Human dendritic cells respond to Porphyromonas gingivalis LPS by promoting a Th2 effector response in vitro

Ravi Jotwani, Bali Pulendran, Sudhanshu Agrawal, Christopher W Cutler

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Understanding how mucosal pathogens modulate the immune response may facilitate the development of vaccines for disparate human diseases. In the present study, human monocyte-derived DC (MDDC) were pulsed with LPS of the oral pathogen Porphyromonas gingivalis and Escherichia coli 25922 and analyzed for: (i) production of Th-biasing/inflammatory cytokines; (ii) maturation/costimulatory molecules; and (iii) induction of allogeneic CD4+ and naive CD45RA+ T cell proliferation and release of Th1 or Th2 cytokines. We show that E. coli LPS-pulsed MDDC released Th1-biasing cytokines - consisting of high levels of IL-12 p70, IFN-γ-inducible protein 10 (IP-10) - but also TNF-α, IL-10, IL-6 ana IL-1β. In contrast, no IL-12 p70 or IP-10, and lower levels of TNF-α and IL-10 were induced by P. gingivalis LPS. These differences were sustained at LPS doses that yielded nearly equivalent maturation of MDDC; moreover the T cell response was consistent: E. coli LPS-pulsed MDDC induced higher T cell proliferation, and T cells released more IFN-γ and IL-2, but less IL-5 than T cells co-cultured with P. gingivalis LPS pulsed-MDDC. IL-13 was secreted by naive CD45RA+CD45RO-CD4+T cells in response to P. gingivalisLPS-pulsed MDDC. These results suggest that human MDDC can be polarized by LPS from the mucosal pathogen P. gingivalis to induce a Th2 effector response in vitro.

Original languageEnglish (US)
Pages (from-to)2980-2986
Number of pages7
JournalEuropean Journal of Immunology
Volume33
Issue number11
DOIs
StatePublished - Nov 1 2003

Fingerprint

Porphyromonas gingivalis
Dendritic Cells
Monocytes
T-Lymphocytes
Interleukin-12
Cytokines
Escherichia coli
Interleukin-10
Cell Proliferation
Interleukin-13
Interleukin-5
In Vitro Techniques
Interleukin-1
Interleukin-2
Interleukin-6
Proteins
Vaccines

Keywords

  • Cytokine
  • Human
  • Lipopolysaccharide
  • Monocyte-derived dendritic cell
  • Porphyromonas gingivalis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Human dendritic cells respond to Porphyromonas gingivalis LPS by promoting a Th2 effector response in vitro. / Jotwani, Ravi; Pulendran, Bali; Agrawal, Sudhanshu; Cutler, Christopher W.

In: European Journal of Immunology, Vol. 33, No. 11, 01.11.2003, p. 2980-2986.

Research output: Contribution to journalArticle

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