Abstract
How vertebrate blood vessels sense acute hypoxia and respond either by constricting (hypoxic vasoconstriction) or dilating (hypoxic vasodilation) has not been resolved. In the present study we compared the mechanical and electrical responses of select blood vessels to hypoxia and H2S, measured vascular H2S production, and evaluated the effects of inhibitors of H2S synthesis and addition of the H2S precursor, cysteine, on hypoxic vasoconstriction and hypoxic vasodilation. We found that: (1) in all vertebrate vessels examined to date, hypoxia and H 2S produce temporally and quantitatively identical responses even though the responses vary from constriction (lamprey dorsal aorta; IDA), to dilation (rat aorta; rA), to multiphasic (rat and bovine pulmonary arteries; rPA and bPA, respectively). (2) The responses of IDA, rA and bPA to hypoxia and H2S appear competitive; in the presence of one stimulus, the response to the other stimulus is substantially or completely eliminated. (3) Hypoxia and H2S produce the same degree of cell depolarization in bPA. (4) H2S is constitutively synthesized by IDA and bPA vascular smooth muscle. (5) Inhibition of H2S synthesis inhibits the hypoxic response of IDA, rA, rPA and bPA. (6) Addition of the H2S precursor, cysteine, doubles hypoxic contraction in IDA, prolongs contraction in bPA and alters the re-oxygenation response of rA. These studies suggest that H 2S may serve as an O2 sensor/transducer in the vascular responses to hypoxia. In this model, the concentration of vasoactive H 2S in the vessel is governed by the balance between endogenous H 2S production and its oxidation by available O2.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4011-4023 |
| Number of pages | 13 |
| Journal | Journal of Experimental Biology |
| Volume | 209 |
| Issue number | 20 |
| DOIs | |
| State | Published - Oct 1 2006 |
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Keywords
- Cysteine metabolism
- Hydrogen sulfide metabolism
- Redox signaling
- Vascular smooth muscle
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Physiology
- Aquatic Science
- Animal Science and Zoology
- Molecular Biology
- Insect Science
Cite this
Hydrogen sulfide as an oxygen sensor/transducer in vertebrate hypoxic vasoconstriction and hypoxic vasodilation. / Olson, Kenneth R.; Dombkowski, Ryan A.; Russell, Michael J.; Doellman, Meredith M.; Head, Sally K.; Whitfield, Nathan L.; Madden, Jane A.
In: Journal of Experimental Biology, Vol. 209, No. 20, 01.10.2006, p. 4011-4023.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hydrogen sulfide as an oxygen sensor/transducer in vertebrate hypoxic vasoconstriction and hypoxic vasodilation
AU - Olson, Kenneth R.
AU - Dombkowski, Ryan A.
AU - Russell, Michael J.
AU - Doellman, Meredith M.
AU - Head, Sally K.
AU - Whitfield, Nathan L.
AU - Madden, Jane A.
PY - 2006/10/1
Y1 - 2006/10/1
N2 - How vertebrate blood vessels sense acute hypoxia and respond either by constricting (hypoxic vasoconstriction) or dilating (hypoxic vasodilation) has not been resolved. In the present study we compared the mechanical and electrical responses of select blood vessels to hypoxia and H2S, measured vascular H2S production, and evaluated the effects of inhibitors of H2S synthesis and addition of the H2S precursor, cysteine, on hypoxic vasoconstriction and hypoxic vasodilation. We found that: (1) in all vertebrate vessels examined to date, hypoxia and H 2S produce temporally and quantitatively identical responses even though the responses vary from constriction (lamprey dorsal aorta; IDA), to dilation (rat aorta; rA), to multiphasic (rat and bovine pulmonary arteries; rPA and bPA, respectively). (2) The responses of IDA, rA and bPA to hypoxia and H2S appear competitive; in the presence of one stimulus, the response to the other stimulus is substantially or completely eliminated. (3) Hypoxia and H2S produce the same degree of cell depolarization in bPA. (4) H2S is constitutively synthesized by IDA and bPA vascular smooth muscle. (5) Inhibition of H2S synthesis inhibits the hypoxic response of IDA, rA, rPA and bPA. (6) Addition of the H2S precursor, cysteine, doubles hypoxic contraction in IDA, prolongs contraction in bPA and alters the re-oxygenation response of rA. These studies suggest that H 2S may serve as an O2 sensor/transducer in the vascular responses to hypoxia. In this model, the concentration of vasoactive H 2S in the vessel is governed by the balance between endogenous H 2S production and its oxidation by available O2.
AB - How vertebrate blood vessels sense acute hypoxia and respond either by constricting (hypoxic vasoconstriction) or dilating (hypoxic vasodilation) has not been resolved. In the present study we compared the mechanical and electrical responses of select blood vessels to hypoxia and H2S, measured vascular H2S production, and evaluated the effects of inhibitors of H2S synthesis and addition of the H2S precursor, cysteine, on hypoxic vasoconstriction and hypoxic vasodilation. We found that: (1) in all vertebrate vessels examined to date, hypoxia and H 2S produce temporally and quantitatively identical responses even though the responses vary from constriction (lamprey dorsal aorta; IDA), to dilation (rat aorta; rA), to multiphasic (rat and bovine pulmonary arteries; rPA and bPA, respectively). (2) The responses of IDA, rA and bPA to hypoxia and H2S appear competitive; in the presence of one stimulus, the response to the other stimulus is substantially or completely eliminated. (3) Hypoxia and H2S produce the same degree of cell depolarization in bPA. (4) H2S is constitutively synthesized by IDA and bPA vascular smooth muscle. (5) Inhibition of H2S synthesis inhibits the hypoxic response of IDA, rA, rPA and bPA. (6) Addition of the H2S precursor, cysteine, doubles hypoxic contraction in IDA, prolongs contraction in bPA and alters the re-oxygenation response of rA. These studies suggest that H 2S may serve as an O2 sensor/transducer in the vascular responses to hypoxia. In this model, the concentration of vasoactive H 2S in the vessel is governed by the balance between endogenous H 2S production and its oxidation by available O2.
KW - Cysteine metabolism
KW - Hydrogen sulfide metabolism
KW - Redox signaling
KW - Vascular smooth muscle
UR - http://www.scopus.com/inward/record.url?scp=33749181930&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33749181930&partnerID=8YFLogxK
U2 - 10.1242/jeb.02480
DO - 10.1242/jeb.02480
M3 - Article
C2 - 17023595
AN - SCOPUS:33749181930
VL - 209
SP - 4011
EP - 4023
JO - Journal of Experimental Biology
JF - Journal of Experimental Biology
SN - 0022-0949
IS - 20
ER -