Hydrogen peroxide-mediated injury to cultured adult rat myocytes

Kuniharu Aida, Tomoya Onodera, Tatsuo Oguro, Muhammad Ashraf

Research output: Contribution to journalArticle

Abstract

In order to elucidate effects of oxidative stress by H2O2 on membrane permeability, morphology, lipid peroxidation and ATP content, the cultured adult rat myocytes were exposed to 100, 200,300 and 600 (J.M of H2O2 for 5-60 minutes. Both time and dose-dependent effects were observed. An increase in membrane permeability to trypan blue and lanthanum was observed in 93.5% of cells exposed to 300 μM H2O2 regardless of cell shape. After 60 minutes, 84.3% of cells had developed blebs regardless of cell shape. The pretreatment with 2.5 μM N,N′-diphenyl 1,4-phenylenediamine (DPPD), an antioxidant, or 20 mM deferoxamine, an iron chelator, prevented the increase in membrane permeability, bleb formation, and lipid peroxidation, but did not block ATP depletion. The metabolic inhibitors, iodoacetic acetate (10 mM) and carbonyl cyanide m-chlorophenyl hydrazone (10 μM) caused ATP depletion within five minutes but did not change the membrane permeability or increase bleb formation and lipid peroxidation. These data suggest that the damage by H2O2 is primarily attributed to lipid peroxidation which was not affected by ATP loss.

Original languageEnglish (US)
Pages (from-to)97-106
Number of pages10
JournalCardiovascular Pathobiology
Volume2
Issue number2
StatePublished - Dec 1 1997
Externally publishedYes

Keywords

  • ATP
  • Bleb formation
  • Lipid peroxidation
  • Morphology
  • Myocytes
  • O-derived radicals

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cardiology and Cardiovascular Medicine

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