Human interstitial retinol-binding protein (IRBP): cloning, partial sequence, and chromosomal localization

Gregory I Liou, S. L. Fong, J. Gosden, P. van Tuinen, D. H. Ledbetter, S. Christie, D. Rout, S. Bhattacharya, R. G. Cook, Y. Li, C. Wang, C. D.B. Bridges

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Abstract

A cloned 2184-bp cDNA coding for human interstitial retinol-binding protein (IRBP) has been isolated and sequenced. The probe hybridized to a 5.2-kb poly(A) RNA from human retinas. Nineteen tryptic peptides (363 amino acids) sequenced and purified from bovine IRBP could be aligned with 86-88% homology to the translated sequence. Two segments approximately 200 amino acids long were found to have a 41% residue identity, suggesting an internal duplication event. This cloned cDNA was used to probe DNA samples from a panel of 29 rodent-human somatic cell hybrids, mapping the structural gene for IRBP to chromosome 10. In situ hybridization suggested a regional localization near the centromere (p11.2→q11.2), although a secondary site of hybridization at q24→25 was also observed.

Original languageEnglish (US)
Pages (from-to)315-323
Number of pages9
JournalSomatic Cell and Molecular Genetics
Volume13
Issue number4
DOIs
Publication statusPublished - Jul 1 1987

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ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Liou, G. I., Fong, S. L., Gosden, J., van Tuinen, P., Ledbetter, D. H., Christie, S., ... Bridges, C. D. B. (1987). Human interstitial retinol-binding protein (IRBP): cloning, partial sequence, and chromosomal localization. Somatic Cell and Molecular Genetics, 13(4), 315-323. https://doi.org/10.1007/BF01534925