeneous on SDS-PAGE and silver stain. Porcine granulosa cells from ovarian small follicles were cultured (25, 000/cm2) for 2 days in medium (Ham’s F-12: DMEM, 1:1) + 5{\%} fetal calf serum (FCS) to facilitate attachment, followed by 6 days in medium containing: FCS, hCB or h amniotic fluid (1-20{\%})+/- EGF (10 ng/ml); or 0.25{\%} plasma-derived serum (PDS) containing human low density lipoprotein (LDL, 25 ug/ml), +/- AFP (0.05-5 ug), and +/- EGF and IGF-I (10 ng/ml). In this system, single growth factors do not stimulate proliferation, a characteristic also exhibited by AFP. When combined with EGF, however, AFP dose-dependently increased proliferation to levels equal to that obtained with 10{\%} FCS (2.3-fold increase vs PDS/LDL controls). When combined with EGF+IGF-I, AFP again dose-dependently increased proliferation to levels equal to that obtained with 10{\%} FCS+EGF.",
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