Abstract
The complete gene for human fatty acid ethyl ester Synthase-III (FAEES-III) was isolated from a human genomic lambda phage library for functional and structural determination. The gene spans approximately 3.3 kb which includes 791 base pairs of the 5' and 124 base pairs of the 3' flanking regions. The gene is comprised of seven exons and is interrupted by six introns. Several transcription regulatory sequences were identified in the promoter region. Primer extension experiments demonstrated the existence of two possible transcription initiation sites at nucleotide -29 and 32 position, 5' to the start of the translation. In addition to a TATA box at position -29 relative to the transcription initiation site and two Spl GGGCGG recognition sequences at nucleotide positions -42 to -37 and -50 to -45, the promoter contains a sequence motif matching the transcription activating factor AP-1. We also found an A + T rich region between nucleotide -505 and -390 which contained twenty-two AAAAT tandem repeats. The gene for FAEES-III was localized to human chromosome 11 by hybridizing the genomic fragment Xh01 to Chinese hamster/human somatic cell hybrid panels. These data extend our knowledge of non-oxidative alcohol metabolism and permit linkage analyses between this pathway and alcohol-related phenotypes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 145-151 |
| Number of pages | 7 |
| Journal | Molecular and Cellular Biochemistry |
| Volume | 173 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Sep 25 1997 |
| Externally published | Yes |
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Keywords
- Chromosome
- End organ
- Linkage
- Phenotypes
- Toxic
- Transcription
ASJC Scopus subject areas
- Molecular Biology
- Clinical Biochemistry
- Cell Biology
Cite this
Human fatty acid ethyl ester synthase-III gene : Genomic organization, nucleotide sequencing and chromosomal localization. / Bora, Puran S.; Guruge, Bandula L.; Douglas Miller, D.; Chairman, Bernard R.; Fortson, Wilbert.
In: Molecular and Cellular Biochemistry, Vol. 173, No. 1-2, 25.09.1997, p. 145-151.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Human fatty acid ethyl ester synthase-III gene
T2 - Genomic organization, nucleotide sequencing and chromosomal localization
AU - Bora, Puran S.
AU - Guruge, Bandula L.
AU - Douglas Miller, D.
AU - Chairman, Bernard R.
AU - Fortson, Wilbert
PY - 1997/9/25
Y1 - 1997/9/25
N2 - The complete gene for human fatty acid ethyl ester Synthase-III (FAEES-III) was isolated from a human genomic lambda phage library for functional and structural determination. The gene spans approximately 3.3 kb which includes 791 base pairs of the 5' and 124 base pairs of the 3' flanking regions. The gene is comprised of seven exons and is interrupted by six introns. Several transcription regulatory sequences were identified in the promoter region. Primer extension experiments demonstrated the existence of two possible transcription initiation sites at nucleotide -29 and 32 position, 5' to the start of the translation. In addition to a TATA box at position -29 relative to the transcription initiation site and two Spl GGGCGG recognition sequences at nucleotide positions -42 to -37 and -50 to -45, the promoter contains a sequence motif matching the transcription activating factor AP-1. We also found an A + T rich region between nucleotide -505 and -390 which contained twenty-two AAAAT tandem repeats. The gene for FAEES-III was localized to human chromosome 11 by hybridizing the genomic fragment Xh01 to Chinese hamster/human somatic cell hybrid panels. These data extend our knowledge of non-oxidative alcohol metabolism and permit linkage analyses between this pathway and alcohol-related phenotypes.
AB - The complete gene for human fatty acid ethyl ester Synthase-III (FAEES-III) was isolated from a human genomic lambda phage library for functional and structural determination. The gene spans approximately 3.3 kb which includes 791 base pairs of the 5' and 124 base pairs of the 3' flanking regions. The gene is comprised of seven exons and is interrupted by six introns. Several transcription regulatory sequences were identified in the promoter region. Primer extension experiments demonstrated the existence of two possible transcription initiation sites at nucleotide -29 and 32 position, 5' to the start of the translation. In addition to a TATA box at position -29 relative to the transcription initiation site and two Spl GGGCGG recognition sequences at nucleotide positions -42 to -37 and -50 to -45, the promoter contains a sequence motif matching the transcription activating factor AP-1. We also found an A + T rich region between nucleotide -505 and -390 which contained twenty-two AAAAT tandem repeats. The gene for FAEES-III was localized to human chromosome 11 by hybridizing the genomic fragment Xh01 to Chinese hamster/human somatic cell hybrid panels. These data extend our knowledge of non-oxidative alcohol metabolism and permit linkage analyses between this pathway and alcohol-related phenotypes.
KW - Chromosome
KW - End organ
KW - Linkage
KW - Phenotypes
KW - Toxic
KW - Transcription
UR - http://www.scopus.com/inward/record.url?scp=0030861440&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030861440&partnerID=8YFLogxK
U2 - 10.1023/A:1006892030277
DO - 10.1023/A:1006892030277
M3 - Article
C2 - 9278265
AN - SCOPUS:0030861440
VL - 173
SP - 145
EP - 151
JO - Molecular and Cellular Biochemistry
JF - Molecular and Cellular Biochemistry
SN - 0300-8177
IS - 1-2
ER -