Human corneal storage in modified McCarey-Kaufman and K-Sol media: Effect on endothelial Na+/K+ ATPase pump site density and permeability

M. L. McDermott, M. A. Watsky, D. H. Geroski, H. F. Edelhauser

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Endothelial permeability (Pac) to carboxyfluorescein and Na+/K+ ATPase pump site density were determined in human corneas following storage for 4 or 7 days at 4°C in either modified McCarey-Kaufman (mMK) or K-Sol media. Following 4 days of storage, Pac values for mMK-and K-Sol-preserved corneas were not significantly different from those of their prestorage mates. After 7 days of storage, however, corneas stored in K-Sol media showed a significant increase in Pac compared to their prestorage mates, whereas the mMK-stored corneas showed no change in Pac. Na+/K+ ATPase pump site density determined using [3H]ouabain was similar to a control group in the K-Sol-stored tissue but higher in the mMK-stored tissue following 7 days of storage. These studies suggest that mMK medium maintains endothelial barrier function and Na+/K+ ATPase pump site density at least as well as K-Sol medium through 7 days of corneal storage.

Original languageEnglish (US)
Pages (from-to)44-49
Number of pages6
JournalCornea
Volume10
Issue number1
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology

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