Human fatty acid ethyl ester synthase-III gene: Genomic organization, nucleotide sequencing and chromosomal localization

Puran S. Bora, Bandula L. Guruge, Donald D Miller, Bernard R. Chairman, Wilbert Fortson

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The complete gene for human fatty acid ethyl ester Synthase-III (FAEES-III) was isolated from a human genomic lambda phage library for functional and structural determination. The gene spans approximately 3.3 kb which includes 791 base pairs of the 5' and 124 base pairs of the 3' flanking regions. The gene is comprised of seven exons and is interrupted by six introns. Several transcription regulatory sequences were identified in the promoter region. Primer extension experiments demonstrated the existence of two possible transcription initiation sites at nucleotide -29 and 32 position, 5' to the start of the translation. In addition to a TATA box at position -29 relative to the transcription initiation site and two Spl GGGCGG recognition sequences at nucleotide positions -42 to -37 and -50 to -45, the promoter contains a sequence motif matching the transcription activating factor AP-1. We also found an A + T rich region between nucleotide -505 and -390 which contained twenty-two AAAAT tandem repeats. The gene for FAEES-III was localized to human chromosome 11 by hybridizing the genomic fragment Xh01 to Chinese hamster/human somatic cell hybrid panels. These data extend our knowledge of non-oxidative alcohol metabolism and permit linkage analyses between this pathway and alcohol-related phenotypes.

Original languageEnglish (US)
Pages (from-to)145-151
Number of pages7
JournalMolecular and Cellular Biochemistry
Volume173
Issue number1-2
DOIs
StatePublished - Sep 25 1997
Externally publishedYes

Fingerprint

Nucleotides
Genes
Transcription Initiation Site
Base Pairing
Activating Transcription Factors
Alcohols
3' Flanking Region
Bacteriophage lambda
Chromosomes, Human, Pair 11
TATA Box
Bacteriophages
Tandem Repeat Sequences
Hybrid Cells
Transcription Factor AP-1
Human Chromosomes
Transcription
Chromosomes
Cricetulus
Genetic Promoter Regions
Metabolism

Keywords

  • Chromosome
  • End organ
  • Linkage
  • Phenotypes
  • Toxic
  • Transcription

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Human fatty acid ethyl ester synthase-III gene : Genomic organization, nucleotide sequencing and chromosomal localization. / Bora, Puran S.; Guruge, Bandula L.; Miller, Donald D; Chairman, Bernard R.; Fortson, Wilbert.

In: Molecular and Cellular Biochemistry, Vol. 173, No. 1-2, 25.09.1997, p. 145-151.

Research output: Contribution to journalArticle

Bora, Puran S. ; Guruge, Bandula L. ; Miller, Donald D ; Chairman, Bernard R. ; Fortson, Wilbert. / Human fatty acid ethyl ester synthase-III gene : Genomic organization, nucleotide sequencing and chromosomal localization. In: Molecular and Cellular Biochemistry. 1997 ; Vol. 173, No. 1-2. pp. 145-151.
@article{2ee4ced2c04a4f9f8d1e75d31e6afe89,
title = "Human fatty acid ethyl ester synthase-III gene: Genomic organization, nucleotide sequencing and chromosomal localization",
abstract = "The complete gene for human fatty acid ethyl ester Synthase-III (FAEES-III) was isolated from a human genomic lambda phage library for functional and structural determination. The gene spans approximately 3.3 kb which includes 791 base pairs of the 5' and 124 base pairs of the 3' flanking regions. The gene is comprised of seven exons and is interrupted by six introns. Several transcription regulatory sequences were identified in the promoter region. Primer extension experiments demonstrated the existence of two possible transcription initiation sites at nucleotide -29 and 32 position, 5' to the start of the translation. In addition to a TATA box at position -29 relative to the transcription initiation site and two Spl GGGCGG recognition sequences at nucleotide positions -42 to -37 and -50 to -45, the promoter contains a sequence motif matching the transcription activating factor AP-1. We also found an A + T rich region between nucleotide -505 and -390 which contained twenty-two AAAAT tandem repeats. The gene for FAEES-III was localized to human chromosome 11 by hybridizing the genomic fragment Xh01 to Chinese hamster/human somatic cell hybrid panels. These data extend our knowledge of non-oxidative alcohol metabolism and permit linkage analyses between this pathway and alcohol-related phenotypes.",
keywords = "Chromosome, End organ, Linkage, Phenotypes, Toxic, Transcription",
author = "Bora, {Puran S.} and Guruge, {Bandula L.} and Miller, {Donald D} and Chairman, {Bernard R.} and Wilbert Fortson",
year = "1997",
month = "9",
day = "25",
doi = "10.1023/A:1006892030277",
language = "English (US)",
volume = "173",
pages = "145--151",
journal = "Molecular and Cellular Biochemistry",
issn = "0300-8177",
publisher = "Springer Netherlands",
number = "1-2",

}

TY - JOUR

T1 - Human fatty acid ethyl ester synthase-III gene

T2 - Genomic organization, nucleotide sequencing and chromosomal localization

AU - Bora, Puran S.

AU - Guruge, Bandula L.

AU - Miller, Donald D

AU - Chairman, Bernard R.

AU - Fortson, Wilbert

PY - 1997/9/25

Y1 - 1997/9/25

N2 - The complete gene for human fatty acid ethyl ester Synthase-III (FAEES-III) was isolated from a human genomic lambda phage library for functional and structural determination. The gene spans approximately 3.3 kb which includes 791 base pairs of the 5' and 124 base pairs of the 3' flanking regions. The gene is comprised of seven exons and is interrupted by six introns. Several transcription regulatory sequences were identified in the promoter region. Primer extension experiments demonstrated the existence of two possible transcription initiation sites at nucleotide -29 and 32 position, 5' to the start of the translation. In addition to a TATA box at position -29 relative to the transcription initiation site and two Spl GGGCGG recognition sequences at nucleotide positions -42 to -37 and -50 to -45, the promoter contains a sequence motif matching the transcription activating factor AP-1. We also found an A + T rich region between nucleotide -505 and -390 which contained twenty-two AAAAT tandem repeats. The gene for FAEES-III was localized to human chromosome 11 by hybridizing the genomic fragment Xh01 to Chinese hamster/human somatic cell hybrid panels. These data extend our knowledge of non-oxidative alcohol metabolism and permit linkage analyses between this pathway and alcohol-related phenotypes.

AB - The complete gene for human fatty acid ethyl ester Synthase-III (FAEES-III) was isolated from a human genomic lambda phage library for functional and structural determination. The gene spans approximately 3.3 kb which includes 791 base pairs of the 5' and 124 base pairs of the 3' flanking regions. The gene is comprised of seven exons and is interrupted by six introns. Several transcription regulatory sequences were identified in the promoter region. Primer extension experiments demonstrated the existence of two possible transcription initiation sites at nucleotide -29 and 32 position, 5' to the start of the translation. In addition to a TATA box at position -29 relative to the transcription initiation site and two Spl GGGCGG recognition sequences at nucleotide positions -42 to -37 and -50 to -45, the promoter contains a sequence motif matching the transcription activating factor AP-1. We also found an A + T rich region between nucleotide -505 and -390 which contained twenty-two AAAAT tandem repeats. The gene for FAEES-III was localized to human chromosome 11 by hybridizing the genomic fragment Xh01 to Chinese hamster/human somatic cell hybrid panels. These data extend our knowledge of non-oxidative alcohol metabolism and permit linkage analyses between this pathway and alcohol-related phenotypes.

KW - Chromosome

KW - End organ

KW - Linkage

KW - Phenotypes

KW - Toxic

KW - Transcription

UR - http://www.scopus.com/inward/record.url?scp=0030861440&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030861440&partnerID=8YFLogxK

U2 - 10.1023/A:1006892030277

DO - 10.1023/A:1006892030277

M3 - Article

C2 - 9278265

AN - SCOPUS:0030861440

VL - 173

SP - 145

EP - 151

JO - Molecular and Cellular Biochemistry

JF - Molecular and Cellular Biochemistry

SN - 0300-8177

IS - 1-2

ER -