Human α‐fetoprotein purified from amniotic fluid enhances growth factor‐mediated cell proliferation in vitro

Brooks Allen Keel, Kevan B. Eddy, Sechin Cho, Jeffrey V. May

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Using a primary monolayer culture of porcine granulosa cells (pGC) as an in vitro cell proliferation assay, we have examined the growth‐promoting activity of α‐fetoprotein (AFP) purified from term cord blood and midtrimester amniotic fluid. Increasing concentrations (2.5–20%) of crude human cord blood (CB) increased pGC proliferation, while identical concentrations of crude amniotic fluid (AF) were ineffective. When the cell system was maximally stimulated, AF dose dependently decreased cell proliferation. AFP purified from AF and CB (1.25–5.0 μg/ml) was not mitogenic alone, but, in the presence of epidermal growth factor (EGF) + insulin‐like growth factor (IGF‐I) (10 ng/ml each), AFP dose dependently increased cell proliferation to nearly double that of EGF + IGF‐I alone. The response of pGC to the proliferative effects of AF‐AFP and CB‐AFP were identical at each dose of AFP tested. These results indicate that although crude, pooled midtrimester AF does not display the mitogenic activity seen in cord blood, AFP purified from pooled AF significantly synergizes with growth factors to increase cell proliferation markedly.

Original languageEnglish (US)
Pages (from-to)112-118
Number of pages7
JournalMolecular Reproduction and Development
Volume30
Issue number2
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

Fingerprint

Fetal Proteins
Amniotic Fluid
Cell Proliferation
Fetal Blood
Granulosa Cells
Growth
Swine
Second Pregnancy Trimester
Epidermal Growth Factor
Intercellular Signaling Peptides and Proteins
In Vitro Techniques

Keywords

  • AFP
  • Cord blood
  • Porcine granulosa cells

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Human α‐fetoprotein purified from amniotic fluid enhances growth factor‐mediated cell proliferation in vitro. / Keel, Brooks Allen; Eddy, Kevan B.; Cho, Sechin; May, Jeffrey V.

In: Molecular Reproduction and Development, Vol. 30, No. 2, 01.01.1991, p. 112-118.

Research output: Contribution to journalArticle

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