TY - JOUR
T1 - Human dendritic cells respond to Porphyromonas gingivalis LPS by promoting a Th2 effector response in vitro
AU - Jotwani, Ravi
AU - Pulendran, Bali
AU - Agrawal, Sudhanshu
AU - Cutler, Christopher W.
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2003/11
Y1 - 2003/11
N2 - Understanding how mucosal pathogens modulate the immune response may facilitate the development of vaccines for disparate human diseases. In the present study, human monocyte-derived DC (MDDC) were pulsed with LPS of the oral pathogen Porphyromonas gingivalis and Escherichia coli 25922 and analyzed for: (i) production of Th-biasing/inflammatory cytokines; (ii) maturation/costimulatory molecules; and (iii) induction of allogeneic CD4+ and naive CD45RA+ T cell proliferation and release of Th1 or Th2 cytokines. We show that E. coli LPS-pulsed MDDC released Th1-biasing cytokines - consisting of high levels of IL-12 p70, IFN-γ-inducible protein 10 (IP-10) - but also TNF-α, IL-10, IL-6 ana IL-1β. In contrast, no IL-12 p70 or IP-10, and lower levels of TNF-α and IL-10 were induced by P. gingivalis LPS. These differences were sustained at LPS doses that yielded nearly equivalent maturation of MDDC; moreover the T cell response was consistent: E. coli LPS-pulsed MDDC induced higher T cell proliferation, and T cells released more IFN-γ and IL-2, but less IL-5 than T cells co-cultured with P. gingivalis LPS pulsed-MDDC. IL-13 was secreted by naive CD45RA+CD45RO-CD4+T cells in response to P. gingivalisLPS-pulsed MDDC. These results suggest that human MDDC can be polarized by LPS from the mucosal pathogen P. gingivalis to induce a Th2 effector response in vitro.
AB - Understanding how mucosal pathogens modulate the immune response may facilitate the development of vaccines for disparate human diseases. In the present study, human monocyte-derived DC (MDDC) were pulsed with LPS of the oral pathogen Porphyromonas gingivalis and Escherichia coli 25922 and analyzed for: (i) production of Th-biasing/inflammatory cytokines; (ii) maturation/costimulatory molecules; and (iii) induction of allogeneic CD4+ and naive CD45RA+ T cell proliferation and release of Th1 or Th2 cytokines. We show that E. coli LPS-pulsed MDDC released Th1-biasing cytokines - consisting of high levels of IL-12 p70, IFN-γ-inducible protein 10 (IP-10) - but also TNF-α, IL-10, IL-6 ana IL-1β. In contrast, no IL-12 p70 or IP-10, and lower levels of TNF-α and IL-10 were induced by P. gingivalis LPS. These differences were sustained at LPS doses that yielded nearly equivalent maturation of MDDC; moreover the T cell response was consistent: E. coli LPS-pulsed MDDC induced higher T cell proliferation, and T cells released more IFN-γ and IL-2, but less IL-5 than T cells co-cultured with P. gingivalis LPS pulsed-MDDC. IL-13 was secreted by naive CD45RA+CD45RO-CD4+T cells in response to P. gingivalisLPS-pulsed MDDC. These results suggest that human MDDC can be polarized by LPS from the mucosal pathogen P. gingivalis to induce a Th2 effector response in vitro.
KW - Cytokine
KW - Human
KW - Lipopolysaccharide
KW - Monocyte-derived dendritic cell
KW - Porphyromonas gingivalis
UR - http://www.scopus.com/inward/record.url?scp=0242391998&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0242391998&partnerID=8YFLogxK
U2 - 10.1002/eji.200324392
DO - 10.1002/eji.200324392
M3 - Article
C2 - 14579266
AN - SCOPUS:0242391998
VL - 33
SP - 2980
EP - 2986
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 11
ER -