Human interstitial retinol-binding protein (IRBP): cloning, partial sequence, and chromosomal localization

Gregory I Liou, S. L. Fong, J. Gosden, P. van Tuinen, D. H. Ledbetter, S. Christie, D. Rout, S. Bhattacharya, R. G. Cook, Y. Li, C. Wang, C. D.B. Bridges

Research output: Contribution to journalArticle

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Abstract

A cloned 2184-bp cDNA coding for human interstitial retinol-binding protein (IRBP) has been isolated and sequenced. The probe hybridized to a 5.2-kb poly(A) RNA from human retinas. Nineteen tryptic peptides (363 amino acids) sequenced and purified from bovine IRBP could be aligned with 86-88% homology to the translated sequence. Two segments approximately 200 amino acids long were found to have a 41% residue identity, suggesting an internal duplication event. This cloned cDNA was used to probe DNA samples from a panel of 29 rodent-human somatic cell hybrids, mapping the structural gene for IRBP to chromosome 10. In situ hybridization suggested a regional localization near the centromere (p11.2→q11.2), although a secondary site of hybridization at q24→25 was also observed.

Original languageEnglish (US)
Pages (from-to)315-323
Number of pages9
JournalSomatic Cell and Molecular Genetics
Volume13
Issue number4
DOIs
StatePublished - Jul 1 1987

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Organism Cloning
Complementary DNA
Amino Acids
Chromosomes, Human, Pair 10
Centromere
Hybrid Cells
Chromosome Mapping
DNA Probes
In Situ Hybridization
Retina
Rodentia
Messenger RNA
Peptides
interstitial retinol-binding protein

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Liou, G. I., Fong, S. L., Gosden, J., van Tuinen, P., Ledbetter, D. H., Christie, S., ... Bridges, C. D. B. (1987). Human interstitial retinol-binding protein (IRBP): cloning, partial sequence, and chromosomal localization. Somatic Cell and Molecular Genetics, 13(4), 315-323. https://doi.org/10.1007/BF01534925

Human interstitial retinol-binding protein (IRBP) : cloning, partial sequence, and chromosomal localization. / Liou, Gregory I; Fong, S. L.; Gosden, J.; van Tuinen, P.; Ledbetter, D. H.; Christie, S.; Rout, D.; Bhattacharya, S.; Cook, R. G.; Li, Y.; Wang, C.; Bridges, C. D.B.

In: Somatic Cell and Molecular Genetics, Vol. 13, No. 4, 01.07.1987, p. 315-323.

Research output: Contribution to journalArticle

Liou, GI, Fong, SL, Gosden, J, van Tuinen, P, Ledbetter, DH, Christie, S, Rout, D, Bhattacharya, S, Cook, RG, Li, Y, Wang, C & Bridges, CDB 1987, 'Human interstitial retinol-binding protein (IRBP): cloning, partial sequence, and chromosomal localization', Somatic Cell and Molecular Genetics, vol. 13, no. 4, pp. 315-323. https://doi.org/10.1007/BF01534925
Liou, Gregory I ; Fong, S. L. ; Gosden, J. ; van Tuinen, P. ; Ledbetter, D. H. ; Christie, S. ; Rout, D. ; Bhattacharya, S. ; Cook, R. G. ; Li, Y. ; Wang, C. ; Bridges, C. D.B. / Human interstitial retinol-binding protein (IRBP) : cloning, partial sequence, and chromosomal localization. In: Somatic Cell and Molecular Genetics. 1987 ; Vol. 13, No. 4. pp. 315-323.
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