Human mammary tumor cell proliferation: primary role of platelet-derived growth factor and possible synergism with human α-fetoprotein

Juan A. Leal, Bhushan K. Gangrade, John L. Kiser, Jeffrey V. May, Brooks Allen Keel

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Human mammary medullary carcinoma cells (passages 16 to 21) were cultured for 2 days to allow for attachment, followed by 6 days of culture in either fetal calf serum, human cord blood, human amniotic fluid, or growth factors in the presence or absence of purified human α-fetoprotein (AFP). When growth factors were tested alone, only platelet-derived growth factor produced a significant increase in cell proliferation. Although up to 40% amniotic fluid had no effect on cell proliferation, human cord blood was two-fold more potent than fetal calf serum at similar concentrations. The addition of 10 ng/ml of platelet-derived growth factor increased the proliferative activity of human cord blood 1.5- to 2.5-fold. Ablation of endogenous AFP by affinity chromatography reduced the proliferative activity of cord blood by 75%. Similarly, the mitogenic activity of cord blood plus platelet-derived growth factor was reduced by 56% when AFP was removed. Purified AFP dose-dependently enhanced the proliferative activity of platelet-derived growth factor. This synergistic effect was specific for platelet-derived growth factor. We conclude that platelet-derived growth factor is a major growth factor controlling the proliferation of these tumor cells and that AFP may enhance growth factor proliferative activity and human mammary tumor growth.

Original languageEnglish (US)
Pages (from-to)247-251
Number of pages5
JournalSteroids
Volume56
Issue number5
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

Fingerprint

Fetal Proteins
Platelet-Derived Growth Factor
Cell proliferation
Tumors
Fetal Blood
Cell Proliferation
Breast Neoplasms
Blood
Intercellular Signaling Peptides and Proteins
Amniotic Fluid
Human Activities
Cells
Affinity chromatography
Medullary Carcinoma
Fluids
Ablation
Serum
Affinity Chromatography
Blood Platelets
Growth

Keywords

  • (AFP)
  • cancer cells
  • cell proliferation
  • mammary tumor cells
  • mammary tumor growth in vitro
  • mitogenic activity of AFP
  • oncofetal proteins
  • platelet-derived growth factor
  • α-fetoprotein

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology
  • Pharmacology
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Human mammary tumor cell proliferation : primary role of platelet-derived growth factor and possible synergism with human α-fetoprotein. / Leal, Juan A.; Gangrade, Bhushan K.; Kiser, John L.; May, Jeffrey V.; Keel, Brooks Allen.

In: Steroids, Vol. 56, No. 5, 01.01.1991, p. 247-251.

Research output: Contribution to journalArticle

Leal, Juan A. ; Gangrade, Bhushan K. ; Kiser, John L. ; May, Jeffrey V. ; Keel, Brooks Allen. / Human mammary tumor cell proliferation : primary role of platelet-derived growth factor and possible synergism with human α-fetoprotein. In: Steroids. 1991 ; Vol. 56, No. 5. pp. 247-251.
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