Hydrogen sulfide as an endogenous regulator of vascular smooth muscle tone in trout

Ryan A. Dombkowski, Michael James Russell, Kenneth R. Olson

Research output: Contribution to journalArticle

164 Citations (Scopus)

Abstract

Hydrogen sulfide (H2S) is an endogenous vasodilator in mammals, but its presence and function in other vertebrates is unknown. We generated H2S from NaHS and examined the effects on isolated efferent branchial arteries from steelhead (stEBA) or rainbow (rtEBA) trout. H 2S concentration was measured colorimetrically (CM) and with ion-selective electrodes (ISE) in rainbow trout plasma. NaHS produced a triphasic response consisting of a relaxation (phase 1), constriction (phase 2), and relaxation (phase 3) in both unstimulated vessels and in stEBA precontracted with carbachol (Carb). Phase 1 and phase 3 in stEBA were decreased and phase 2 increased in unstimulated vessels by K+ ATP channel inhibition (glibenclamide), or a cocktail of inhibitors of cyclooxygenase, lipoxygenase, and cytochrome P-450 (indomethacin, esculetin, and clotrimazole). Inhibition of soluble guanylate cyclase with ODQ or NS-2028 inhibited phase 3 in stEBA, although NaHS decreased cGMP production by rtEBA. stEBA phase 2 contractions were partially inhibited by the myosin light chain kinase inhibitor, ML-9, but unaffected by L-type calcium channel inhibition (methoxyverapamil), whereas contraction in rtEBA was partially inhibited by nifedipine or removal of extracellular calcium. Phase 3 relaxations were more pronounced in stEBA precontracted with Carb and norepinephrine (NE) than those contracted by KC1 or K2SO4. stEBA phase 2 and phase 3 responses were dose dependent (EC50 = 1.1 ± 1.2 × 10-3 M and 6.7 ± 0.9 × 10-5 M, respectively; n = 7). NaHS was also vasoactive in steelhead bulbus arteriosus, celiacomesenteric arteries, and anterior cardinal veins. Rainbow trout plasma sulfide concentration was 4.0 ± 0.3 × 10-5 M, n = 4 (CM) and 3.8 ± 0.4 × 10-5 M, n = 9 (ISE); similar to phase 3 EC 50. Because NaHS has substantial vasoactive effects at physiological plasma concentrations, we propose that its soluble derivative, H2S, is a tonically active endogenous vasoregulator in trout.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume286
Issue number4 55-4
StatePublished - Apr 1 2004
Externally publishedYes

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Hydrogen Sulfide
Trout
Oncorhynchus mykiss
Vascular Smooth Muscle
Ion-Selective Electrodes
Carbachol
Arteries
Gallopamil
Clotrimazole
Myosin-Light-Chain Kinase
Lipoxygenase Inhibitors
L-Type Calcium Channels
Cyclooxygenase Inhibitors
Glyburide
Sulfides
Nifedipine
Vasodilator Agents
Constriction
Indomethacin
Cytochrome P-450 Enzyme System

Keywords

  • Cardiovascular
  • Fish
  • Guanosine 3′,5′-cyclic monophosphate
  • Vasoconstrictor
  • Vasodilator

ASJC Scopus subject areas

  • Physiology

Cite this

Hydrogen sulfide as an endogenous regulator of vascular smooth muscle tone in trout. / Dombkowski, Ryan A.; Russell, Michael James; Olson, Kenneth R.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 286, No. 4 55-4, 01.04.2004.

Research output: Contribution to journalArticle

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