TY - JOUR
T1 - Human angiogenic cell precursors restore function in the infarcted rat heart
T2 - A comparison of cell delivery routes
AU - Sun, Zhuo
AU - Wu, Jun
AU - Fujii, Hiroko
AU - Wu, Jiang
AU - Li, Shu Hong
AU - Porozov, Svetlana
AU - Belleli, Adina
AU - Fulga, Valentin
AU - Porat, Yael
AU - Li, Ren Ke
PY - 2008/6
Y1 - 2008/6
N2 - Background: We recently isolated angiogenic cell precursors (ACPs) from human blood, which can induce angiogenesis in vitro. Aims: In the present study, we used a nude rat model of ischaemic cardiomyopathy to compare the efficacy of intramyocardial and intracoronary ACP implantation, and to evaluate effects on cardiac function, scar size and angiogenesis. Methods and results: Adult nude rats underwent coronary artery ligation. Six days later, ACPs (characterized in vitro prior to implantation) or culture media were injected directly into the ischaemic myocardial region or into the coronary artery via the aorta. Cardiac function was measured by echocardiography prior to and at 2 and 4 weeks after implantation. Scar morphology, cell engraftment, and myocardial angiogenesis were evaluated at 4 weeks. Two and four weeks after implantation, cardiac function declined in both of the control groups but improved in both the intramyocardial and intracoronary ACP groups. Significant reductions in myocardial scar area were only observed in the intramyocardial ACP group, while increases in blood vessel density, which were observed in all ACP recipients, were greatest in the intracoronary ACP group. Conclusions: Human ACPs, delivered via intramyocardial or intracoronary injection, engrafted into damaged cardiac tissue and improved cardiac function within 4 weeks through effects on scar morphology and blood vessel formation.
AB - Background: We recently isolated angiogenic cell precursors (ACPs) from human blood, which can induce angiogenesis in vitro. Aims: In the present study, we used a nude rat model of ischaemic cardiomyopathy to compare the efficacy of intramyocardial and intracoronary ACP implantation, and to evaluate effects on cardiac function, scar size and angiogenesis. Methods and results: Adult nude rats underwent coronary artery ligation. Six days later, ACPs (characterized in vitro prior to implantation) or culture media were injected directly into the ischaemic myocardial region or into the coronary artery via the aorta. Cardiac function was measured by echocardiography prior to and at 2 and 4 weeks after implantation. Scar morphology, cell engraftment, and myocardial angiogenesis were evaluated at 4 weeks. Two and four weeks after implantation, cardiac function declined in both of the control groups but improved in both the intramyocardial and intracoronary ACP groups. Significant reductions in myocardial scar area were only observed in the intramyocardial ACP group, while increases in blood vessel density, which were observed in all ACP recipients, were greatest in the intracoronary ACP group. Conclusions: Human ACPs, delivered via intramyocardial or intracoronary injection, engrafted into damaged cardiac tissue and improved cardiac function within 4 weeks through effects on scar morphology and blood vessel formation.
KW - Angiogenesis
KW - Angiogenic cell precursors
KW - Cell transplantation
KW - Heart failure
KW - Myocardial ischaemia
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U2 - 10.1016/j.ejheart.2008.04.004
DO - 10.1016/j.ejheart.2008.04.004
M3 - Article
C2 - 18490195
AN - SCOPUS:44649190397
SN - 1388-9842
VL - 10
SP - 525
EP - 533
JO - European Journal of Heart Failure
JF - European Journal of Heart Failure
IS - 6
ER -