@article{bb4d9fc8646c4d9289ec14ce40e36786,
title = "Human papillomavirus genotypes in women with invasive cervical cancer with and without human immunodeficiency virus infection in Botswana",
abstract = "Cervical cancer remains a significant cause of morbidity and mortality in women worldwide and is the leading cause of cancer-related death in Botswana. It is well established that women with HIV have a higher risk of persistent HPV infection leading to cervical cancer. We assessed HPV prevalence and genotype distribution in 126 tissue specimens from confirmed invasive cervical cancer cases using Abbott real-time PCR assay. Overall, 88 (69.8%) women were HIV-infected. Fifty-seven (64.8%) of the HIV-infected women had a baseline CD4+ count ≥350 cells/μl, and 82 (93.2%) were on antiretroviral therapy at the time of cervical cancer diagnosis. The median age of HIV-infected patients was significantly younger than that of HIV-uninfected patients (p < 0.001). HPV DNA was detected in all of 126 (100%) of tissues analyzed in our study. The HPV genotypes identified included the HPV-16 (75.4%), HPV-18 (28.6%) and other high-risk (hr) HPV genotypes (16.7%). HIV infection was positively associated with the presence of the HPV-16 genotype (p = 0.036), but not with HPV-18 or with other high-risk (hr)-HPV genotypes. Thirty-three percent of the patients had multiple hr-HPV genotypes, with higher rates in HIV-infected women. These results highlight the importance and potential impact of large-scale HPV vaccination programs covering HPV-16 and HPV-18 genotypes in countries like Botswana with high burden of HIV infection.",
keywords = "Botswana, HIV, HPV-16, HPV-18, invasive cervical cancer, multiple infections",
author = "Leabaneng Tawe and Emily MacDuffie and Mohan Narasimhamurthy and Qiao Wang and Simani Gaseitsiwe and Sikhulile Moyo and Ishmael Kasvosve and Shin, {Sanghyuk S.} and Zetola, {Nicola M.} and Paganotti, {Giacomo M.} and Surbhi Grover",
note = "Funding Information: We would like to acknowledge the contributions of Ms. Kebatshabile Ngoni, Mr. Lame Bakwenabatsile and Dr Erle Robertson, whose generous help, cooperation and technical assistance aided in the completion of our study. This work was supported by the Sub-Saharan African Collaborative HIV and Cancer Consortia-U54 [grant # 1U54 CA190158-01], the American Cancer Society International Fellowships for Beginning Investigators (ACSBI), Conquer Cancer Foundation Young Investigator Award, US National Institute of Allergy and Infectious Diseases (NIAID grant K01AI118559) and Penn Center for AIDS Research [grant # 5-P30-AI-045008-17]. This work was also supported by the Sub-Saharan African Network for TB/HIV Research Excellence (SANTHE), a DELTAS Africa Initiative [grant # DEL-15-006]. The DELTAS Africa Initiative is an independent funding scheme of the African Academy of Sciences (AAS)'s Alliance for Accelerating Excellence in Science in Africa (AESA) and supported by the New Partnership for Africa's Development Planning and Coordinating Agency (NEPAD Agency) with funding from the Wellcome Trust [grant # 107752/Z/15/Z]. The views expressed in this publication are those of the author(s) and are not necessarily those of NIH, AAS, NEPAD Agency, Wellcome Trust. Publisher Copyright: {\textcopyright} 2019 UICC",
year = "2020",
month = mar,
day = "15",
doi = "10.1002/ijc.32581",
language = "English (US)",
volume = "146",
pages = "1667--1673",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "6",
}