Hydrophilicity of dentin bonding systems influences in vitro Streptococcus mutans biofilm formation

Eugenio Brambilla, Andrei Ionescu, Annalisa Mazzoni, Milena Cadenaro, Massimo Gagliani, Monica Ferraroni, Franklin Tay, David Pashley, Lorenzo Breschi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objective To evaluate in vitro Streptococcus mutans (S. mutans) biofilm formation on the surface of five light-curing experimental dental bonding systems (DBS) with increasing hydrophilicity. The null hypothesis tested was that resin chemical composition and hydrophilicity does not affect S. mutans biofilm formation. Methods Five light-curing versions of experimental resin blends with increasing hydrophilicity were investigated (R1, R 2, R3, R4 and R5). R1 and R2 contained ethoxylated BisGMA/TEGDMA or BisGMA/TEGDMA, respectively, and were very hydrophobic, were representative of pit-and-fissure bonding agents. R3 was representative of a typical two-step etch-and-rinse adhesive, while R4 and R5 were very hydrophilic resins analogous to self-etching adhesives. Twenty-eight disks were prepared for each resin blend. After a 24 h-incubation at 37°C, a multilayer monospecific biofilm of S. mutans was obtained on the surface of each disk. The adherent biomass was determined using the MTT assay and evaluated morphologically with confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Results R2 and R3 surfaces showed the highest biofilm formation while R1 and R4 showed a similar intermediate biofilm formation. R5 was more hydrophilic and acidic and was significantly less colonized than all the other resins. A significant quadratic relationship between biofilm formation and hydrophilicity of the resin blends was found. CLSM and SEM evaluation confirmed MTT assay results.

Original languageEnglish (US)
Pages (from-to)926-935
Number of pages10
JournalDental Materials
Volume30
Issue number8
DOIs
StatePublished - Aug 2014

Fingerprint

Streptococcus mutans
Hydrophilicity
Biofilms
Dentin
Hydrophobic and Hydrophilic Interactions
Resins
Confocal Microscopy
Adhesives
Electron Scanning Microscopy
Dental Bonding
Curing
Assays
Microscopic examination
Scanning
Light
Scanning electron microscopy
Lasers
Biomass
In Vitro Techniques
Etching

Keywords

  • Biofilms
  • Dentin bonding systems
  • Hydrophilicity
  • Streptococcus mutans

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Brambilla, E., Ionescu, A., Mazzoni, A., Cadenaro, M., Gagliani, M., Ferraroni, M., ... Breschi, L. (2014). Hydrophilicity of dentin bonding systems influences in vitro Streptococcus mutans biofilm formation. Dental Materials, 30(8), 926-935. https://doi.org/10.1016/j.dental.2014.05.009

Hydrophilicity of dentin bonding systems influences in vitro Streptococcus mutans biofilm formation. / Brambilla, Eugenio; Ionescu, Andrei; Mazzoni, Annalisa; Cadenaro, Milena; Gagliani, Massimo; Ferraroni, Monica; Tay, Franklin; Pashley, David; Breschi, Lorenzo.

In: Dental Materials, Vol. 30, No. 8, 08.2014, p. 926-935.

Research output: Contribution to journalArticle

Brambilla, E, Ionescu, A, Mazzoni, A, Cadenaro, M, Gagliani, M, Ferraroni, M, Tay, F, Pashley, D & Breschi, L 2014, 'Hydrophilicity of dentin bonding systems influences in vitro Streptococcus mutans biofilm formation', Dental Materials, vol. 30, no. 8, pp. 926-935. https://doi.org/10.1016/j.dental.2014.05.009
Brambilla E, Ionescu A, Mazzoni A, Cadenaro M, Gagliani M, Ferraroni M et al. Hydrophilicity of dentin bonding systems influences in vitro Streptococcus mutans biofilm formation. Dental Materials. 2014 Aug;30(8):926-935. https://doi.org/10.1016/j.dental.2014.05.009
Brambilla, Eugenio ; Ionescu, Andrei ; Mazzoni, Annalisa ; Cadenaro, Milena ; Gagliani, Massimo ; Ferraroni, Monica ; Tay, Franklin ; Pashley, David ; Breschi, Lorenzo. / Hydrophilicity of dentin bonding systems influences in vitro Streptococcus mutans biofilm formation. In: Dental Materials. 2014 ; Vol. 30, No. 8. pp. 926-935.
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abstract = "Objective To evaluate in vitro Streptococcus mutans (S. mutans) biofilm formation on the surface of five light-curing experimental dental bonding systems (DBS) with increasing hydrophilicity. The null hypothesis tested was that resin chemical composition and hydrophilicity does not affect S. mutans biofilm formation. Methods Five light-curing versions of experimental resin blends with increasing hydrophilicity were investigated (R1, R 2, R3, R4 and R5). R1 and R2 contained ethoxylated BisGMA/TEGDMA or BisGMA/TEGDMA, respectively, and were very hydrophobic, were representative of pit-and-fissure bonding agents. R3 was representative of a typical two-step etch-and-rinse adhesive, while R4 and R5 were very hydrophilic resins analogous to self-etching adhesives. Twenty-eight disks were prepared for each resin blend. After a 24 h-incubation at 37°C, a multilayer monospecific biofilm of S. mutans was obtained on the surface of each disk. The adherent biomass was determined using the MTT assay and evaluated morphologically with confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Results R2 and R3 surfaces showed the highest biofilm formation while R1 and R4 showed a similar intermediate biofilm formation. R5 was more hydrophilic and acidic and was significantly less colonized than all the other resins. A significant quadratic relationship between biofilm formation and hydrophilicity of the resin blends was found. CLSM and SEM evaluation confirmed MTT assay results.",
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AU - Ionescu, Andrei

AU - Mazzoni, Annalisa

AU - Cadenaro, Milena

AU - Gagliani, Massimo

AU - Ferraroni, Monica

AU - Tay, Franklin

AU - Pashley, David

AU - Breschi, Lorenzo

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N2 - Objective To evaluate in vitro Streptococcus mutans (S. mutans) biofilm formation on the surface of five light-curing experimental dental bonding systems (DBS) with increasing hydrophilicity. The null hypothesis tested was that resin chemical composition and hydrophilicity does not affect S. mutans biofilm formation. Methods Five light-curing versions of experimental resin blends with increasing hydrophilicity were investigated (R1, R 2, R3, R4 and R5). R1 and R2 contained ethoxylated BisGMA/TEGDMA or BisGMA/TEGDMA, respectively, and were very hydrophobic, were representative of pit-and-fissure bonding agents. R3 was representative of a typical two-step etch-and-rinse adhesive, while R4 and R5 were very hydrophilic resins analogous to self-etching adhesives. Twenty-eight disks were prepared for each resin blend. After a 24 h-incubation at 37°C, a multilayer monospecific biofilm of S. mutans was obtained on the surface of each disk. The adherent biomass was determined using the MTT assay and evaluated morphologically with confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Results R2 and R3 surfaces showed the highest biofilm formation while R1 and R4 showed a similar intermediate biofilm formation. R5 was more hydrophilic and acidic and was significantly less colonized than all the other resins. A significant quadratic relationship between biofilm formation and hydrophilicity of the resin blends was found. CLSM and SEM evaluation confirmed MTT assay results.

AB - Objective To evaluate in vitro Streptococcus mutans (S. mutans) biofilm formation on the surface of five light-curing experimental dental bonding systems (DBS) with increasing hydrophilicity. The null hypothesis tested was that resin chemical composition and hydrophilicity does not affect S. mutans biofilm formation. Methods Five light-curing versions of experimental resin blends with increasing hydrophilicity were investigated (R1, R 2, R3, R4 and R5). R1 and R2 contained ethoxylated BisGMA/TEGDMA or BisGMA/TEGDMA, respectively, and were very hydrophobic, were representative of pit-and-fissure bonding agents. R3 was representative of a typical two-step etch-and-rinse adhesive, while R4 and R5 were very hydrophilic resins analogous to self-etching adhesives. Twenty-eight disks were prepared for each resin blend. After a 24 h-incubation at 37°C, a multilayer monospecific biofilm of S. mutans was obtained on the surface of each disk. The adherent biomass was determined using the MTT assay and evaluated morphologically with confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Results R2 and R3 surfaces showed the highest biofilm formation while R1 and R4 showed a similar intermediate biofilm formation. R5 was more hydrophilic and acidic and was significantly less colonized than all the other resins. A significant quadratic relationship between biofilm formation and hydrophilicity of the resin blends was found. CLSM and SEM evaluation confirmed MTT assay results.

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