Humoral immunity to vimentin is associated with cardiac allograft injury in nonhuman primates

Agnes M. Azimzadeh, Steffen Pfeiffer, Guosheng S. Wu, Carsten Schröder, Hui Zhou, George L. Zorn, Marilyn Kehry, Geraldine G. Miller, Marlene L. Rose, Richard N. Pierson

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

Immunity to autologous protein has not previously been described following nonhuman primate cardiac transplant. Native hearts and cardiac allografts from cynomolgus monkeys were assessed by immunohistology for vimentin, a highly conserved intermediate filament protein. IgM and IgG to vimentin were measured in serial sera from untreated (n = 4) or cyclosporine (CsA)-treated (n = 8, 2 with ATG) cardiac allograft recipients, and in groups treated with anti-CD154 antibody with (n = 6) or without ATG (n = 28). IgM or IgG reactive with vimentin was elaborated within 30 days with unmodified acute rejection (3/4) or in CsA-treated animals (5/6). CD154 blockade did not prevent anti-vimentin IgM (14/28) but tended to delay the IgG response during therapy (anti-CD154: 8/28, p = 0.10 vs. CsA; anti-CD154+ATG: 2/6). CAV and alloantibody were seen in 25 of 26 animals with grafts surviving over 30 days, including seven animals without increasing anti-vimentin antibody. Anti-vimentin antibodies and vascular complement deposition were found in rejected hearts. Acute and chronic alloimmunity disrupt modulation of autoreactivity to vimentin through pathways, which are resistant to CsA, but may be partially regulated by CD154.

Original languageEnglish (US)
Pages (from-to)2349-2359
Number of pages11
JournalAmerican Journal of Transplantation
Volume5
Issue number10
DOIs
StatePublished - Oct 1 2005

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Keywords

  • Allotransplantation
  • Autoantigen
  • Costimulation
  • Heart-primate
  • Vimentin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Transplantation
  • Pharmacology (medical)

Cite this

Azimzadeh, A. M., Pfeiffer, S., Wu, G. S., Schröder, C., Zhou, H., Zorn, G. L., Kehry, M., Miller, G. G., Rose, M. L., & Pierson, R. N. (2005). Humoral immunity to vimentin is associated with cardiac allograft injury in nonhuman primates. American Journal of Transplantation, 5(10), 2349-2359. https://doi.org/10.1111/j.1600-6143.2005.01022.x