Abstract
Understanding how mucosal pathogens modulate the immune response may facilitate the development of vaccines for disparate human diseases. In the present study, human monocyte-derived DC (MDDC) were pulsed with LPS of the oral pathogen Porphyromonas gingivalis and Escherichia coli 25922 and analyzed for: (i) production of Th-biasing/inflammatory cytokines; (ii) maturation/costimulatory molecules; and (iii) induction of allogeneic CD4+ and naive CD45RA+ T cell proliferation and release of Th1 or Th2 cytokines. We show that E. coli LPS-pulsed MDDC released Th1-biasing cytokines - consisting of high levels of IL-12 p70, IFN-γ-inducible protein 10 (IP-10) - but also TNF-α, IL-10, IL-6 ana IL-1β. In contrast, no IL-12 p70 or IP-10, and lower levels of TNF-α and IL-10 were induced by P. gingivalis LPS. These differences were sustained at LPS doses that yielded nearly equivalent maturation of MDDC; moreover the T cell response was consistent: E. coli LPS-pulsed MDDC induced higher T cell proliferation, and T cells released more IFN-γ and IL-2, but less IL-5 than T cells co-cultured with P. gingivalis LPS pulsed-MDDC. IL-13 was secreted by naive CD45RA+CD45RO-CD4+T cells in response to P. gingivalisLPS-pulsed MDDC. These results suggest that human MDDC can be polarized by LPS from the mucosal pathogen P. gingivalis to induce a Th2 effector response in vitro.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2980-2986 |
| Number of pages | 7 |
| Journal | European Journal of Immunology |
| Volume | 33 |
| Issue number | 11 |
| DOIs | |
| State | Published - Nov 1 2003 |
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Keywords
- Cytokine
- Human
- Lipopolysaccharide
- Monocyte-derived dendritic cell
- Porphyromonas gingivalis
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
Human dendritic cells respond to Porphyromonas gingivalis LPS by promoting a Th2 effector response in vitro. / Jotwani, Ravi; Pulendran, Bali; Agrawal, Sudhanshu; Cutler, Christopher W.
In: European Journal of Immunology, Vol. 33, No. 11, 01.11.2003, p. 2980-2986.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Human dendritic cells respond to Porphyromonas gingivalis LPS by promoting a Th2 effector response in vitro
AU - Jotwani, Ravi
AU - Pulendran, Bali
AU - Agrawal, Sudhanshu
AU - Cutler, Christopher W
PY - 2003/11/1
Y1 - 2003/11/1
N2 - Understanding how mucosal pathogens modulate the immune response may facilitate the development of vaccines for disparate human diseases. In the present study, human monocyte-derived DC (MDDC) were pulsed with LPS of the oral pathogen Porphyromonas gingivalis and Escherichia coli 25922 and analyzed for: (i) production of Th-biasing/inflammatory cytokines; (ii) maturation/costimulatory molecules; and (iii) induction of allogeneic CD4+ and naive CD45RA+ T cell proliferation and release of Th1 or Th2 cytokines. We show that E. coli LPS-pulsed MDDC released Th1-biasing cytokines - consisting of high levels of IL-12 p70, IFN-γ-inducible protein 10 (IP-10) - but also TNF-α, IL-10, IL-6 ana IL-1β. In contrast, no IL-12 p70 or IP-10, and lower levels of TNF-α and IL-10 were induced by P. gingivalis LPS. These differences were sustained at LPS doses that yielded nearly equivalent maturation of MDDC; moreover the T cell response was consistent: E. coli LPS-pulsed MDDC induced higher T cell proliferation, and T cells released more IFN-γ and IL-2, but less IL-5 than T cells co-cultured with P. gingivalis LPS pulsed-MDDC. IL-13 was secreted by naive CD45RA+CD45RO-CD4+T cells in response to P. gingivalisLPS-pulsed MDDC. These results suggest that human MDDC can be polarized by LPS from the mucosal pathogen P. gingivalis to induce a Th2 effector response in vitro.
AB - Understanding how mucosal pathogens modulate the immune response may facilitate the development of vaccines for disparate human diseases. In the present study, human monocyte-derived DC (MDDC) were pulsed with LPS of the oral pathogen Porphyromonas gingivalis and Escherichia coli 25922 and analyzed for: (i) production of Th-biasing/inflammatory cytokines; (ii) maturation/costimulatory molecules; and (iii) induction of allogeneic CD4+ and naive CD45RA+ T cell proliferation and release of Th1 or Th2 cytokines. We show that E. coli LPS-pulsed MDDC released Th1-biasing cytokines - consisting of high levels of IL-12 p70, IFN-γ-inducible protein 10 (IP-10) - but also TNF-α, IL-10, IL-6 ana IL-1β. In contrast, no IL-12 p70 or IP-10, and lower levels of TNF-α and IL-10 were induced by P. gingivalis LPS. These differences were sustained at LPS doses that yielded nearly equivalent maturation of MDDC; moreover the T cell response was consistent: E. coli LPS-pulsed MDDC induced higher T cell proliferation, and T cells released more IFN-γ and IL-2, but less IL-5 than T cells co-cultured with P. gingivalis LPS pulsed-MDDC. IL-13 was secreted by naive CD45RA+CD45RO-CD4+T cells in response to P. gingivalisLPS-pulsed MDDC. These results suggest that human MDDC can be polarized by LPS from the mucosal pathogen P. gingivalis to induce a Th2 effector response in vitro.
KW - Cytokine
KW - Human
KW - Lipopolysaccharide
KW - Monocyte-derived dendritic cell
KW - Porphyromonas gingivalis
UR - http://www.scopus.com/inward/record.url?scp=0242391998&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0242391998&partnerID=8YFLogxK
U2 - 10.1002/eji.200324392
DO - 10.1002/eji.200324392
M3 - Article
C2 - 14579266
AN - SCOPUS:0242391998
VL - 33
SP - 2980
EP - 2986
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 11
ER -